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Silage processing method of switchgrass

A processing method and technology of willow branches, applied in the field of agriculture, can solve problems such as unsatisfactory results, and achieve the effects of improving fermentation level, low cost, and low pH value

Inactive Publication Date: 2015-04-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the different sources and doses of the selected bacteria and enzymes, the substrates are also different, so the effect of using this kind of additives in the process of switchgrass silage is not ideal.

Method used

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  • Silage processing method of switchgrass
  • Silage processing method of switchgrass

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Experimental program
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Effect test

Embodiment 1

[0023] The preparation of embodiment 1 composite bacterial agent

[0024] Inoculate Lactobacillus plantarum, Bacillus subtilis, and Lactococcus lactis into MRS-S medium respectively, and culture them statically at 30°C for 72 hours. The ratio of the number of forming units is 94:4.5:1.5, and the total number of colony forming units is 1×10 12 CFU / ml, the silage inoculant is obtained.

Embodiment 2

[0025] Embodiment 2 switchgrass silage processing method

[0026] Proceed as follows:

[0027] Mix the composite bacterial agent obtained in Example 1 with cellulose complex enzyme C9748, add a small amount of water to evenly sandblast the surface of the switchgrass, and wherein the composite bacterial agent is added in an amount of 5.2×10 7 CFU / gFM, the amount of cellulose compound enzyme added is 8mg / Kg DM; put the switchgrass into a 2L screw bottle, compact and seal it, and carry out anaerobic fermentation for 30 days.

Embodiment 3

[0028] Embodiment 3 switchgrass silage processing method

[0029] Ferment according to the silage processing method described in embodiment 2, difference is:

[0030] 1) The cellulose complex enzyme is disintegrating enzyme D9515, CAS No. 85186-71-6, derived from Basidiomycetes sp., purchased from Sigma-Aldrich;

[0031] 2) The amount of compound bacterial agent added is 4.5×10 7 CFU / g FM, the added amount of cellulose compound enzyme is 10mg / Kg DM.

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Abstract

The invention relates to a silage processing method of switchgrass. The method comprises the following steps: simultaneously adding a complex microbial inoculant and cellulose complex enzyme to the switchgrass and carrying out anaerobic fermentation, wherein the complex microbial inoculant is prepared by respectively fermenting lactobacillus plantarum, bacillus subtilis and galactococcus to obtain fermentation liquors, and mixing the obtained fermentation liquors. The cellulose complex enzyme is selected from one or more of driselase D9515, cellulose V2010, cellulose C9748 or hemicellulase. According to the silage processing method provided by the invention, the fermentation level for switchgrass silage is effectively improved; a fermented product is low in pH value, and high in coarse protein content and in-vitro dry matter digestibility; the content uniformity of a neutral detergent fiber, the cellulose, hemicellulose and lignin is reduced to a certain extent; the silage processing method provided by the invention is ecological, safe, simple and easy to apply, and low in cost; and the silage effect is obviously improved.

Description

technical field [0001] The invention relates to a method for processing switchgrass silage, which belongs to the technical field of agriculture. Background technique [0002] In recent years, with the development of my country's animal husbandry, the rapid development of grassland forage industry has been driven. High-quality grass forage is more and more favored by people. However, as the number of livestock continues to increase, the natural growth of these pastures can no longer meet the nutritional needs of livestock throughout the year. Therefore, producers solve this contradiction by artificially planting high-quality and high-yield pastures. Gramineous forages such as switchgrass have high productivity, excellent forage characteristics and soil and water conservation capabilities, and strong stress resistance. They can be planted in arid and semi-arid areas, low-lying areas prone to waterlogging and saline-alkali, and mountainous and semi-mountainous areas with poor ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K3/03
Inventor 王小芬崔宗均刘晶晶袁旭峰朱万斌
Owner CHINA AGRI UNIV
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