Microbial agent as well as preparation method and application thereof

A technology for microbial inoculants and bacilli, applied in the field of microbial inoculants and their preparation, can solve the problems of low plant growth index and the like

Inactive Publication Date: 2015-04-29
BEIJING JUNO SCI AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problem of low plant strong seedling index existing in the current vegetable seedling raising process, to provide a microbial agent capable of improving the plant strong seedling index and its preparation method and application

Method used

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  • Microbial agent as well as preparation method and application thereof

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preparation example Construction

[0014] The present invention also provides a preparation method of the microbial agent, wherein the method comprises: inoculating Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus safensis in the culture medium To cultivate.

[0015] According to the present invention, the cultivation method includes: respectively cultivating Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus safensis in separate culture systems, and culturing them separately to obtain The microorganisms are mixed in proportion. Preferably, by cultivating in separate culture systems and mixing after culturing, the total number of live bacteria per gram of microbial agent obtained is 2-10×10 7 indivual.

[0016] In a preferred embodiment, by cultivating in separate culture systems and mixing in proportion after culturing, the mixing conditions are not particularly limited, as long as the obtained microbial inoculum can meet the following conditions: Based on the total number of viable bacter...

Embodiment 1

[0025] (1) Inoculate 5 parts by weight of Bacillus subtilis (ACCC01266) in 100 parts by weight of medium (glucose 5g, soybean meal 30g, peptone 2g, distilled water 1000ml, PH7.0-7.2), culture at 37°C, during the culture process Sampling was carried out and observed by microscope direct counting until the viable count of Bacillus subtilis was 2×10 7 per gram of culture medium.

[0026] (2) In 100 parts by weight of the medium (50g bean cake powder, 20g sucrose, 5g ammonium sulfate, 2.5g trisodium citrate, 0.3g potassium dihydrogen phosphate, 0.5g magnesium sulfate, 0.05g ferrous sulfate, pH7.0 -7.2) Inoculate 4 parts by weight of Bacillus amyloliquefaciens (ACCC1855), culture at 37°C, take samples during the cultivation and observe by direct counting under microscope until the number of viable bacteria of Bacillus amyloliquefaciens reaches 2×10 7 per gram of culture medium.

[0027] (3) In 100 parts by weight of medium (glucose 15g, starch 1g, bean cake powder 25g, manganese ...

Embodiment 2

[0030] (1) Inoculate 4 parts by weight of Bacillus subtilis (ACCC01266) in 100 parts by weight of medium (glucose 5g, soybean meal 30g, peptone 2g, distilled water 1000ml, pH7.0-7.2), and culture at 37°C. Sampling was carried out and observed by microscope direct counting method until the viable count of Bacillus subtilis was 8×10 7 per gram of culture medium.

[0031] (2) In 100 parts by weight of the medium (50g bean cake powder, 20g sucrose, 5g ammonium sulfate, 2.5g trisodium citrate, 0.3g potassium dihydrogen phosphate, 0.5g magnesium sulfate, 0.05g ferrous sulfate, pH7.0 -7.2) was inoculated with 3 parts by weight of Bacillus amyloliquefaciens (ACCC1855), cultured at 37°C, samples were taken during the cultivation and observed by direct counting under a microscope until the number of viable Bacillus amyloliquefaciens was 8×10 7 per gram of culture medium.

[0032] (3) In 100 parts by weight of medium (glucose 15g, starch 1g, bean cake powder 25g, manganese sulfate 1g, ...

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Abstract

The invention provides a microbial agent. The microbial agent comprises a culture medium and thalli, wherein the thalli comprise Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus safensis. The microbial agent provided by the invention can obtain a good plant seedling index and further improve yield and quality of vegetables, thereby having broad application prospects in the field of vegetable seedling raising. In addition, by using the microbial agent provided by the invention, a plant rhizosphere microbial environment can be improved, and beneficial bacteria are increased, thereby inhibiting the growth of harmful bacteria and further improving the disease resistance of plants.

Description

technical field [0001] The present invention relates to a microbial bacterial agent and its preparation method and application; more specifically, it relates to a microbial bacterial agent and its preparation method, and also relates to the application of the biological bacterial agent in vegetable seedling cultivation. Background technique [0002] With the development of the world's agricultural economy, the agricultural planting structure is also undergoing corresponding adjustments, gradually transforming from traditional agriculture to modern agriculture, and the planting area of ​​vegetables is also increasing year by year, and seedling cultivation is a very important technical link in vegetable cultivation , Vegetable seedlings can not only make vegetables appear on the market earlier, but also increase vegetable yield and improve quality. [0003] At present, the problems in vegetable seedling cultivation are serious soil-borne diseases in vegetable seedling cultivat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125C12R1/07
CPCC12N1/20A01N63/00
Inventor 高红春朱昌雄顾金刚周艳丽
Owner BEIJING JUNO SCI AGRI TECH
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