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Preparation Method of Chromosome of Red-spotted Grouper Adult

A red-spotted grouper and chromosome technology, applied in the field of cell biology, can solve the problems of long time, difficult acquisition, poor effect of chromosome samples, etc., and achieve the effect of saving time and increasing the injection concentration.

Inactive Publication Date: 2018-03-30
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI +2
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Problems solved by technology

However, due to the tissue characteristics of the red-spotted grouper, according to the conventional chromosome preparation method, it is difficult to obtain, the cost is high, the effect of making chromosome samples is poor, and the time is long

Method used

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  • Preparation Method of Chromosome of Red-spotted Grouper Adult
  • Preparation Method of Chromosome of Red-spotted Grouper Adult
  • Preparation Method of Chromosome of Red-spotted Grouper Adult

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Embodiment Construction

[0027] A method for preparing chromosomes of red-spotted grouper adult fish, which includes pre-treatment of materials, preparation of kidney cell suspension, hypotonic treatment, fixation, dropping and staining, and the specific steps are as follows:

[0028] (1) Pre-processing of materials

[0029] The pre-treatment of the material adopts the in vivo injection method of phytohemagglutinin, and injects PHA to the base of the pectoral fin of the experimental fish at a dose of 15 μg / g fish body weight, and injects colchicine solution at a dose of 2 μg / g fish body weight after 24 hours, about After 3 hours, the tail was docked to lose blood for 20 minutes, and the head kidney was removed;

[0030] (2) Preparation of kidney cell suspension

[0031] Wash the head kidney tissue in 0.8% (weight percent) normal saline to remove blood clots and other tissues, then place it in a petri dish filled with 0.8% (weight percent) normal saline, fully cut it into pieces, and then use four lay...

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Abstract

A method for preparing chromosomes of red-spotted grouper adult fish belongs to the field of cell biology, and it includes pre-treatment of materials, preparation of kidney cell suspension, hypotonic treatment, fixation, dripping and staining; the pre-treatment of materials is Using the phytohemagglutinin injection method in vivo, inject PHA at the base of the pectoral fin of the experimental fish at a dose of 15 μg / g fish body weight, and inject colchicine solution at a dose of 2 μg / g fish body weight after 24 hours, and after 2.5-3 hours , take out the head kidney. The present invention adopts the local method of one-time injection of phytohemagglutinin, increases the injection concentration at the same time, can obtain good effect after 24 hours, and saves time. In the present invention, due to the characteristics of tissue cells of the red-spotted grouper, it is difficult to obtain complete cells when the hypotonic treatment is performed according to the prior art. Therefore, the present invention creatively changes the prior art and obtains a clear chromosome picture.

Description

technical field [0001] The invention belongs to the field of cell biology, and in particular relates to a method for preparing chromosomes of red-spotted grouper adults. Background technique [0002] Chromosomal specimen preparation has the characteristics of simple operation, low price, and low requirement for experimental site. Steps such as slices have been widely used in many fields of biogenetics. However, due to the tissue characteristics of the red-spotted grouper itself, according to the conventional chromosome preparation method, it is difficult to obtain, the cost is high, the effect of making chromosome samples is poor, and the time is long. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for preparing chromosomes of red-spotted grouper adult fish. The present invention improves the conventional chromosome preparation method, changes the hypotonic treatment time and pretreatment method, and o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
Inventor 陈超刘莉孔祥迪李炎璐李娟徐万土陈建国于欢欢
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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