Stable and efficient actinobacillus protein extraction method suitable for dimensional electrophoresis

Abstract

The invention relates to a stable and efficient actinobacillus protein extraction method suitable for dimensional electrophoresis. The method provided by the invention is obtained by optimizing Actinobacillus pleuropneumoniae protein extraction methods, combines liquid nitrogen grinding with ultrasonic concussion, and combines a phenol solution with an ammonium acetate solution, so the protein extraction efficiency is improved. The method has the characteristics of simple operation, labor and time saving, high protein extraction efficiency and few interferents. The method is suitable for materials with the disadvantages of difficult protein extraction, low protein extraction yield and many interference impurities. Proteins obtained in the invention have the characteristics of easy re-dissolving, few impurities and interferents, and complete meeting of first direction and second direction requirements of dimensional electrophoresis, allow an atlas with the characteristics of high resolution, good repeatability, uniform distribution of protein points and intelligible protein points to be obtained, and are of great significance for researching the epigenetics of Actinobacillus pleuropneumoniae, screening of immune proteins, and screening of vaccine candidate proteins.

Description

technical field [0001] The invention relates to a protein extraction method, in particular to a stable and efficient actinomycete protein extraction method suitable for two-dimensional electrophoresis, and belongs to the field of biotechnology. Background technique [0002] Porcine contagious pleuropneumonia, also known as necrotizing pleuropneumonia, is an acute respiratory infectious disease caused by Actinobacillus pleuropneumoniae, mainly acute hemorrhagic fibrinous pneumonia and chronic fibrinous necrotizing pleurisy feature. The case fatality rate of the acute patient is high, and the chronic patient can often endure it. The disease was first reported by Pattison in England in 1957. At present, the disease is widespread in the world and has caused huge economic losses. The United States, Denmark, and Switzerland list this disease as one of the main pig diseases. Due to the frequent introduction of species in our country in recent years, the disease has also invaded...

AI Technical Summary

Problems solved by technology

There are many protein extraction methods, but none of them are universal, so different measures should be taken for different biological tissues

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