Nasal immune vaccine for porcine epizootic diarrhea virus and preparation method of immune vaccine

A porcine epidemic diarrhea and immunization vaccine technology, which is applied in the field of porcine epidemic diarrhea virus nasal mucosa immunization vaccine and its preparation, achieves high potency, prevents the harm of piglet epidemic diarrhea, and has good immune effect

Active Publication Date: 2015-06-03
GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no effective mucosa...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nasal immune vaccine for porcine epizootic diarrhea virus and preparation method of immune vaccine
  • Nasal immune vaccine for porcine epizootic diarrhea virus and preparation method of immune vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 vaccine

[0025] Porcine epidemic diarrhea virus CHYJ130330 infected Vero cells (African green monkey kidney cells), cultured, harvested virus liquid, and determined TCID 50 1. After inactivation, the vaccine stock solution is obtained, which is used for further preparation of vaccines. The specific operation steps are as follows:

[0026] 1) Preparation of vaccine stock solution

[0027] Virus culture: Resuscitate Vero cells, after expansion, until the cells grow into a thin monolayer, inoculate the PEDV virus CHYJ130330 into the cell bottle according to the volume ratio (1:10) ~ (1:100), at 37 ° C, 5% CO 2 Cultivate in an incubator, and observe the cytopathic changes every day. When the cytopathic changes reach more than 80%, the virus liquid is harvested and stored at -20°C for later use.

[0028] Virus inactivation: measure TCID before virus liquid inactivation 50 (half the infection amount of the tissue), inactivate the above ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a nasal immune vaccine for a porcine epizootic diarrhea virus (PEDV) and a preparation method of the nasal immune vaccine. According to the preparation method, the nasal immune vaccine is prepared by mixing a vaccine concentrate for the porcine epizootic diarrhea virus, an adjuvant solution and a sodium azide solution at a specific ratio, wherein the adjuvant solution contains a TLR agonist, an agonist for an NOD sample receptor, carbomer and PVPK. Compared with an existing related vaccine, the nasal immune vaccine for the PEDV prepared by the method is high in titer and good in immune effect. The vaccine disclosed by the invention is subjected to vaccine immunization through a nasal mucosa tissue; meanwhile, mucosal immunization and system immunization of the organism are stimulated at the same time; a lot of sIgA is generated in sow milk; and a maternal antibody is transferred to piglets through milk so that the piglets obtain effective immune protection and the harms caused by the epidemic diarrhea of the piglets are prevented.

Description

Technical field [0001] The invention is a veterinary biotechnology field, which involves a pork flow diarrhea virus nasal mucosal immunohic vaccine and its preparation methods. Background technique [0002] PORCINE Epidemic Diarhea Virus (PEDV) can cause pork hair vomiting, diarrhea, and dehydration of pigs of all ages. Pigs in all ages are susceptible, especially for new piglets in 1 week.The incidence of new piglets can reach 60%to 100%, and the mortality rate is as high as 100%.As a result, a large number of piglets died, and the raw pork column rate decreased, resulting in insufficient pork supply in the market, affecting the stability of pork prices.Since December 2010, my country has successively broke out in major pig -raising provinces to explode pig influenza diarrhea. Due to the high incidence and high mortality in the susceptible pig group, it brought a heavy blow to the entire pig industry, causing serious serious blows, causing serious serious blows, causing serious ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K39/215A61K39/39A61K9/00A61P31/14
Inventor 贾爱卿冯晓声王贵平刘琪王悦芸周如月安欣宇
Owner GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap