A kit for measuring the concentration ratio of glycated albumin to albumin with a single system
A technology of glycosylated albumin and kits, which is applied in the field of medical testing and can solve problems such as many influencing factors, poor repeatability, and large coefficient of variation
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Embodiment 1
[0044] Prepare the 80mmol / L pH8.50 Tris-HCl buffer solution containing bromelain concentration of 30KU / L, TOOS concentration of 0.5mmol / L and TritonX-100 concentration of 0.5 as reagent I; containing FAOD concentration of 20KU / L, POD concentration of 5KU / L, 4-AAP concentration of 1.0mmol / L, trehalose concentration of 10g / L 80mmol / L pH8.50 Tris-HCl buffer solution is reagent II; DAO concentration of 200KU / L, trehalose concentration of 10g / L L of 80mmol / L pH8.50 Tris-HCl buffer solution is reagent Ⅲ. When the determination reagent is used to measure sample liquid, the instrument used is BECKMAN LX20 automatic biochemical analyzer, the reaction temperature is 37°C, the sample volume is 5 μl, the volume of reagent I is 140 μl, the volume of reagent II is 60 μl, and the volume of reagent III is 45μl, the main / minor wavelength is 560 / 800nm. After mixing reagent I and sample, react at the measuring temperature for 180 seconds; add reagent II and mix well and react for 180 seconds, a...
Embodiment 2
[0050] Preparation containing Bromelain concentration is 75KU / L, TOOS concentration is 2.75mmol / L, TritonX-100 concentration is 5.25 110mmol / L pH8.50 Tris-HCl buffer solution is reagent I; Containing FAOD concentration is 60KU / L, POD concentration is 17.5KU / L, 4-AAP concentration of 2.0mmol / L, trehalose concentration of 30g / L 110mmol / L pH8.50 Tris-HCl buffer solution is reagent Ⅱ; DAO concentration of 350KU / L, trehalose concentration of 30g 110mmol / L pH8.50 Tris-HCl buffer solution in / L is the reagent Ⅲ. When this assay reagent is used to measure sample liquid, the instrument used is BECKMAN CX4 automatic biochemical analyzer, the reaction temperature is 37°C, the sample volume is 5 μl, the volume of reagent I is 140 μl, the volume of reagent II is 60 μl, and the volume of reagent III is 45μl, the main / minor wavelength is 560 / 800nm. After mixing reagent I and sample, react at the measuring temperature for 240 seconds; add reagent II and mix well, react for 240 seconds, and r...
Embodiment 3
[0053] Prepare the 200mmol / L pH8.50 Tris-HCl buffer solution containing bromelain concentration of 120KU / L, TOOS concentration of 5mmol / L and TritonX-100 concentration of 10ml / L as reagent I; containing FAOD concentration of 100KU / L, POD concentration 200mmol / L pH8.50 Tris-HCl buffer solution with a concentration of 30KU / L, a concentration of 4-AAP of 3.0mmol / L, and a concentration of trehalose of 50g / L is the reagent II; the concentration of DAO is 500KU / L, and the concentration of trehalose is 50g 200mmol / L pH8.50 Tris-HCl buffer solution per L is the reagent Ⅲ. When the assay reagent is used to measure sample liquid, the instrument used is Shimadzu UV2201 UV-visible spectrophotometer, the reaction temperature is 37°C, the sample volume is 5 μl, the volume of reagent I is 140 μl, the volume of reagent II is 60 μl, and the volume of reagent III The volume is 45μl, and the main / minor wavelengths are 560 / 800nm. After mixing reagent I and sample, react at the measuring temperat...
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