Application of dendrobium nobile alkaloid
A technology of Dendrobium dendrobii and alkaloids, which is applied to medical preparations, drug combinations, plant raw materials and other directions containing active ingredients, can solve problems such as peripheral nerve damage that have not yet been found, and achieve the effect of expanding the scope of drugs
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Embodiment 1
[0016] Example 1: To observe the effect of dendrobine on sciatic nerve function in sciatic nerve anastomosis model rats.
[0017] 1.1. Establishment of a rat sciatic nerve anastomosis model
[0018] The rats were randomly divided into 5 groups (8 rats in each group): blank group, model group, low-dose dendrobine group, high-dose dendrobine group and methylcobalamin group. 200 ± 20 g male SD rats were anesthetized by intraperitoneal injection of 3% sodium pentobarbital, fixed in a supine position, the inner skin of the left hind limb of the rats was sterilized with iodophor, a skin incision of about 3 cm was made, and the space of the vastus medialis muscle was bluntly separated. , expose the sciatic nerve, cut the nerve and apply 9-0 microstrip suture to perform tension-free epineurium end-to-end anastomosis on the sciatic nerve, and establish a sciatic nerve anastomosis model, which is suitable for studying muscle atrophy and functional recovery after denervation injury .
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Embodiment 2
[0027] Example 2: To observe the effect of dendrobine on the regeneration of injured nerves in the sciatic nerve anastomosis model rats.
[0028] 2.1. Observation of pathological changes of motor neurons in the anterior horn of the spinal cord
[0029] Take the rat L 4-6 The spinal cord was stripped and embedded in paraffin after paraffin fixation. Nissl staining and HE staining were performed on the paraffin section of the spinal cord to observe the pathological changes of motor neurons in the anterior horn of the spinal cord. The specific steps of Nissl staining (Solarbio, Cat#G1436) are as follows: (1) Paraffin sections were 3 μm thick and routinely dewaxed to water. (2) Dip with Nissl staining solution (toluidine blue method) in a 50-60 ℃ incubator for 20-40 min. Wash with distilled water. (3) Rapid differentiation in 95% ethanol. (4) Dehydrate in absolute ethanol, clear in xylene, and mount with neutral gum. The HE staining procedure was as previously described.
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