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Method for extracting fucosan sulfate and fucoxanthin from kelp

A technology of fucoidan sulfate and fucoxanthin, which is applied in the direction of organic chemistry, can solve the problems of long extraction time of fucoidan sulfate and fucoxanthin, low product yield, environmental pollution, etc. Achieve the effect of saving organic solvent, high yield and short extraction time

Active Publication Date: 2016-08-24
WEIHAI RENSHENG PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the existing technical problems of extracting fucoidan sulfate and fucoxanthin from kelp with long extraction time, high cost, serious environmental pollution and low product yield, and provides a short extraction time, low cost Method for extracting fucoidan sulfate and fucoxanthin from kelp with low cost, good environmental protection effect and high product yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Wash and pulverize kelp, take 10kg and put it into a subcritical extraction tank, then add 30kg of extractant deionized water to the subcritical extraction tank, and extract for 30min at a temperature of 130°C and a pressure of 1MPa.

[0016] (2) Heat the extract at 100°C into a settling tank, cool the filtrate to 4°C in the settling tank and let it stand for 12 hours, then filter to obtain insoluble matter, and dry the insoluble matter to obtain fucoxanthin Crude.

[0017] (3) Concentrate the filtrate by ultrafiltration with a hollow cellulose membrane with a molecular weight cut-off of 3000, collect the concentrate, add 5% trichloroacetic acid by volume to the concentrate to remove protein, and centrifuge.

[0018] (4) adding the ethanol solution that the volume percentage is 95% in the centrifugal liquid, make the ethanol content in the solution be 20%, leave standstill for 8 hours, remove precipitation; The ethanol content in the solution was 60%, and it was le...

Embodiment 2

[0020] (1) Wash and crush the kelp, put 20kg into a subcritical extraction tank, then add 100kg of extractant deionized water to the subcritical extraction tank, and extract for 20min at a temperature of 140°C and a pressure of 1.5MPa.

[0021] (2) Heat the extract at 100°C into a settling tank, cool the filtrate to 4°C in the settling tank and let it stand for 8 hours, then filter to obtain insoluble matter, and dry the insoluble matter to obtain fucoxanthin Crude.

[0022] (3) Concentrate the filtrate by ultrafiltration with a hollow cellulose membrane with a molecular weight cut-off of 5000, collect the concentrated solution, add 6% trichloroacetic acid to the concentrated solution to remove protein, centrifuge, and add 95% trichloroacetic acid to the centrifuged liquid % ethanol solution, so that the ethanol content in the solution is 30%, let it stand for 10 hours, and remove the precipitate; then add 95% ethanol solution to the supernatant to make the ethanol content in ...

Embodiment 3

[0024] (1) Wash and pulverize the kelp, take 100kg and put it into a subcritical extraction tank, then add 600kg of extractant deionized water to the subcritical extraction tank, and extract for 25min at a temperature of 125°C and a pressure of 1.2MPa.

[0025] (2) Heat the extract at 95°C into a settling tank, cool the filtrate to 4°C in the settling tank and let it stand for 11 hours, then filter to obtain insoluble matter, and dry the insoluble matter to obtain fucoxanthin Crude.

[0026] (3) Concentrate the filtrate by ultrafiltration with a hollow cellulose membrane with a molecular weight cut-off of 5000, collect the concentrate, add 7% trichloroacetic acid by volume to the concentrate to remove protein, and centrifuge.

[0027] (4) adding the ethanol solution that the volume percentage is 95% in the centrifugal liquid, making the ethanol content in the solution be 25%, leave standstill 9 hours, remove precipitation; The ethanol content in the solution was 70%, and it w...

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Abstract

The invention relates to a method for extracting fucoidan and fucoxanthin from kelp. The method solves the technical problems that an existing method for extracting the fucoidan and the fucoxanthine from the kelp is long in extraction time, high in cost, serious in environment pollution and low in product yield. The method comprises the steps that the kelp is cleaned, smashed and put into an extraction tank together with a certain proportion of water, extraction is conducted at high temperature and under high pressure, hot filtration, cooling and sedimentation are conducted on an extraction solution, sediment is the fucoxanthin, and the fucoxanthin product is obtained after the drying process; then ultrafiltration concentration is conducted on supernatant liquid, filtrate is collected, trichloroacetic acid is added into the filtrate to remove protein, centrifugation is conducted, an ethanol solution is added into the supernatant liquid for fractional precipitation, sediment is collected, and the fucoidan is obtained after the vacuum drying process. The method can be used for extracting the fucoidan and the fucoxanthin from the kelp.

Description

technical field [0001] The invention relates to a method for extracting active substances in kelp, in particular to a method for extracting fucoidan sulfate and fucoxanthin from kelp. Background technique [0002] Fucoidan sulfate is a unique natural active polysaccharide containing sulfate in brown algae, which has anti-tumor, anti-coagulation, anti-virus, anti-thrombosis, lowering blood lipids, improving immunity, detoxifying heavy metal poisoning and anti-HIV. , has good medical application value, and is also an important raw material for food, cosmetics and health products. Fucoidan sulfate is rich in sources and low in cost. It has entered the market in Japan and the United States as a drug for the prevention and treatment of cancer and thrombosis. [0003] Fucoxanthin (fucoxanthin) has various active functions such as anti-tumor, anti-oxidation, anti-inflammation and weight loss. It is an important carotenoid component rich in kelp, which accounts for more than 60% of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C07D303/32
Inventor 苗其壮雷建都付东霖
Owner WEIHAI RENSHENG PHARMA GRP
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