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Tissue culture and rapid propagation method for divaricate saposhnikovia root

A tissue culture and callus technology, applied in the field of plant tissue culture, can solve problems such as affecting the reproduction and development of windproof seedlings, and no windproof in vitro regeneration system has been found.

Inactive Publication Date: 2015-06-10
陈桂容
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no reports about the in vitro regeneration system of the windproof, which has seriously affected the development of the windproof seedling propagation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Treatment of explants: Select the full harvested parsnip seeds of the year, gently scrub with detergent water, rinse in tap water for 10 minutes, soak in clean water for 8 hours, and then transfer to 80mg / mL gibberellin Soak in solution for 10h, then soak in 75% ethanol solution for 10s in ultra-clean workbench, rinse with sterile water for 3 times, then disinfect with 0.1% mercury liter solution containing 0.01% Tween-20 for 5min, rinse with sterile water for 3 times Finally, blot the moisture on the surface with sterile filter paper for later use.

[0020] (2) Callus induction: use a sterile scalpel to cut 1 knife on the seeds treated in step (1) to create a wound, then inoculate on the induction medium for callus induction culture, and inoculate at 28°C The callus can be formed after 25 days of total dark culture, and the induction rate is as high as 96.8%. The induction medium is: MS+ 3mg / L 2,4-D+ 0.5mg / L 6-BA+ 0.3mg / L KT+3.5% sucrose+0.35% agar+0.08% activated...

Embodiment 2

[0026] (1) Treatment of explants: Select the full harvested parsnip seeds of the year, gently scrub with detergent water, rinse in tap water for 15 minutes, soak in clean water for 8 hours, and then transfer to 110mg / mL gibberellin Soak in solution for 12h, then soak in 75% ethanol solution for 15s in ultra-clean workbench, rinse with sterile water for 5 times, then disinfect with 0.1% mercury liter solution containing 0.01% Tween-20 for 8min, rinse with sterile water for 5 times Finally, blot the moisture on the surface with sterile filter paper for later use.

[0027] (2) Callus induction: use a sterile scalpel to cut 3 times on the seeds treated in step (1) to create wounds, inoculate them on the induction medium for callus induction culture, and inoculate them at 28°C The callus can be formed after 22 days of total dark culture, and the induction rate is as high as 89.8%. The induction medium is: MS + 5 mg / L 2,4-D + 0.6 mg / L 6-BA + 0.1 mg / L KT + 3.5% sucrose + 0.35% agar...

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PUM

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Abstract

The invention discloses a tissue culture and rapid propagation method for divaricate saposhnikovia root, i.e., an in-vitro rapid propagation system of the divaricate saposhnikovia root is established by taking divaricate saposhnikovia root seeds as an explant to adapt to large-scale and standard production needs. According to the tissue culture and rapid propagation method disclosed by the invention, the seeds are taken as the explant, the tissue culture and rapid propagation of the divaricate saposhnikovia root is realized through processes of callus induction, propagation culture, adventitious bud induction, root and shoot induction as well as test tube seedling acclimatizating and transplanting and the like, and technical support is provided for the industrial production of divaricate saposhnikovia seedlings.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapid propagation of windproof tissue culture. Background technique [0002] windproof ( Saposhnikovia divaricata ) is a medicinal plant of Umbelliferae. Its dried roots are used as medicine. The traditional way to obtain windbreaks is to dig wild plant resources. Due to blind digging, not only the wild resources are decreasing day by day, but also the ecological balance of nature is seriously damaged. In addition, the traits of cultivated varieties degenerate, yield and quality decline, and cannot meet the needs of large-scale and standardized production. [0003] At present, the research on Fangfeng mainly focuses on plant resources, chemical composition analysis, crude drug identification, pharmacological effects and clinical application, etc. These studies have laid a certain foundation for the clinical application of Fa...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈桂容
Owner 陈桂容