Application of Monascus Pigment Components and Its Derivatives in the Preparation of Anticancer Photosensitizers
A technology of monascus pigments and derivatives, which is applied in the application field of monascus pigment components and their derivatives in the preparation of anticancer photosensitizers, which can solve the problem of unsatisfactory action spectrum, complex active ingredients, and large skin phototoxicity and side effects. problems, to achieve the effect of expanding the application field, significant growth, and small toxic and side effects
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Embodiment 1
[0037] Preparation of monascus pigment crude extract:
[0038] Steam the rice until it is fully cooked. After cooling, it is inoculated with Monascus and placed in solid state fermentation at 30℃ for 8 days. 505nm), containing red pigments (erythroscine and monascorubin) 10.1 g / kg, orange pigments (erythematine and monascorubin) 48.3 g / kg, yellow pigments (monascoxine and monascus) 41.9 g / kg.
[0039] The fermented product is extracted with a 70% mass fraction of ethanol solution at a solid-liquid ratio of 1:10 in a 60°C water bath, and then concentrated in a vacuum to 1 / 2 of the original volume, that is, a crude extract of the monascus pigment component is precipitated with a yield of 10.1% , The pigment component accounts for 78.9% of the total weight of the crude extract.
[0040] Take two 96-well plates as the non-light group and the light group respectively, and culture the Hela cells in the logarithmic growth phase in two 96-well plates. After the cells are fully attached an...
Embodiment 2
[0042] Purification of monascus pigment components:
[0043] Purification of monascus pigment components: the crude monascus pigment components prepared in Example 1 were separated and purified by HPLC (C18 column), and 80% methanol solution was used as the eluent to collect monascus and monascus respectively The eluted samples of ruberin and lupusamine components were concentrated in a vacuum to 1 / 2 of the original volume to precipitate crystals. The obtained crystals of monascin, monascin and lupusamine components were determined by HPLC-MS, and their molecular weights were respectively 358, 382 and 353, the purity is 99.3%, 98.6% and 98.9% respectively.
[0044] Take 3 96-well plates and set them as the monascin-illuminated group, the monascorubin-illuminated group, and the lupusamine-illuminated group, and culture the Hela cells in the logarithmic growth phase in three 96-well plates, and wait until the cells are fully attached After growth, add equivalent amounts of monascin,...
Embodiment 3
[0046] Preparation of monascorubin derivative N-glutaryl monascorubamine: 1 g of monascorubin prepared in Example 2 was dissolved in 100 mL ethanol solution with a mass fraction of 70%, and placed in a water bath at 60 ℃ Add 10 mL of monosodium glutamate (1mol / L), and after reacting for 1 hour, use a silica gel column for purification, elution with anhydrous methanol, collect the eluted sample of N-glutaryl monascus red amine, and concentrate in vacuo to the original volume 1 / 2 means that the crystals are precipitated, and the N-glutaryl monascinolamine crystals are determined by HPLC-MS. The molecular weight is 511 and the purity is 99.1%.
[0047] Preparation of 4-hydroxyerythenamine derivative of Erythenamine: 1 g of Erythenamine prepared in Example 2 was dissolved in 100 mL of ethanol with a mass fraction of 70%, and 5 mL of sodium borohydride (1 mol) was added in a water bath at 30 ℃. / L), after reacting for 1 hour, use silica gel column purification, elution with anhydrous ...
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