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Detection method of babaowudan cinnabar stick

A detection method, the technology of eight treasures and five guts, applied in the field of medicine, can solve the problems of few detection methods, achieve clear spots, reduce interference, and reduce dosage

Inactive Publication Date: 2015-04-01
ANHUI MOYAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are few detection methods for various components in Babao Wudan medicinal ink, especially the detection methods for borneol and toad venom

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A detection method of eight treasures and five guts medicinal ink, comprising a detection method of borneol and a detection method of toad venom, the relevant steps are as follows:

[0016] (1) Borneol detection method: take an appropriate amount of Babao Wudan medicated ink, grind it finely, take 7g of powder, add 30ml of ether and shake well, ultrasonicate for 5min, the frequency is controlled at 70kHZ, filter for later use, and make the test solution, then Draw 2 ul of the filtrate and spot it on the silica gel G thin-layer plate, add the developing agent cyclohexane-ethyl acetate, the ratio is 17:3, develop, take it out, dry it in the air, and finally spray it with a 5% vanillin sulfuric acid solution of the color developing agent, The hot air is blown until the spots are clearly colored, and the testing of the test product is completed. In the detection method of borneol, a negative control sample without borneol should be set at the same time, and the detection met...

Embodiment 2

[0019] A detection method of eight treasures and five guts medicinal ink, comprising a detection method of borneol and a detection method of toad venom, the relevant steps are as follows:

[0020] (1) Borneol detection method: take an appropriate amount of Babao Wudan medicated ink, grind it finely, take 7.5g of powder, add 32ml of ether and shake well, ultrasonicate for 5.5min, the frequency is controlled at 85kHZ, filter for later use, and make the test solution , then draw 2ul of the filtrate and point it on the silica gel G thin-layer plate, add the developing agent cyclohexane-ethyl acetate, the ratio is 17:3, develop, take out, dry in the air, and finally spray with the color developing agent 6% vanillin sulfuric acid Solution, hot air is blown until the spots are clearly colored, and the test product detection is completed. In the borneol detection method, a negative control sample without borneol should be set at the same time, and its detection method is the same as th...

Embodiment 3

[0023] A detection method of eight treasures and five guts medicinal ink, comprising a detection method of borneol and a detection method of toad venom, the relevant steps are as follows:

[0024] (1) Borneol detection method: take an appropriate amount of Babao Wudan medicated ink, grind it finely, take 8g of powder, add 35ml of ether and shake well, ultrasonicate for 6min, and its frequency is controlled at 100kHZ, filter for later use, make the test solution, and then Draw 2 ul of the filtrate and spot it on the silica gel G thin-layer plate, add the developing agent cyclohexane-ethyl acetate, the ratio is 17:3, develop, take out, dry in the air, and finally spray with the 7% vanillin sulfuric acid solution of the color developing agent, The hot air is blown until the spots are clearly colored, and the testing of the test product is completed. In the detection method of borneol, a negative control sample without borneol should be set at the same time, and the detection metho...

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PUM

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Abstract

The invention provides a detection method of babaowudan cinnabar stick. The detection method comprises a borneol detection method and a bufotoxin detection method. Diethyl ether is adopted for extraction and taken as a test solution for detection, then dregs of decoction are taken as samples for a bufotoxin identification experiment, part of impurities in bufotoxin identification are removed, and further, the use amount of the samples is reduced. When amethanol extracting solution is taken as a test solution, more serious thin-layer chromatography interference is produced after development, and bufotoxin detection is affected; the methanol extracting solution is concentrated and passes a neutral aluminum oxide column, methanol is used for elution, a large quantity of impurities can be removed, and thin-layer chromatography interference can be reduced. A developer for bufotoxin detection adopts a toluene-acetone developer, the Rf (retardation factor) value is moderate, and spots are clear.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a detection method of eight treasures and five gall bladders medicinal ink. Background technique [0002] Babao Wudan Herbal Ink is a variety recorded in Volume 14 of the Ministry of Health’s Pharmaceutical Standards for Traditional Chinese Medicine Prescriptions. It is composed of twenty-two traditional Chinese medicines such as buffalo horn concentrated powder, antelope horn, and artificial musk. It has the effects of anti-inflammatory and detoxification, promoting blood circulation and relieving pain, cooling blood to stop bleeding, reducing swelling and softening hardness, antiseptic and astringent. It is clinically used for hematemesis, hemoptysis, epistaxis, blood in the stool, red and white dysentery, carbuncle sores, unknown swollen toxins, naughty ringworm, dermatitis, eczema, etc. Babao Wudan medicine ink is made of pure traditional Chinese medicine without any side e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
Inventor 张礼春胡一中
Owner ANHUI MOYAO PHARMA
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