Process for extracting total triterpenes from silverweed cinquefoil roots

Abstract

The invention relates to a process for extracting total triterpenes from silverweed cinquefoil roots. The process comprises the following steps: (1) pulverizing the silverweed cinquefoil roots, and sieving to obtain silverweed cinquefoil root powder; (2) extracting the silverweed cinquefoil root powder with the solution of ethanol to obtain an extract, filtering the extract, and concentrating by reducing pressure to obtain an concrete; (3) adding water to precipitate the concrete, stirring, standing at room temperature, and filtering to obtain a precipitate; (4) adding ethanol absolute to dissolve the precipitate to obtain a solution of dissolution, adding activated carbon into the solution of dissolution to decolor and remove impurities, recovering ethanol, and drying the solution of dissolution to constant weight by reducing pressure to obtain a crude extract; (5) dissolving the crude extract, adding ethyl ether to extract, standing at room temperature to layer, extracting repeatedly for 3-5 times, and mixing extracts to obtain an extract; (6) adding n-butanol into the extract to extract, standing at room temperature to layer to obtain the solution of n-butanol, extracting repeatedly for 4-7 times, mixing extracts to obtain the solution of n-butanol, and drying the solution of n-butanol to constant weight by reducing pressure to obtain the total triterpenes from the silverweed cinquefoil roots. The process provided by the invention is simple and efficient and is low in cost.

Description

technical field [0001] The invention relates to a process for extracting total triterpenes, in particular to a process for extracting total triterpenes of Bracken. Background technique [0002] The scientific name of Potentilla velvet is Potentilla velvet, a perennial herb belonging to the Rosaceae Potentilla genus. "Potentilla velvet" generally refers to the enlarged root tubers of Potentilla velvet, one of the more commonly used varieties of Tibetan medicine in my country. Root tubers have the effects of promoting body fluid and relieving cough, invigorating the spleen and stomach, astringent and hemostasis, etc. The triterpenoids contained in the root tubers are mainly triterpene acids and triterpene glycosides, which have pharmacological activities such as anti-inflammatory, anti-tumor, antibacterial, and immune regulation . At present, it has been applied in the field of medicine. However, the current extraction process of total triterpenes has cumbersome steps and do...

AI Technical Summary

Problems solved by technology

However, the current extraction process of total triterpenes is cumbersome and does not involve the removal of starch and sugar

Due to the low content of total triterpenes and high content of starch and sugar in Bracken, a large amount of starch and sugar will be extracted during the extraction process, which will cause great trouble to the purification of total triterpenes in the later stage.