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A detection method for metalaxyl residues in eggplant

A detection method, the technology of metalaxyl, is applied in the field of detection of metalaxyl residues in eggplant, which can solve the problems of complex operation in the extraction process, and achieve the effect of high extraction rate and simple and easy-to-operate detection method

Active Publication Date: 2016-09-28
SUZHOU APP SCI ACAD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The extraction process of the commonly used method is complex and requires a simple detection method for the detection of metalaxyl residues in eggplant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In the present embodiment, a kind of detection method of metalaxyl residue in eggplant comprises the following steps:

[0023] (1) Sample processing: Put the picked eggplants in a crusher and crush them to obtain eggplant pulp;

[0024] (2) Extraction: Weigh 20g of eggplant pulp into an Erlenmeyer flask, add 100mL of ethyl acetate and acetone mixture, shake and extract at a temperature of 40°C for 60min, filter with suction to obtain a clear liquid, concentrate to nearly dry, and nitrogen Blow to dryness, redissolve with 2mL acetonitrile for later use;

[0025] (3) Column separation: wash the HLB column with 5mL ethyl acetate, then wash the column with 5ml acetone, load the sample, rinse the column with 10mL of acetonitrile and water mixture with a volume ratio of 1:2, and finally wash the column with 10ml eluted with acetonitrile, collected the eluent, rotary evaporated to 1ml, blown dry with nitrogen, and dilute to 2ml with acetonitrile for detection;

[0026] (4) D...

Embodiment 2

[0029] In the present embodiment, a kind of detection method of metalaxyl residue in eggplant comprises the following steps:

[0030] (1) Sample processing: Put the picked eggplants in a crusher and crush them to obtain eggplant pulp;

[0031] (2) Extraction: Weigh 20g of eggplant pulp into an Erlenmeyer flask, add 100mL of ethyl acetate and acetone mixture, shake and extract for 60min at a temperature of 35°C, filter to obtain a clear liquid, concentrate to nearly dry, nitrogen Blow to dryness, redissolve with 2mL acetonitrile for later use;

[0032] (3) Column separation: wash the HLB column with 5mL ethyl acetate, then wash the column with 5ml acetone, load the sample, rinse the column with 10mL of acetonitrile and water mixture with a volume ratio of 1:2, and finally wash the column with 10ml eluted with acetonitrile, collected the eluent, rotary evaporated to 1ml, blown dry with nitrogen, and dilute to 2ml with acetonitrile for detection;

[0033] (4) Detection: HPLC is...

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PUM

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Abstract

The invention discloses a method for detecting residual metalaxyl in eggplant, which comprises the following steps: 1)processing a sample: placing the picked eggplant in a crusher for crushing to obtain eggplant mud; 2)extracting: weighing 20g eggplant mud in an erlenmeyer flask, adding 100mL mixed liquor of ethyl acetate and acetone, oscillating and extracting for 60-70 minutes at 35-40 DEG C, performing pumping filtration to obtain a supernate, concentrating until almost dry, drying by nitrogen, using 2mL acetonitrile to redissolve for standby; 3)performing column separation: using 5mL ethyl acetate for washing HLB column, using 5mL acetone for washing column, loading a sample, using 10mL mixed liquor of acetonitrile and water with volume ratio being 1:2 for leaching the column, finally using 10ml acetonitrile for eluting, collecting an eluate, performing rotary evaporateion to 1ml, drying by nitrogen, employing acetonitrile for metering volume to 2ml, waiting for detecting; and 4)employing HPLC for detecting.

Description

technical field [0001] The invention relates to a method for detecting metalaxyl residues in eggplant, and belongs to the technical field of pesticide residue detection. Background technique [0002] Metalaxyl is a low toxicity fungicide. The acute oral LD50 of the original drug in rats was 669 mg / kg, and the acute percutaneous LD50>3100 ml / kg. Slightly irritating to eyes and skin, low toxicity to fish, TLM of trout is 100mg / kg (96h). Metalaxyl belongs to the phenylamide family of high-efficiency, low-toxicity, low-residue, systemic fungicidal pesticides. Its systemic and penetrating power is very strong, and it can conduct bidirectionally up and down in the plant 30 minutes after application. It has protective and therapeutic effects on diseased plants, and the drug effect lasts for a long time. It mainly inhibits the synthesis of proteins in the mycelium of pathogenic bacteria, making It lacks nutrients, cannot grow normally and dies. It is effective against downy m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 徐晓华张雷
Owner SUZHOU APP SCI ACAD CO LTD