Detection method for cation exchange capacity in culture medium
A technology of cation exchange capacity and cultivation substrate, which is applied in the field of detection of cation exchange capacity in cultivation substrate, and can solve problems such as inability to effectively separate by centrifugation, small specific gravity of cultivation substrate, and light texture
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[0024] 1. Main instruments and reagents
[0025] K9840 automatic Kjeldahl nitrogen analyzer; LG10-2.4A high-speed centrifuge.
[0026] Reagents: ammonium acetate, acetic acid, ethanol, boric acid, concentrated hydrochloric acid, sodium hydroxide, methyl red, bromocresol green, etc., all of analytical grade.
[0027] Nylon filter cloth: 200 mesh.
[0028] 2. Reagent solution
[0029] 2.11moL / L ammonium acetate solution (pH 7.0): 77.09g of ammonium acetate was dissolved in water and diluted to nearly 1L. Measure the pH, if the pH is not at 7.0, adjust to 7.0 with 1:1 ammonia water or dilute acetic acid, then dilute to 1L.
[0030] 2.295% ethanol solution (without ammonium ions).
[0031] 2.3 Liquid paraffin.
[0032] 2.4 Methyl red-bromocresol green mixed indicator: 0.099g bromocresol green and 0.066g methyl red in an agate mortar, add a small amount of 95% ethanol, grind until the indicator is completely dissolved, and finally add 95% ethanol to 100mL.
[0033] 2.52% bor...
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