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Method for producing epsilon-polylysine through fermentation of cassava starch

A technology of tapioca starch and polylysine is applied in the field of fermentation engineering to achieve the effects of reducing cost, reducing pollution and low price

Inactive Publication Date: 2015-06-24
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Starch comprises cornstarch, potato starch, modified starch etc., and wherein cassava starch is called " king of potato starch ", is fermented with cassava starch, and it can be seen that Wang Yanfei et al. Alcohol and Zhu Du et al. (CN 101974573 A) are used to ferment and produce microbial oils, etc., but there is no report on the method of using it as raw material to ferment and produce ε-PL

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Ingredients: The formula of the fermentation medium is: cassava starch 60g / L, fish meal 15g / L, (NH 4 ) 2 SO 4 10g / L, MgSO 4 ·7H 2 O 0.5g / L, K 2 HPO 4 0.8g / L, KH 2 PO 4 1.36g / L, FeSO 4 ·7H 2 O 0.03g / L, ZnSO 4 ·7H 2 O 0.04g / L, adjust the pH to 6.8.

[0019] (2) Sterilization: maintain the prepared medium at 118°C for 20 minutes, and then lower it to 30°C.

[0020] (3) Fermentation: Inoculate the 24-h seed liquid with a 10% inoculation amount into the above-mentioned fermentation medium using tapioca starch as a carbon source. Cultivate for 72 hours under the condition of shaking table with temperature of 30°C and rotation speed of 200r / min, collect the fermentation liquid, and measure the yield of ε-PL as 0.954g / L.

Embodiment 2

[0022] (1) Ingredients: The formula of the fermentation medium is: cassava starch 60g / L, fish meal 15g / L, (NH 4 ) 2 SO 4 5g / L, MgSO 4 ·7H 2 O 0.5g / L, K 2 HPO 4 0.8g / L, KH 2 PO 4 1.36g / L, FeSO 4 ·7H 2 O 0.03g / L, ZnSO 4 ·7H 2 O 0.04g / L, adjust the pH to 6.8.

[0023] (2) Sterilization: maintain the prepared medium at 118°C for 30 minutes, and then lower it to 30°C.

[0024] (3) Fermentation: Inoculate the seed liquid obtained through shaking culture for 44 hours into the above-mentioned fermentation medium using tapioca starch as a carbon source with an inoculum size of 10%. Fermentation was carried out in a fermenter with an initial pH of 6.8 and a temperature of 30°C. When the initial pH of the fermentation dropped to 3.8, the pH was maintained. Stirring culture was carried out for 192 hours, and the fermentation liquid was collected. The yield of ε-PL was measured to be 27.556g / L .

Embodiment 3

[0026] (1) Ingredients: The formula of the fermentation medium is: tapioca starch 20g / L, fish meal 5g / L, (NH 4 ) 2 SO 4 4g / L, MgSO 4 ·7H 2 O 0.5g / L, K 2 HPO 4 0.8g / L, KH 2 PO 4 1.36g / L, FeSO 4 ·7H 2 O 0.03g / L, ZnSO 4 ·7H 2 O 0.04g / L, adjust the pH to 6.5.

[0027] (2) Sterilization: maintain the prepared medium at 115°C for 15 minutes, and then lower it to 30°C.

[0028](3) Fermentation: Inoculate the 24-h seed liquid with a 10% inoculation amount into the above-mentioned fermentation medium using tapioca starch as a carbon source. Cultivate for 72 hours under the condition of shaking table with temperature of 30°C and rotation speed of 200r / min, collect the fermentation liquid, and measure the yield of ε-PL to be 0.408g / L.

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Abstract

The invention relates to a method for producing epsilon-polylysine through the fermentation of cassava starch, which belongs to the field of fermentation engineering. The method disclosed by the invention is implemented by taking epsilon-polylysine producing bacteria as a fermentative strain, taking cassava starch as a carbon source, and fermenting under certain conditions so as to produce epsilon-polylysine. According to the invention, epsilon-polylysine is produced through fermenting by taking cassava starch as a raw material, so that the selection of the carbon source for epsilon-polylysine fermentation is expanded; and cassava resources are fully used, so that the raw material cost for the fermentation production of epsilon-polylysine is reduced, and therefore, the method has huge economic, social and ecological benefits. The method disclosed by the invention lays a solid technical foundation for the application of cassava starch in the fermentation production of epsilon-polylysine, and has a good industrial application prospect.

Description

technical field [0001] The invention relates to a method for fermenting and producing ε-polylysine with tapioca starch, in particular to a new method for fermenting and producing ε-polylysine with cassava starch instead of glucose as a single carbon source, and belongs to the field of fermentation engineering. Background technique [0002] ε-polylysine (ε-PL) is a secondary metabolic linear polymer obtained by microorganisms through aerobic fermentation. It consists of only 25-35 L-lysine residues through α-carboxyl and ε- Amino linked. It can be used as a food preservative, and has the advantages of broad antibacterial spectrum, high safety performance, good thermal stability, and convenient use, which makes it the most commercially potential biological preservative. ε-PL was approved by the FDA (food and drug administration) as a safe food preservative in October 2003, and has been widely used in the United States, Japan and South Korea, and my country’s Health and Family...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02
Inventor 扶教龙吴晨奇苏曲辰钱大伟胡翠英陈宏伟邱业先
Owner SUZHOU UNIV OF SCI & TECH
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