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Gene for controlling cucumber non-tendril character and cucumber tendril character-related SNP marker

A cucumber and trait technology, applied in the fields of genetic engineering and molecular biology, can solve the problems of slow progress in molecular breeding, unclear genetic positioning and molecular mechanism of cucumber's tendrilless trait, and achieve the effect of speeding up the breeding process

Active Publication Date: 2015-06-03
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditional genetics shows that the tendrilless trait of cucumber is controlled by a pair of recessive genes, but the genetic location and molecular mechanism of the tendrilless trait in cucumber are still unclear, resulting in slow progress in related molecular breeding

Method used

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  • Gene for controlling cucumber non-tendril character and cucumber tendril character-related SNP marker
  • Gene for controlling cucumber non-tendril character and cucumber tendril character-related SNP marker
  • Gene for controlling cucumber non-tendril character and cucumber tendril character-related SNP marker

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 Discovery of SNP sites associated with cucumber tendril traits

[0034] There are 3342 cucumber resources collected from all over the world, and only one cucumber material has no tendrils, which is a rare mutation trait. The present invention has screened 115 cucumber core germplasms from 3342 cucumber resources, and performed resequencing analysis on these 115 cucumber core germplasms. Bioinformatics comparative analysis of the genome sequences of the tendrilless cucumber material and 114 other materials. From 3.6 million SNPs, 1018 rare SNPs unique to the tendrilless cucumber material were screened, and the number of rare SNPs that caused heterosense mutations was 75. Corresponding to 75 genes respectively, combined with the analysis of cucumber transcriptome, a gene Csa5G644520 was found that is only specifically expressed in tendril tissue, and it is speculated that this gene controls the development of cucumber tendrils. The rare SNP on this gene is respo...

Embodiment 2

[0039] Example 2 Initial Genetic Mapping of the Gene Csa5G644520 Controlling the Tendrilless Character of Cucumber

[0040] In order to verify that Csa5G644520 is the gene controlling the development of cucumber tendrils, the method of map-based cloning was used for verification. The F2 population of 6623 individual plants was constructed with a cucumber resource without tendrils (tltl) and a cucumber resource with tendrils (TlTl). Mapping locates the tl gene between the two markers SSR19343 and SSR0618, and the genetic distance is 3.4cM ( figure 2 ).

Embodiment 3

[0041] Example 3 Cloning of the gene controlling the tendril-free trait of cucumber

[0042] In order to clone the cucumber tendrilless gene tl, the two markers SSR19343 and SSR20486 that were initially positioned were selected to screen the remaining F2 population (6437 individual plants), and the Indel marker and SNP marker were developed inwards based on the initial positioning, and detected. The screened recombinant individual plants finally located the tl gene within a physical distance of about 18kb, and there was only one gene Csa5G644520 in this range, that is, the Csa5G644520 gene is the cucumber tendrilless gene tl, and a rare SNP associated with the cucumber tendril phenotype (i.e. SNP1) is located on the exon of the Csa5G644520 gene ( image 3 ).

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Abstract

The invention relates to a gene Csa5G644520 for controlling cucumber non-tendril character, and a cucumber tendril character-related SNP marker and an application of the gene. The SNP marker is located at the loci 1012bp of a cucumber gene Csa5G644520 shown in Seq ID No.2; the cucumber of which the basic group at the loci 1012bp is T has no tendril character; and the cucumber of which the basic group at the loci 1012bp is A has tendril character. The gene Csa5G644520 for controlling cucumber non-tendril character and the cucumber tendril character-related SNP marker are found out by a rare SNP method, a map-based cloning method and the like. The genetype of a to-be-detected cucumber is detected through a method of a derived cleaved amplified polymorphic sequence (dCAPS); and whether the cucumber plant has tendril or not can be judged according to the genetype, so as to accelerate the breeding process of the non-tendril cucumber.

Description

technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, in particular to the gene Csa5G64452 controlling the tendrilless trait of cucumber and the SNP marker related to the tendril trait of cucumber. Background technique [0002] The absence of tendrils is an important plant type trait of cucumber. Almost all cucumber resources and varieties have tendrils, and a rare cucumber resource without tendrils was found in the cucumber varieties from Xishuangbanna area. This cucumber resource has no tendrils. The tendrils are the climbing organs of cucumbers, but under artificial cultivation conditions, the tendrils have lost their proper function and become useless organs through brackets and vines. However, the tendrils still exist on the plant as an organ, and tendrils climb and consume a lot of nutrients, and the tendrils will compete strongly with the female flowers at the same node for nutrients. In the field production of cucu...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82C12Q1/68C12N15/11C12N1/15C12N1/19C12N1/21A01H5/00
CPCC07K14/415C12N15/11C12N15/8261C12Q1/6895C12Q2600/13
Inventor 黄三文王深浩张忠华杨学勇邵光金齐建建
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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