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Application of schisandra chinensis in inhibition of ras proto-oncogene overexpression

A proto-oncogene, Schisandra chinensis technology, which is applied to plant raw materials, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., can solve the problem of no literature report on Schisandra chinensis, etc., and achieves widening the scope of medication and better treatment. Effect

Inactive Publication Date: 2015-06-17
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no literature report that Schisandra chinensis can inhibit the overexpression of ras proto-oncogene, and there is no drug using Schisandra chinensis as a raw material on the market, which can specifically inhibit the tumor caused by the overexpression of ras proto-oncogene

Method used

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  • Application of schisandra chinensis in inhibition of ras proto-oncogene overexpression
  • Application of schisandra chinensis in inhibition of ras proto-oncogene overexpression
  • Application of schisandra chinensis in inhibition of ras proto-oncogene overexpression

Examples

Experimental program
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Effect test

Embodiment 6

[0011] The preparation of embodiment one schisandra medicinal liquid

[0012] Schisandra source: purchased from Lanzhou Yellow River Medicine Market.

[0013] Weigh 9 g of Schisandra chinensis; first soak in distilled water for 20 minutes, then boil at a slow fire, decoct for 30 minutes, constant volume, centrifuge, discard the precipitate, filter the supernatant aseptically, put it in a 4°C refrigerator for later use, and dilute to different concentrations when used.

[0014] Example 1 The effect of Schisandra medicinal liquid on Caenorhabditis elegans MT2124 (let-60 hyperactivation mutation)

[0015] Ras is a small molecular protein that participates in the regulation of many important links in animal development. The ras signaling pathway is highly conserved from nematodes to humans. The let-60 gene encodes a conserved Ras protein in Caenorhabditis elegans, which has 83% homology with human Ras proteins. If the ras mutation, in Caenorhabditis elegans, it will cause th...

Embodiment 8

[0034] Example 3 The effect of Schisandra chinensis on Caenorhabditis elegans MT8189 (lin-15 mutation inactivation)

[0035] Experimental materials: Caenorhabditis elegans MT8189, polyvulva phenotype, genotype: lin-15b(n765)X, purchased from CGC (Caenorhabditis Genetics Center); Escherichia coli OP50 (uracil leaky mutant strain), used as Caenorhabditis elegans Nematode food was purchased from CGC (Caenorhabditis Genetics Center).

[0036] Concrete experimental procedure: with embodiment two.

[0037] Table 2 Effect of Schisandra chinensis on Caenorhabditis elegans MT8189 (lin-15 mutation inactivation)

[0038]

[0039] In Caenorhabditis elegans, lin-15(n765)X is located upstream of ras and normally inhibits the expression of ras gene. The inactivation of this gene in the MT8189 nematode strain leads to the phenotype of multiple ovipositors in the nematode.

[0040] As shown in the table, Schisandra can not inhibit the polyvulva phenotype of lin-15(n765)X. According to...

Embodiment 9

[0041] Example 4 Effect of Schisandra chinensis on Caenorhabditis elegans CB1275 (lin-1 mutation inactivation)

[0042] Experimental materials: Caenorhabditis elegans genotype: lin-1(e1275) IV was obtained from CGC (Caenorhabditis Genetics Center); Escherichia coli OP50 (uridine leaking mutant strain), as the food of Caenorhabditis elegans, was purchased from CGC.

[0043] Concrete experimental procedure: with embodiment two.

[0044] Table 3 The effect of Schisandra chinensis on Caenorhabditis elegans CB1275 (lin-1 mutation inactivation)

[0045]

[0046] lin-1 is a gene downstream of ras, a nuclear transcription factor that can be phosphorylated by MAPK, a negative regulator of vulva development, and a signaling pathway that regulates vulva development downstream of let-60. After lin-1 mutation inactivation, nematodes have polyvaginal phenotype. Since lin-1 is a nuclear transcription factor, it regulates the process of vulva cell development. If the drug has a signif...

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Abstract

The invention relates to an application of schisandra chinensis in inhibition of ras proto-oncogene overexpression, and specifically, relates to the application of the schisandra chinensis in preparation of anti-tumor drugs for inhibiting ras proto-oncogene overexpression. Researches indicate that about 90% of pancreatic cancer, 50% of colon cancer, 30%-40% of lung adenocarcinoma and 5%-40% of leukemia are caused by the ras proto-oncogene overexpression, and a Ras protein becomes a universally accepted target for screening drugs resistant to related malignant tumors. Animal experiments indicate that schisandra chinensis can specifically down-regulate overexpression caused after ras proto-oncogene mutation, but does not act on wild-type ras proto-oncogenes, so that the toxicity is low. The invention provides the new use of schisandra chinensis, and schisandra chinensis can be applied in preparation of the anti-tumor (tumors caused by the ras proto-oncogene overexpression) drugs.

Description

technical field [0001] The invention specifically relates to the application of schisandra chinensis in inhibiting the overexpression of ras proto-oncogene, and belongs to the field of traditional Chinese medicine. Background technique [0002] Schisandra Schisandra chinensis It is a deciduous woody vine, warm in nature, sweet in taste, slightly sour, has the effects of astringent and astringent, nourishing qi and promoting body fluid, nourishing kidney and calming heart, etc. It is one of the commonly used traditional Chinese medicines. Schisandra has a wide range of pharmacological effects, such as cardiac strengthening, anti-liver damage, sedation and stability, anti-tumor, immune regulation and other effects. [0003] About 30% of human tumors are caused by the overactivation of Ras proto-oncogene after mutation, resulting in increased expression of Ras protein. If the Ras protein is continuously activated, it can bind to the downstream effector protein and transmit th...

Claims

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Application Information

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IPC IPC(8): A61K36/57A61K36/79A61P35/00
Inventor 李红玉李孟惠万凤奇支德娟李洋黄坚张占欣陈晓雨李莹辉
Owner LANZHOU UNIVERSITY
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