Application of dendrobium nobile alkaloid

A technology of Dendrobium dendrobii and alkaloids, which is applied to medical preparations, drug combinations, plant raw materials and other directions containing active ingredients, can solve problems such as peripheral nerve damage that have not yet been found, and achieve the effect of expanding the scope of drugs

Pending Publication Date: 2022-07-22
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dendrobine ( Dendrobine ) is the main representative component in DNLA. In the 2015 edition of the "Chinese Pharmacopoeia", the content of dendrobine is used as the quality inspection index of Dendrobium nobile, and its content is stipulated not to be less than 0.4%. It is speculated that it may be the active ingredient for DNLA to exert neuroprotective effects One, at present, the protective effect of dendrobine on the central nervous system needs to be further elucidated, and no report on its application to peripheral nerve injury has been found yet.

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  • Application of dendrobium nobile alkaloid
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  • Application of dendrobium nobile alkaloid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: To observe the effect of dendrobine on sciatic nerve function in sciatic nerve anastomosis model rats.

[0017] 1.1. Establishment of a rat sciatic nerve anastomosis model

[0018] The rats were randomly divided into 5 groups (8 rats in each group): blank group, model group, low-dose dendrobine group, high-dose dendrobine group and methylcobalamin group. 200 ± 20 g male SD rats were anesthetized by intraperitoneal injection of 3% sodium pentobarbital, fixed in a supine position, the inner skin of the left hind limb of the rats was sterilized with iodophor, a skin incision of about 3 cm was made, and the space of the vastus medialis muscle was bluntly separated. , expose the sciatic nerve, cut the nerve and apply 9-0 microstrip suture to perform tension-free epineurium end-to-end anastomosis on the sciatic nerve, and establish a sciatic nerve anastomosis model, which is suitable for studying muscle atrophy and functional recovery after denervation injury .

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Embodiment 2

[0027] Example 2: To observe the effect of dendrobine on the regeneration of injured nerves in the sciatic nerve anastomosis model rats.

[0028] 2.1. Observation of pathological changes of motor neurons in the anterior horn of the spinal cord

[0029] Take the rat L 4-6 The spinal cord was stripped and embedded in paraffin after paraffin fixation. Nissl staining and HE staining were performed on the paraffin section of the spinal cord to observe the pathological changes of motor neurons in the anterior horn of the spinal cord. The specific steps of Nissl staining (Solarbio, Cat#G1436) are as follows: (1) Paraffin sections were 3 μm thick and routinely dewaxed to water. (2) Dip with Nissl staining solution (toluidine blue method) in a 50-60 ℃ incubator for 20-40 min. Wash with distilled water. (3) Rapid differentiation in 95% ethanol. (4) Dehydrate in absolute ethanol, clear in xylene, and mount with neutral gum. The HE staining procedure was as previously described.

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Abstract

The invention relates to application of dendrobium nobile alkaloid. The application refers to application of the dendrobium nobile alkaloid to preparation of a medicine for treating peripheral nerve injury. According to the invention, it is clear that dendrobine can accelerate nerve regeneration and functional recovery after peripheral nerve injury, and the effective dose of dendrobine is 40 mg / kg; according to the application, the medication range of the dendrobium nobile is expanded, and a theoretical basis is provided for applying the dendrobine to clinical treatment of peripheral nerve injury.

Description

technical field [0001] The invention relates to new uses of traditional Chinese medicine products, in particular to the use of alkaloids of Dendrobium chinensis. Background technique [0002] peripheral nerve injury (Peripheral nerve injury, PNI) It is one of the common clinical traumas. Due to the slow regeneration of nerves after injury, the sensory and functional recovery of nerve target organs is incomplete, which reduces the quality of life of patients and increases the burden on families. Therefore, finding strategies or means that can accelerate nerve regeneration is of great significance for the clinical treatment of peripheral nerve injury. [0003] Dendrobium Dendrobium ( Dendrobium nobile ) is a perennial herb of Orchidaceae, which is a traditional and precious traditional Chinese medicine in my country. It mainly grows in areas with higher altitudes such as Yunnan, Guizhou and Sichuan. Its main chemical components are alkaloids, polysaccharides, flavonoids, ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/407A61K36/8984A61P25/02
CPCA61K31/407A61K36/8984A61P25/02
Inventor 聂晶孙广峰涂星星
Owner ZUNYI MEDICAL UNIVERSITY
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