Outer membrane vesicles

An outer membrane vesicle, heterologous protein technology, applied in allergic diseases, antibody medical ingredients, antibacterial drugs, etc., can solve the problems of no excess, no function, difficult periplasmic protein targeting to OMV, etc.
CN104602702AActive Publication Date: 2015-05-06GLAXOSMITHKLINE BIOLOGICALS SA
27 Cites 15 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GLAXOSMITHKLINE BIOLOGICALS SA
Publication Date
2015-05-06

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention provides an outer membrane vesicle (OMV) from a Gram-negative bacterium, comprising at least one heterologous protein that is free in the lumen of the OMV, wherein the OMV is capable of eliciting an immune response to the heterologous protein when administered to a mammal. The invention also provides methods for preparing the OMVs of the invention, pharmaceutical compositions comprising the OMVs of the invention, especially immunogenic compositions and vaccines, and methods of generating an antibody immune response in a mammal using OMVs.
Need to check novelty before this filing date? Find Prior Art

Description

[0001] This application claims the benefit of U.S. Provisional Application No. 61 / 702,296, filed September 18, 2012, and U.S. Provisional Application No. 61 / 799,311, filed March 15, 2013, the entire contents of which are incorporated herein by reference for all purposes . technical field

[0002] The present invention relates to vesicles from Gram-negative bacteria. Vesicles contain heterologous proteins present in their lumen. The vesicles are particularly useful in immunogenic compositions, such as vaccines. Background technique

[0003] Gram-negative bacteria can spontaneously release outer membrane vesicles (OMVs) during their growth due to the turgor of the cell envelope. Formation of such OMVs can be facilitated by the disruption of certain bacterial components, eg in refs 1 and 2 the E. coli Tol-Pal system was disrupted to obtain strains that release vesicles into the medium during growth. OMVs can also be produced by destroying intact bacteria. Known methods for ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More