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RFLP method and kit for detecting SNP locus of MEF2C gene of cattle

A kit and scalper technology, applied in the field of molecular genetics, can solve the problems of few research reports and exploration, and achieve the effects of excellent genetic resources, low cost, and high detection accuracy

Inactive Publication Date: 2015-04-08
NORTHWEST A & F UNIV
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Problems solved by technology

[0005] At present, the research on MEF2C gene mainly focuses on the pathogenesis of mouse and human cardiac muscle and skeletal muscle. There are few related research reports in livestock and poultry, especially in cattle, and there is no relatively in-depth and systematic exploration.

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  • RFLP method and kit for detecting SNP locus of MEF2C gene of cattle
  • RFLP method and kit for detecting SNP locus of MEF2C gene of cattle
  • RFLP method and kit for detecting SNP locus of MEF2C gene of cattle

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Embodiment Construction

[0030] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, so that the public can have an overall and sufficient understanding of the content of the invention, rather than limiting the protection scope of the present invention.

[0031] The present invention designs primers according to the MEF2C gene sequence, respectively uses the blood genome DNA of two cattle breeds as templates, performs PCR amplification, compares it with the sequence published by the authoritative database after sequencing, and finds that the 50th position of intron 4 (IVS4+50T >C) There is a single nucleotide polymorphism. For the above SNP site, the present invention detects by artificially introducing a base mismatch at the 3' end of the upstream primer through primer design. When there is no mutation at this site, the restriction endonuclease Hind Ш recognition site will be formed at the mismatched position after PCR amplification of t...

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Abstract

The invention discloses a RFLP method and a kit for detecting an SNP locus of an MEF2C gene of cattle. The method comprises the following steps: by taking cattle genome DNA or DNA containing MEF2C gene sequences as a template, performing PCR amplification, digesting PCR amplification products by using restriction endonuclease Hind III, performing agarose gel electrophoresis on digested segments, and identifying the single nucleotide polymorphism of the NEF2C gene of cattle. As the MEF2C gene plays a significant role in the muscle development process and is tightly related to cattle growth and meat quality property, and SNP is related to multiple significant economic properties of cattle, the detection method disclosed by the invention can be applied to auxiliary selection of cattle meat growth marks, is simple and rapid to operate, low in cost and high in detection precision, and has good advantages in establishing cattle population with excellent genetic resources.

Description

technical field [0001] The invention belongs to the field of molecular genetics, in particular to the rapid detection of MEF2C (myocyte enhancer factor 2C) gene single nucleotide polymorphism (SNP) based on RFLP (restriction fragment length polymorphism). Background technique [0002] The so-called single nucleotide polymorphism (SNP) refers to the polymorphism caused by the replacement of a single nucleotide (A / T / C / G) in the genomic DNA sequence, including base insertion, deletion and repeat sequence Copy number changes. A SNP refers to a nucleotide change at a certain position in the genome, mainly consisting of a single base conversion (one pyrimidine for another pyrimidine or one purine for another purine) and transversions ( purine and pyrimidine exchange), and the SNPs caused by the former account for about 2 / 3. Various SNPs may be found in or near any known or unknown gene, which can be divided into gene coding region SNPs (cSNPs), gene peripheral SNPs (pSNPs) and i...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2535/138
Inventor 陈宏展召阳张国兴周雅婷黄永震雷初朝蓝贤勇胡沈荣
Owner NORTHWEST A & F UNIV
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