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Pathological tissue transparentizing and dewaxing solution and preparation method thereof

A dewaxing liquid and pathological technology, applied in the field of pathological tissue transparent dewaxing liquid and its preparation, can solve problems such as unfavorable sectioning, irritating odor, toxicity, etc., and achieve the effect of being beneficial to sectioning and enhancing the effect of wax dipping

Active Publication Date: 2015-05-27
无锡市江原实业技贸有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention is that in the prior art, the transparent dewaxing liquid of pathological tissue is poisonous and has irritating smell, and soaking tissue for a long time can make the tissue brittle and shrink unfavorable for slicing, and then provide a nontoxic, non-toxic Transparent dewaxing solution for pathological tissue with pungent smell and soaking tissue for a long time without making the tissue brittle and shrinking, and its preparation method and application

Method used

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  • Pathological tissue transparentizing and dewaxing solution and preparation method thereof
  • Pathological tissue transparentizing and dewaxing solution and preparation method thereof
  • Pathological tissue transparentizing and dewaxing solution and preparation method thereof

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Effect test

Embodiment 1

[0049] The pathological tissue transparent dewaxing liquid of the present embodiment comprises:

[0050] Naphthenic oil 92.5kg;

[0051] Trimethylolpropane 5kg;

[0052] Dimethyl-3-hydroxypropylmethyl (siloxane and polysiloxane) 0.1kg;

[0053] The above-mentioned highly branched saturated alkanes, stabilizers and synergists are blended at room temperature to 60° C. to form the transparent dewaxing solution for pathological tissues.

[0054] Take the pathological tissue transparent dewaxing solution prepared above to carry out the first transparent, dewaxed and second transparent steps on the pathological tissue: the first transparent step is: put the pathological tissue after alcohol dehydration into the In the pathological tissue transparent dewaxing solution, soak at a constant temperature of 32°C for 45 minutes, and repeat the soaking once; the dewaxing step is specifically: put the processed pathological tissue paraffin sections into the pathological tissue transparent ...

Embodiment 2

[0056] The pathological tissue transparent dewaxing liquid of the present embodiment comprises:

[0057] Tetracycloheptane 98kg;

[0058] Epoxidized soybean oil 0.5kg;

[0059] Polyether modified heptamethyltrisiloxane 2.5kg.

[0060] The above-mentioned highly branched saturated alkanes, stabilizers and synergists are blended at room temperature to 60° C. to form the transparent dewaxing solution for pathological tissues.

[0061] Take the pathological tissue transparent dewaxing solution prepared above to carry out the first transparent, dewaxed and second transparent steps to the pathological tissue: the first transparent step is: put the pathological tissue after alcohol dehydration into the In the pathological tissue transparent dewaxing solution, soak at a constant temperature of 37°C for 45 minutes, and repeat the soaking once; the dewaxing step is specifically: put the processed pathological tissue paraffin sections into the pathological tissue transparent dewaxing s...

Embodiment 3

[0063] The pathological tissue transparent dewaxing liquid of the present embodiment comprises:

[0064] Cis-1-isopropyl-4-methylcyclohexane 95kg;

[0065] Epoxy butyl stearate 3kg;

[0066] Butyl ether polydimethylsiloxane 1kg.

[0067] The above-mentioned highly branched saturated alkanes, stabilizers and synergists are blended at room temperature to 60° C. to form the transparent dewaxing solution for pathological tissues.

[0068] Take the pathological tissue transparent dewaxing solution prepared above to carry out the first transparent, dewaxed and second transparent steps on the pathological tissue: the first transparent step is: put the pathological tissue after alcohol dehydration into the In the above pathological tissue transparent dewaxing solution, soak at a constant temperature of 34°C for 50 minutes, and repeat the soaking once; In the wax solution, soak at a constant temperature at 24°C for 13 minutes, and repeat the soaking twice; the second transparent ste...

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Abstract

The invention discloses a pathological tissue transparentizing and dewaxing solution and a preparation method thereof. The pathological tissue transparentizing and dewaxing solution comprises 92.5-98 parts by weight of highly branch chain saturated alkane, 0.5-5 parts by weight of a stabilizing agent and 0.1-2.5 parts by weight of synergist, wherein the highly branch chain saturated alkane is one or a mixture of more of naphthenic oil, quadricyclane, cis-1-isopropyl-4-methyl cyclohexane, and contra-1-isopropyl-4-methyl cyclohexane; the stabilizing agent is one or a mixture of more of epoxy soybean oil, trimethylolpropane, and epoxy butyl stearate; the synergist is one or a mixture of more of polyether modified heptamethyltrisiloxane, butyl ether polydimethylsiloxane, and dimethyl-3-hydroxypropyl methyl (siloxane and polysiloxane). The technical problems that a pathological tissue transparentizing and dewaxing solution is toxic and has pungent smell, and tissue becomes brittle and shrinks to be unfavorable for slicing after being soaked in the solution for a long time in the prior art are solved.

Description

technical field [0001] The invention belongs to the technical field of pathological tissue slices, and in particular relates to a transparent dewaxing liquid for pathological tissue and its preparation method and application. Background technique [0002] At present, the production process of most pathological tissue sections still adopts the traditional paraffin section production technology. The processing of steps can complete the making of pathological slides. Among them, the first time of transparency refers to the purpose of immersing paraffin into the tissue block through the mediation of the transparent liquid. During this process, the hydrophilic components in the tissue block are replaced by the transparent liquid, making the tissue block transparent; and Dewaxing refers to the operation of dissolving the paraffin in the tissue section with dewaxing solution before staining, so that the dye is easy to immerse in the tissue and cells; the second transparent refers ...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
Inventor 谢敏浩吴军戴军邹霈何拥军刘娅灵王洪勇吴昊
Owner 无锡市江原实业技贸有限公司
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