Bacillus cereus NY5 capable of effectively inhibiting tilapia-source streptococcus agalactiae

The technology of Bacillus cereus and Bacillus cereus is applied in the field of Bacillus cereus strain NY5 with high activity, which can solve the problems such as the limitation of denitrification performance, and achieve the effect of broadening the application and strengthening the application value.

Active Publication Date: 2015-03-11
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Application Information

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Problems solved by technology

[0004] Sufficient supply of oxygen is one of the important conditions for intensive farming, and most of the traditional denit

Method used

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  • Bacillus cereus NY5 capable of effectively inhibiting tilapia-source streptococcus agalactiae
  • Bacillus cereus NY5 capable of effectively inhibiting tilapia-source streptococcus agalactiae
  • Bacillus cereus NY5 capable of effectively inhibiting tilapia-source streptococcus agalactiae

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Embodiment Construction

[0015] Attached below picture The present invention will be further described in the following examples and examples, but the following examples should not be construed as limiting the present invention.

[0016] 1. Screening of Bacillus cereus NY5

[0017] (1) Materials

[0018] 1. The samples used in the test were collected from the intestines of tilapia cultured in ponds of the Pearl River Fisheries Research Institute of the Chinese Academy of Fishery Sciences.

[0019] 2. Culture medium:

[0020] Beef extract peptone liquid medium: peptone 10g / L, beef extract 5g / L, NaCl 5g / L, pH 7.0.

[0021] Beef extract peptone solid medium: add agar 15-20g / L to beef extract peptone liquid medium.

[0022] Beef extract peptone high-salt medium: increase the amount of NaCl to 30g / L in the beef extract peptone solid medium.

[0023] BTB medium: asparagine 1g / L, KNO 3 1g / L, KH 2 PO 4 1g / L, FeCl 2 ·6H 2 O 0.05g / L, CaCl 2 2H 2 O 0.2g / L, MgSO 4 ·7H 2 O 1g / L, BTB 1g / L, agar 20g / L...

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Abstract

The invention relates to bacillus cereus NY5 capable of effectively inhibiting tilapia-source streptococcus agalactiae. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) and the preservation number is CGMCC No.9372. The bacillus cereus is aerobic bacteria and can effectively inhibit growth of tilapia-source streptococcus agalactiae. Through a nitrate nitrogen experiment, removal efficiency can reach 100%. The strain also has functions of casein hydrolysate and starch and has passed safety detection. In a water body with concentration of bacillus cereus NY5 being 2*107cfu/ml and under the condition of injecting 100 microliters of a NY5 bacterium solution at the concentration of 2.1*106cfu/ml, no death or other abnormal phenomena happen to tilapia. According to the invention, a useful strain source is provided for effectively inhibiting growth of tilapia-source streptococcus agalactiae and efficiently degrading nitrite nitrogen, protolysate and starch under the aerobic condition, and the application range of bacillus cereus in the aspect of functions is widened. The bacillus cereus NY5 has high application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a highly active bacillus cereus strain NY5 (Bacillus cereus). Background technique [0002] Tilapia belongs to the order Perciformes, the suborder Perciformes, the family Cichlidae, and the genus Tilapia. Because tilapia has the advantages of omnivorous, fast growth, strong fecundity and wide adaptability, it has become the main cultured fish in the world. my country is the world's largest producer and exporter of tilapia. In 2013, the output was 1.5 million tons, the processed volume was more than 300,000 tons, and the export value was more than 1 billion US dollars. However, since 2009, there have been frequent outbreaks of diseases in tilapia farming in my country, resulting in huge losses, which have seriously affected the sustainable development of the tilapia industry, the most serious of which is streptococcal disease. The disease has the characteristics of stron...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A61K35/742A61P31/04C12R1/085
CPCA61K35/742C02F3/34C12N1/205C12R2001/085
Inventor 卢迈新刘观斌王淼刘志刚可小丽高风英曹建萌朱华平
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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