Method for creating jade rabbit pleated skirt Taishi goldfish
A technology of Thai lion goldfish and pleated skirt, applied in the field of fish molecular design and breeding, can solve the problems of weakening, loss and unrealization of the target fish traits
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0035] Example 1: Efficient breeding of pleated skirt Thai lion goldfish
[0036] S1. Seed cultivation: Raise freshly opened fry in a turnover box with a water temperature of 23-25°C and feed live Artemia 3 times a day. The turnover box is 60-100cm long, 40-60cm wide, 20-30cm high, and the water level is 15 -20cm is appropriate. Install a sponge filter on one side of the turnover box to filter, and place an oxygen increasing head next to it for oxygenation, and the air volume is appropriate. Clean the sponge filter once a week.
[0037] After the fry exceed 2cm in length, look down to select four-tailed fry, and then look sideways to remove the deformed and folded fry, and put the selected high-quality fry into a new container for rearing, with a density of about 2 fish per liter of water; the container is equipped with The 7-watt small water pump adds water by trickle, and the daily water intake is about 1 / 5 of the total water body. The three-layer turnover box is filtered ...
Embodiment 2
[0047] Example 2: Exploration of Gynogenetic Conditions
[0048] Two methods of gynogenesis were established, the proportion of normal fish produced by the two methods was compared, and the one with a higher rate of homozygosity was selected as the best gynogenesis condition for Thai lion goldfish.
[0049] 1. Meiotic gynogenesis
[0050] Take carp sperm to inactivate, mix inactivated sperm with pleated skirt Thai lion goldfish eggs, and then perform cold shock treatment. The cold shock treatment method is to develop fertilized eggs at a constant temperature of 24°C for 3-7 minutes, and then place them at 0°C Cold shock was carried out in ice water for 2 minutes to inhibit the discharge of the second polar body, so that the two gametes merged into one to form a tetraploid. After the cold shock treatment, the fertilized eggs hatched into meiotic Thai lion goldfish with jade rabbit traits.
[0051] 2. Mitotic Gynogenesis
Embodiment 3
[0057] Example 3: Creation of the Jade Rabbit Pleated Skirt Thai Lion Goldfish
[0058] S1. Target design: Sequence the gene of the pleated skirt Thai lion goldfish to obtain the DNA sequence and amino acid sequence of the gene that regulates the traits of the jade rabbit, and use the wildcard GG[G / A]? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? GG or CC? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? ? [T / C] CC designs a suitable knockout target on the exon of the DNA sequence of the gene that regulates the traits of the jade rabbit, builds a local genome database after obtaining the target, and uses blast software to search and verify the uniqueness of the target , after determining the uniqueness, the target sequence will be transcribed into gRNA in vitro; "?" in the wildcard character represents an arbitrary base, "[]" represents a match with any base in [], and " / " represents "or".
[0059] The gene regulating the traits of the jade rabbit is the oca2 gene, and th...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com