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Method for tissue culture and rapid propagation of acer palmatum hogyoku

A technology of tissue culture rapid propagation and Baoyu, which is applied in the field of plant tissue culture to achieve the effects of simple formula, low cost of medium, and simple culture process

Active Publication Date: 2015-06-03
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on the tissue culture of Baoyu. Therefore, it is urgent to study the rapid propagation technology of Baoyu tissue culture and establish a tissue culture rapid propagation system of Baoyu to obtain sterile seedlings of Baoyu.

Method used

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  • Method for tissue culture and rapid propagation of acer palmatum hogyoku
  • Method for tissue culture and rapid propagation of acer palmatum hogyoku
  • Method for tissue culture and rapid propagation of acer palmatum hogyoku

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1: A kind of tissue culture rapid propagation method of Baoyu, it comprises the following steps:

[0022] (1) Sterilization of explants: Take Baoyu’s young shoots that are free from diseases and insect pests, remove the leaves, cut the stems into 2cm bud stems, place them on an ultra-clean workbench, and sterilize them with 75% alcohol for 6 seconds. Sterilize with 0.1% mercuric chloride for 10 minutes, rinse with sterile water for 6 times;

[0023] (2) Start-up culture: inoculate the sterilized budded stems into the start-up medium, and cultivate them in a culture room with a light intensity of 2000LX, a light time of 18h / d, and a temperature of 24°C for 2 weeks, and the axillary buds will elongate on average. 2cm, wherein, the starting medium is: B6+0.1mg / LIAA+0.05mg / LTDZ+sucrose 30g / L+agar 5g / L, pH 6.3;

[0024] (3) Proliferation culture: cut out the axillary buds and inoculate them into the proliferation medium, keep the conditions in the culture room unc...

Embodiment 2

[0026] Example 2: A kind of tissue culture rapid propagation method of Baoyu, it comprises the following steps:

[0027] (1) Sterilization of explants: Take Baoyu’s young shoots that are free from diseases and insect pests, remove the leaves, cut the stems into 3cm bud stems, place them on an ultra-clean workbench, and sterilize them with 75% alcohol for 10 seconds. Sterilize with 0.1% mercuric chloride for 6 minutes, rinse with sterile water for 4 times;

[0028] (2) Start-up culture: inoculate the sterilized bud-bearing stems into the start-up medium, and cultivate in a culture room with a light intensity of 3000LX, a light time of 14h / d, and a temperature of 28°C for 3 weeks, and the axillary buds will elongate on average 4cm, where the starting medium is: B6+0.1mg / LIAA+0.05mg / LTDZ+sucrose 30g / L+agar 5g / L, pH 6.7;

[0029] (3) Proliferation culture: cut out the axillary buds and inoculate them into the proliferation medium, keep the conditions in the culture room unchang...

Embodiment 3

[0031] Example 3: A kind of tissue culture rapid propagation method of Baoyu, it comprises the following steps:

[0032] (1) Sterilization of explants: Take Baoyu's young shoots that are free from diseases and insect pests, remove the leaves, cut the stems into 3cm bud stems, place them on a super-clean workbench, and sterilize them with 75% alcohol for 7s. Sterilize with 0.1% mercuric chloride for 8 minutes, rinse with sterile water for 5 times;

[0033] (2) Start-up culture: inoculate the sterilized bud-bearing stems into the start-up medium, and cultivate in a culture room with a light intensity of 2300LX, a light time of 16h / d, and a temperature of 25°C for 16 days, and the axillary buds will elongate on average 2.8cm, wherein, the starting medium is: B6+0.1mg / LIAA+0.05mg / LTDZ+sucrose 30g / L+agar 5g / L, pH 6.5;

[0034] (3) Proliferation culture: cut off the axillary buds and inoculate them into the proliferation medium, keep the conditions in the culture room unchanged, ...

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Abstract

The invention discloses a method for tissue culture and rapid propagation of acer palmatum hogyoku, belonging to the technical field of plant tissue culture. The method for the tissue culture and rapid propagation of the acer palmatum hogyoku comprises the following steps: sterilizing an explant, and carrying out culture, proliferation culture and rooting culture. The method for the tissue culture and rapid propagation of the acer palmatum hogyoku has the advantages that a tissue culture and rapid propagation system is established, a used culture medium adopts a simple formula, the culture medium is low in cost, a culture process is simple and convenient, the acer palmatum hogyoku propagation coefficient is high, and technical support is provided for industrial seedling culture and deep processing of the acer palmatum hogyoku.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a tissue culture method, in particular to a tissue culture rapid propagation method of Baoyu. Background technique [0002] Baoyu ( Acer palmatum 'Hogyoku' ) is a variety of Acer palmatum in the Acer family Acer family. It has a beautiful tree shape, bright dark orange leaves in autumn, and darker bright green leaves in spring and summer. Baoyu plant is strong, strong resistance, dense branches, fast growth, mature plants up to 6m, easy to trim shape. Baoyu is a new species of Acer palmatum with better visual effects. Its dark green color and unique orange color of autumn leaves are very attractive and have high ornamental value. landscape etc. However, the number of precious jade is small, and grafting is generally used for propagation. Its growth cycle is long, cuttings are not easy to take root, the propagation speed is slow, and the reproduction rate is low. It ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 沈香兰马建华王小辉朱晓菲马良良杨小霞
Owner SICHUAN COLORLINK CO LTD
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