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Application of exosome secreted by human umbilical cord mesenchymal stem cells to treating skin injury

A technology for skin damage and stem cells, which can be used in medical preparations containing active ingredients, skin diseases, and medical raw materials derived from mammals. effect of value

Active Publication Date: 2015-04-08
江苏澳洋生物医药研究院有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term application safety and storage and transportation difficulties of MSC after implantation in the body greatly limit its clinical application. It is very urgent to find biomaterials or stem cell products that can replace MSC in the repair of tissue damage.

Method used

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  • Application of exosome secreted by human umbilical cord mesenchymal stem cells to treating skin injury
  • Application of exosome secreted by human umbilical cord mesenchymal stem cells to treating skin injury
  • Application of exosome secreted by human umbilical cord mesenchymal stem cells to treating skin injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Separation and purification of exosome secreted by umbilical cord mesenchymal stem cells;

example 1

[0033] The main materials and sources used in example 1 are as follows respectively:

[0034] MSC culture reagents Low-sugar DMEM, fetal bovine serum (Gibco company product), trypsin (Sigma company product), carbon dioxide incubator (Forma company), serum-free medium (Shanghai Yikesai company)

[0035] Inverted microscope, biological microscope, ultra-clean bench, desktop centrifuge;

[0036] Heavy water (D2O, Shanghai Chuangsai Company), analytically pure sucrose (Guangzhou Chemical Reagent Factory), rabbit anti-human CD9 antibody (U.S. Bioworld Technology Company), rabbit anti-human CD81 antibody (U.S. Epitomics Company), BCA protein quantification kit, spicy Root peroxidase (HRP)-labeled goat anti-rabbit IgG secondary antibody (Beijing Kangwei Century Company), premixed HRP chemiluminescence substrate, 100-kDa MWCO ultrafiltration centrifuge tube, 0.22 μm sterile filter membrane (Millipore, USA) company); transmission electron microscope (FEI Tecnai 12, Philips Cor...

Embodiment 2

[0044] Example 2: Therapeutic effect of exosome secreted by umbilical cord mesenchymal stem cells on skin deep second-degree burns

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Abstract

The invention relates to an application of exosome secreted by human umbilical cord mesenchymal stem cells to treating skin injury and in particular relates to a medicinal action of vesica structure exosome which is secreted by human umbilical cord mesenchymal stem cells and has the diameter of about 100nm in the promotion of skin injury repair, belonging to the technical field of skin injury treatment. The exosome disclosed by the invention comes from the human umbilical cord mesenchymal stem cells. The test proves that the disclosed exosome serving as an important component secreted by the human umbilical cord mesenchymal stem cells has an obvious medicinal action in promoting repair in the aspects of skin injuries including burns and scalds and injury removal, and has the advantage of convenience in storage and transportation. Therefore, the exosome from the umbilical cord mesenchymal stem cells provides possibility for non-cell skin injury by taking the mesenchymal stem cells as the basis.

Description

Technical field [0001] The invention involves the application of EXOSOME secreted by human umbilical cords to treat skin damage. It specifically involves a puff structure with a diameter of about 100nm in the diameter of an umbilical cord album.The technical field of treatment. Background technique [0002] Skin injury is a common trauma for daily life.Skin injury is also one of the common wounds, accounting for 10%-30%of the war, and nuclear war can reach 90%.Burning and burns have high incidence and mortality.At present, large -scale skin injury treatment is mainly through systemic support treatment, wound cleansing, scabs, and surgical skin grafts. Although the traditional treatment method has certain effects, it is difficult to grasp, tension and infection of skin sources, tension and infection.waiting for solution. [0003] In recent years, the research on skin regeneration medicine based on stem cells, especially mesenchymal sTem Cell (MSC) has received widespread attention...

Claims

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Application Information

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IPC IPC(8): A61K35/51A61P17/00A61P17/02
CPCA61K35/28
Inventor 张斌许文荣钱晖朱伟严永敏王梅朱艳华史惠吴莉珺潘昭吉
Owner 江苏澳洋生物医药研究院有限责任公司
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