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Method for determining content of ochratoxin A in juice

A technology of ochratoxin and fruit juice, which is applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of poor feasibility, poor determination selectivity, difficulty in meeting analysis requirements, etc., and achieve the effect of simple method and high sensitivity

Inactive Publication Date: 2015-03-04
CHINA AGRI UNIV
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Problems solved by technology

However, the total emission fluorescence value is measured, and the selectivity for single substance determination in complex matrices is poor. Since the fluorescence spectrum of ochratoxin A in fruit juice mixes and overlaps with the spectrum of complex sample matrices, this method can be directly used to detect ochratoxin in it without separation. The feasibility of toxin A content is poor, that is, the conventional fluorescence analysis method is difficult to meet the analysis requirements

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  • Method for determining content of ochratoxin A in juice
  • Method for determining content of ochratoxin A in juice
  • Method for determining content of ochratoxin A in juice

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Embodiment

[0055] 1. Operation steps

[0056] Step (1), based on the parallel factor method (PARAFAC), establish a calibration model for quantitative analysis based on standard products

[0057] 1). Preparation of standard products:

[0058] Take 1mg of ochratoxin A standard substance and completely dissolve it with chromatography grade methanol, and dilute to 50mL (20μg / mL, store in the dark at -20°C) as the standard stock solution of ochratoxin A.

[0059] Take 0.5mL ochratoxin A standard stock solution, dilute to 100mL with methanol, with a concentration of 100ng / mL (stored in the dark at 4℃) for later use; prepare a series of ochratoxin A solutions with different concentrations with dilute sodium bicarbonate solution , Scan the 3D fluorescence intensity of the samples one by one, and investigate the linear range. The correlation coefficient is 0.99 within the concentration range of 0.27~3.24ng / mL, and the linear relationship is good for quantitative analysis.

[0060] 2) Preparation of calibr...

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Abstract

The invention discloses a method for determining the content of ochratoxin A in juice. The method comprises the following steps: pretreating a sample by adopting a simple liquid-liquid extraction and purification step; scanning with parameters including optimized scanning wavelengths, scanning intervals and the like, and acquiring three-dimensional fluorescent data of a standard product and the sample; carrying out mathematic separation treatment on the obtained data by adopting a parallel factor analyzing method (PARAFAC); establishing a correction model by using the standard product with the known concentration and by combining mathematic separation with chemical and physical separation; and predicating a component to be detected under the conditions that unknown and uncorrected background interferences are included and spectrums are seriously overlapped. The method is simple and rapid, and has the high sensitivity; and the content of ochratoxin in the juice can be determined under the unknown background interferences. The method belongs to the field of food safety.

Description

Technical field [0001] The invention relates to a method for the content of ochratoxin A, in particular to a method for determining the content of ochratoxin A in fruit juice based on a three-dimensional fluorescence second-order correction method, and belongs to the field of food safety. technical background [0002] Ochratoxin A (OTA) is a toxic metabolite produced by fungi. It is commonly found in grains and their products, coffee, fruits and their products. The International Agency for Research on Cancer (IARC) has identified it as a 2B carcinogen. There is currently no clear limit standard for ochratoxin A in fruit juices in my country, especially the rapid analysis and detection methods are rarely studied. [0003] The main detection methods for ochratoxin A include thin layer chromatography, enzyme-linked immunoassay, immunoaffinity column chromatography purification fluorescence spectrophotometry, solid phase extraction high performance liquid chromatography, liquid liquid ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 王军冯清清林亚青陈敏
Owner CHINA AGRI UNIV
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