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Detection method for cation exchange capacity in culture medium

A technology of cation exchange capacity and cultivation substrate, which is applied in the field of detection of cation exchange capacity in cultivation substrate, and can solve problems such as inability to effectively separate by centrifugation, small specific gravity of cultivation substrate, and light texture

Inactive Publication Date: 2015-04-29
青岛谱尼测试有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are problems in the method of measuring the cation exchange capacity of the cultivation substrate. The specific gravity of the cultivation substrate is small and the texture is light, so it cannot be effectively separated by centrifugation; at present, there is no specific method for the detection of the cation exchange capacity of the cultivation substrate.

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  • Detection method for cation exchange capacity in culture medium
  • Detection method for cation exchange capacity in culture medium
  • Detection method for cation exchange capacity in culture medium

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Embodiment 1

[0024] 1. Main instruments and reagents

[0025] K9840 automatic Kjeldahl nitrogen analyzer; LG10-2.4A high-speed centrifuge.

[0026] Reagents: ammonium acetate, acetic acid, ethanol, boric acid, concentrated hydrochloric acid, sodium hydroxide, methyl red, bromocresol green, etc., all of analytical grade.

[0027] Nylon filter cloth: 200 mesh.

[0028] 2. Reagent solution

[0029] 2.11moL / L ammonium acetate solution (pH 7.0): 77.09g of ammonium acetate was dissolved in water and diluted to nearly 1L. Measure the pH, if the pH is not at 7.0, adjust to 7.0 with 1:1 ammonia water or dilute acetic acid, then dilute to 1L.

[0030] 2.295% ethanol solution (without ammonium ions).

[0031] 2.3 Liquid paraffin.

[0032] 2.4 Methyl red-bromocresol green mixed indicator: 0.099g bromocresol green and 0.066g methyl red in an agate mortar, add a small amount of 95% ethanol, grind until the indicator is completely dissolved, and finally add 95% ethanol to 100mL.

[0033] 2.52% bor...

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Abstract

The invention belongs to the technical field of soil detection, particularly relates to a detection method for cation exchange capacity in a culture medium, and discloses a determination method for the cation exchange capacity of the culture medium. The determination method comprises the following steps: designing a centrifugal inner net tube, namely cutting a rectangle of which the area is 10.0cm*12.0cm and a circle of which the diameter is 4cm and the area is 12.6cm<2> from 200-mesh filter cloth, sewing the cut rectangular filter cloth and the circular filter cloth into cylindrical filter cloth by using a needle and threads, putting the cylindrical filter cloth shown in a figure into a centrifugal tube of which the volume is 50mL, weighing an appropriate quantity of a culture medium, tightly tying an opening of the cylindrical filter cloth by using a string, pouring ammonium acetate and ethyl alcohol, and starting centrifugation; after the centrifugation, directly extracting the cylindrical filter cloth and a sample, then putting the cylindrical filter cloth and the sample into a distilling flask of an azotometer, and starting distillation. The method is simple and easy to operate, and a determination result is accurate.

Description

technical field [0001] The invention belongs to the technical field of soil detection, and in particular relates to a method for detecting cation exchange capacity in a cultivation substrate. technical background [0002] The ability of soil to adsorb cations is expressed by the total amount of adsorbed cations, which is called the cation exchange capacity, and its value is expressed in centimoles per kilogram (cmol(+)kg -1 )express. The cation exchange capacity can be used as an index to evaluate soil fertility. It is the main source of soil buffer performance and an important basis for improving soil and rational fertilization. Therefore, the cation exchange capacity, which is an important index reflecting the total negative charge of soil and characterizing soil properties, is very important. The determination of cation exchange capacity is affected by many factors. For example, the nature of the exchanger, the concentration and pH of the salt solution, etc., must be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/16G01N21/79G01N1/28
Inventor 张英杰宋薇
Owner 青岛谱尼测试有限公司
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