A method for removing heavy metal cadmium from rice by fermenting compound lactic acid bacteria
A technology of lactic acid bacteria fermentation and heavy metals, which is applied in food science and other fields, can solve the problems of reducing the protein and ash content of rice flour, achieve high removal rate, significant social and economic benefits, and simple and reliable process
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Embodiment 1
[0016] Take 10 g of rice samples with a cadmium content of 0.6479 mg / kg, crush them through a 40-mesh sieve, add 40 mL of deionized water, and mix to make a rice slurry; and 37°C for 24 hours to activate the strains; put the activated single colonies of slanted strains of Lactobacillus plantarum and Pediococcus pentosaceus into the sterilized MRS broth medium respectively, at 42°C and 37°C respectively Cultivate at constant temperature for 48 hours, respectively prepare Lactobacillus plantarum seed culture solution and Pediococcus pentosacea seed culture solution; mix Lactobacillus plantarum seed culture solution and Pediococcus pentosacea seed culture solution at a ratio of 2:1 (v / v) Mix, add 3% (v / v) inoculum into the rice slurry, and ferment at a constant temperature of 40°C for 24 hours; after the fermentation is completed, filter the fermentation liquid to obtain fermented rice flour, which is fermented 3-5 times Wash the fermented rice flour 3 times with deionized water ...
Embodiment 2
[0022] Take 10 g of rice samples with a cadmium content of 0.4783 mg / kg, crush them through a 50-mesh sieve, add 50 mL of deionized water, and mix to make a rice slurry; and 37°C for 24h to activate the strains; the activated single colonies of the slanted strains of Lactobacillus plantarum and Pediococcus pentosacea were respectively inserted into the sterilized MRS broth medium, and the bacteria were incubated at 37°C and 42°C respectively. Cultivate at constant temperature for 24 hours to prepare Lactobacillus plantarum seed culture solution and Pediococcus pentosacea seed culture solution respectively; the ratio of Lactobacillus plantarum seed culture solution and Pediococcus pentosacea seed culture solution is 2:1 (v / v) Mix, add 5% (v / v) inoculum into the rice slurry, and ferment at a constant temperature of 43°C for 18 hours; after the fermentation is complete, filter the fermentation liquid to obtain fermented rice flour, which is fermented 3-5 times Wash the fermented ...
Embodiment 3
[0024] Take 10 g of rice samples with a cadmium content of 0.8095 mg / kg, crush them through a 60-mesh sieve, add 60 mL of deionized water, and mix to make a rice slurry; and 37°C for 24h to activate the strains; put the activated Lactobacillus plantarum and Pediococcus pentosaceae slant strain single colonies into the sterilized MRS broth medium, respectively, at 40°C and 39°C Constant temperature static culture for 36 hours, respectively prepared Lactobacillus plantarum seed culture solution and Pediococcus pentosacea seed culture solution; the ratio of Lactobacillus plantarum seed culture solution and Pediococcus pentosacea seed culture solution was 2:1 (v / v) Mix, insert into rice slurry according to 4% (v / v) inoculum amount, and ferment for 30 hours at a constant temperature at 37°C; after the fermentation is completed, filter the fermentation liquid to obtain fermented rice flour, and use 3-5 times of fermentation Wash the fermented rice flour 4 times with deionized water ...
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