Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting content of water-soluble ingredients in salvia miltiorrhiza medicinal material and application of method

A determination method, water-soluble technology, applied in the field of traditional Chinese medicine detection

Inactive Publication Date: 2015-02-25
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
View PDF9 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the simultaneous determination and detection of the contents and fingerprints of multiple components of Danshen

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting content of water-soluble ingredients in salvia miltiorrhiza medicinal material and application of method
  • Method for detecting content of water-soluble ingredients in salvia miltiorrhiza medicinal material and application of method
  • Method for detecting content of water-soluble ingredients in salvia miltiorrhiza medicinal material and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Embodiment 1: The method for determining the content of water-soluble components in Salvia miltiorrhiza medicinal materials is: according to ultra-high performance liquid chromatography, measure according to the following steps:

[0099] (1) Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; use 0.026% phosphoric acid aqueous solution as mobile phase A, acetonitrile as mobile phase B, A: B=85~10:15~ 90, Gradient elution: 0~5min: A: B=85~75:15~25, 5~8min: A:B=75~70:25~30, 8~12min: A:B=70~50: 30~50, 12~12.01min: A:B=50~10:50~90, 12.01~17min: A:B=10:90; detection wavelength is 276nm; flow rate is 0.1mL·min-1; column temperature is 25°C; the number of theoretical plates calculated based on the peak of salvianolic acid B should not be less than 2000;

[0100] (2) Preparation of reference substance solution: take appropriate amount of reference substances of Danshensu, protocatechualdehyde, rosmarinic acid, salvianolic ac...

Embodiment 2

[0103] Embodiment 2: The method for determining the content of water-soluble components in Danshen medicinal materials is: according to ultra-high performance liquid chromatography, measure according to the following steps:

[0104](1) Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; use 0.026% phosphoric acid aqueous solution as mobile phase A, acetonitrile as mobile phase B, A: B=85~10:15~ 90, Gradient elution: 0~5min: A: B=85~75:15~25, 5~8min: A:B=75~70:25~30, 8~12min: A:B=70~50: 30~50, 12~12.01min: A:B=50~10:50~90, 12.01~17min: A:B=10:90; detection wavelength is 306nm; flow rate is 0.5mL·min-1; column temperature is 35°C; the number of theoretical plates calculated based on the peak of salvianolic acid B should not be less than 2000;

[0105] (2) Preparation of reference substance solution: take appropriate amount of reference substances of Danshensu, protocatechualdehyde, rosmarinic acid, salvianolic acid B and salv...

Embodiment 3

[0108] Embodiment 3: The determination method of water-soluble component content in Danshen medical material is: according to ultra-high performance liquid chromatography, measure according to the following steps:

[0109] (1) Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; use 0.026% phosphoric acid aqueous solution as mobile phase A, acetonitrile as mobile phase B, A: B=85~10:15~ 90, Gradient elution: 0~5min: A: B=85~75:15~25, 5~8min: A:B=75~70:25~30, 8~12min: A:B=70~50: 30~50, 12~12.01min: A:B=50~10:50~90, 12.01~17min: A:B=10:90; detection wavelength is 286nm; flow rate is 0.2mL·min-1; column temperature is 30°C; the number of theoretical plates calculated based on the peak of salvianolic acid B should not be less than 2000;

[0110] (2) Preparation of reference substance solution: take appropriate amount of reference substances of Danshensu, protocatechualdehyde, rosmarinic acid, salvianolic acid B and salvianolic a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for detecting the content of water-soluble ingredients in a salvia miltiorrhiza medicinal material and an application of the method. The method is an ultra-performance liquid chromatography method by using reference substances of tanshinol, protocatechualdehyde, rosmarinic acid, salvianolic acid B and salvianolic acid A as reference and 0.026% phosphoric acid aqueous solution-acetonitrile in a ratio being (85-10):(15-90) as a moving phase for gradient elution. The method for detecting the content of water-soluble ingredients in a salvia miltiorrhiza medicinal material can be used for simultaneously detecting the content of multiple water-soluble ingredients in a salvia miltiorrhiza medicinal material, and can be applied to detection of salvia miltiorrhiza medicinal material fingerprint spectrum. The method has a good effect for separating water-soluble ingredients in the salvia miltiorrhiza medicinal material, and has the characteristics of quickness, accuracy, high reproducibility and high recovery rate, and has strong specificity, high precision, good repeatability, high stability and accurate measurement result. By applying the method, multiple peaks of the fingerprint spectrum can be detected, and reflected information is relatively complete; and the peaks have good shapes, can be easily identified, have high similarity, and are accurate and reliable.

Description

technical field [0001] The invention relates to a method for determining the content of water-soluble components in salvia miltiorrhiza medicinal materials and an application thereof, belonging to the technical field of traditional Chinese medicine detection. Background technique [0002] Salvia (Latin scientific name: Salvia miltiorrhiza Bunge) is also known as red ginseng, purple miltiorrhiza, red root, etc. The root is used as medicine, containing tanshinone, which is a strong menstruation agent. It has the effects of removing blood stasis, promoting regeneration, promoting blood circulation, and regulating menstruation. It is an important medicine for gynecology. pain. It has a good effect on the treatment of coronary heart disease. In addition, it also treats neurasthenia, insomnia, joint pain, anemia, mastitis, lymphadenitis, arthritis, sores, boils, pain and swelling, erysipelas, acute and chronic hepatitis, nephritis, bruises, hepatosplenomegaly in advanced schisto...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02
Inventor 张观福
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com