Preparation method of chromosomes of adult epinephelus akaara

A red-spotted grouper and chromosome technology, applied in the field of cell biology, can solve the problems of long time, difficult acquisition and high cost, and achieve the effect of saving time and increasing the injection concentration
CN104483178AInactive Publication Date: 2015-04-01YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI +2
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Publication Date
2015-04-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of chromosomes of adult epinephelus akaara and belongs to the field of cell biology. The preparation method comprises the following steps: pretreatment of materials, preparation of kidney cell suspension, low-permeation treatment, fixation, dropping and dyeing, wherein the pretreatment of the materials comprises the steps of with an in vivo injection method of phytohemagglutinin (PHA), injecting PHA into the pectoral-fin base part of the experimental adult epinephelus akaara with 15 micrograms in each gram of the adult epinephelus akaara; after 24 hours, injecting colchicine solution with 2 micrograms in each gram of the adult epinephelus akaara; and after 2.5-3 hours, taking out the head kidney. The preparation method disclosed by the invention has the advantages that the method for one-time injection of PHA is adopted, simultaneously, the injection concentration is increased, and good effect can be obtained after 24 hours, so that the time is saved; and due to the characteristics of tissue cells of the epinephelus akaara, complete cells are not easily obtained when the low-permeation treatment is carried out in the prior art, so that the prior art is creatively changed and a clear chromosome image is obtained.
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Description

technical field

[0001] The invention belongs to the field of cell biology, and in particular relates to a method for preparing chromosomes of red-spotted grouper adults. Background technique

[0002] Chromosomal specimen preparation has the characteristics of simple operation, low price, and low requirement for experimental site. Steps such as slices have been widely used in many fields of biogenetics. However, due to the tissue characteristics of the red-spotted grouper itself, according to the conventional chromosome preparation method, it is difficult to obtain, the cost is high, the effect of making chromosome samples is poor, and the time is long. Contents of the invention

[0003] The technical problem to be solved by the present invention is to provide a method for preparing chromosomes of red-spotted grouper adult fish. The present invention improves the conventional chromosome preparation method, changes the hypotonic treatment time and pretreatment method, and o...

Claims

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