Unlock instant, AI-driven research and patent intelligence for your innovation.

Fingerprint based staphylococcus aureus identification method

A staphylococcus and fingerprint technology, applied in the field of staphylococcus aureus identification, can solve the problems of complicated operation, wrong interpretation, inability to popularize, etc., and achieves the effects of good specificity, short time-consuming and simple operation

Inactive Publication Date: 2015-04-01
HEBEI AGRICULTURAL UNIV.
View PDF4 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the domestic detection of Staphylococcus aureus in food mainly adopts traditional methods of isolation, cultivation, and biochemical identification, which are cumbersome to operate and have a long detection cycle, which can no longer meet the needs of rapid detection of pathogenic bacteria in food
However, molecular methods such as PCR and LAPM technology have relatively high technical content, have high requirements for personnel operation, instrument conditions, and sample testing environment, and are prone to false positive test results, which still cannot meet the needs of actual testing.
Although there are commercially available Staphylococcus aureus rapid test strips on the market, they cannot be widely used due to occasional errors in interpretation.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fingerprint based staphylococcus aureus identification method
  • Fingerprint based staphylococcus aureus identification method
  • Fingerprint based staphylococcus aureus identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] Comparison of the spectrum of common bacteria in food and the spectrum of Staphylococcus aureus and the identification of mixed bacteria

[0110] The foods that Staphylococcus aureus is often easily infected are mainly high-protein foods such as meat, eggs, and milk. These foods are also susceptible to infection by pathogenic microorganisms such as Escherichia coli, Salmonella, Shigella, Listeria monocytogenes, and Enterobacter sakazakii. Under the above determined Staphylococcus aureus culture method, pretreatment method and liquid chromatographic conditions, extract and screen out the bacterial cell extracts of the above-mentioned strains and Staphylococcus aureus respectively mixed with the above various cultures, Filter through a 0.45 μm microporous membrane. Precisely draw 20 μl of each test solution, inject the samples separately, record the chromatogram and retention time, compare with the spectrum of Staphylococcus aureus, and further obtain the feasibility of ...

Embodiment 2

[0112] Double-blind test to verify the accuracy of fingerprints

[0113] In order to exclude the influence of unintentional physical cues or observer bias on the research results. In this experiment, when verifying the accuracy of the fingerprint of Staphylococcus aureus in identifying the presence or absence of Staphylococcus aureus, a double-blind test was chosen. Without the experimenter's knowledge, the Staphylococcus aureus was randomly artificially polluted the milk samples, and the records were made. After the experiment was over and all the data were recorded and analyzed, the experimenter could know which milk samples were contaminated and which milk samples were contaminated. Samples are uncontaminated.

[0114] Buy pasteurized fresh milk from the market. According to the national standard GBT 4789.10-2010 "National Food Safety Standard Food Sanitation Microbiological Examination of Staphylococcus aureus", it is confirmed that it does not contain Staphylococcus aur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a fingerprint based staphylococcus aureus identification method. A somatic cell extract chromatogram is established for 34 staphylococcus aurei and 20 non-staphylococcus aurei with a high performance liquid chromatography method, and the characteristic peak of the staphylococcus aurei is determined through comparison; staphylococcus aureus protein A (SPA) is taken as an internal standard substance (characteristic peak), the relative retention time of the characteristic peak and a standard peak is taken as data foundation to establish staphylococcus aureus fingerprint, correlation coefficient distribution of two types of fingerprint are analyzed and compared, a limiting value is determined, and a fingerprint calculation method is established and used for strain identification. The method has certain feasibility on staphylococcus aureus identification and is simple to operate, short in time consumption and good in specificity, and a new stable technical platform is established for rapid identification and detection of staphylococcus aurei, and meanwhile, a brand-new identification method is developed for identification and detection of microorganisms.

Description

technical field [0001] The invention relates to the field of strain identification, in particular to a fingerprint-based identification method for staphylococcus aureus. Background technique [0002] Staphylococcus aureus (Staphyloccocus aureus SA) is recognized as an important foodborne pathogen in the world. It exists widely in nature, and as a common pathogenic bacteria of humans and animals, it mainly exists in the exposed nasal cavity, throat, and hair of humans and animals. On the one hand, Staphylococcus aureus can cause local suppurative inflammation, on the other hand, it can grow and multiply in contaminated food, produce enterotoxin, and cause food poisoning. Strengthening the detection of Staphylococcus aureus in food is the most effective way to solve the food safety problems caused by the bacteria contamination of food. At present, the domestic detection of Staphylococcus aureus in food mainly adopts traditional methods of isolation, cultivation, and biochemi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/88
Inventor 田益玲张伟马晓燕赵元煜于志彬
Owner HEBEI AGRICULTURAL UNIV.