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Detection method of triglyceride content in microalgae

A technology of triglyceride and detection method is applied in the field of detection of triglyceride content in microalgae, and achieves the effects of low power consumption, resource saving and short wall breaking time

Active Publication Date: 2016-10-26
JIANGSU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Generally speaking, the selected organic solvent should strive to obtain a high oil yield during the extraction process while avoiding the damage of the solvent to the human body. Studies have shown that it is difficult to extract the intracellular oil of microalgae with a single solvent

Method used

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  • Detection method of triglyceride content in microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (Example 1, the extraction method of microalgae oil)

[0046] The extraction method of the microalgae oil of the present embodiment comprises the following steps:

[0047] 1. Get fresh microalgae grown to the end of the logarithm (the microalgae in this embodiment is the diatom Chaetoceros, Chaetoceros gracilis , provided by the University of Minnesota, USA) 1000mL of culture medium was centrifuged at 8000r / min for 15 minutes, the supernatant was removed and the obtained microalgae were freeze-dried, and the weight change of the microalgae was measured every 12h during the freeze-drying process. After constant weight ( In this example, after 72 hours of freeze-drying, the microalgae powder has a constant weight) Weigh 0.2 g of the algae powder into a 15 mL test tube.

[0048] ② Prepare 100 mL of a mixed solution with tetrahydrofuran: chloroform: n-hexane at a volume ratio of 1:1:1 for use.

[0049] ③Use a pipette to pipette 5mL of the mixed solution prepared in step ...

Embodiment 2

[0056] (Example 2, the extraction method of microalgae oil)

[0057] The extraction method of the microalga oil of the present embodiment all the other are identical with embodiment 1, difference is:

[0058] When preparing the mixed solution in step ②, the volume ratio of tetrahydrofuran: chloroform: n-hexane is 1:1.5:1. The quality of the oil that step 5. extracts is 0.076g.

Embodiment 3

[0059] (Example 3, the extraction method of microalgae oil)

[0060] The extraction method of the microalga oil of the present embodiment all the other are identical with embodiment 1, difference is:

[0061] When preparing the mixed solution in step ②, the volume ratio of tetrahydrofuran: chloroform: n-hexane is 1:0.8:1. The quality of the oil that step 5. extracts is 0.079g.

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Abstract

The invention discloses a detection method for the content of triglyceride in microalgae. When microalgae grease is extracted, a mixed solvent is adopted; the mixed solvent and microalgae powder are mixed and then are subjected to ultrasonic wall-cracking extraction; and the wall cracking rate of the microalgae is up to 99%, the microalgae time is very short, and the efficiency for extracting the triglyceride in microalgae cells in a whole process is high. The detection method for the content of the triglyceride in the microalgae adopts a gas-phase method; and the gas-phase method can be used for effectively and accurately detecting the content of the triglyceride.

Description

[0001] This application is a divisional application of the invention patent application with the application number 201310585841.9 and the application date on November 20, 2013, and the invention name is "the extraction method of microalgae oil and the detection method of triglyceride content in microalgae". technical field [0002] The invention relates to a method for extracting oil in microalgae cells and a method for detecting the content of triglyceride contained in microalgae cells. Background technique [0003] The world's crude oil production continues to decrease and is expected to drop to 7.5 billion barrels per year by 2018, which is only equivalent to the crude oil production in 1950. With the increasing depletion of petroleum resources, global energy shortage and environmental pollution, the development of green and clean biofuels to replace traditional fossil fuels has become an international research hotspot. [0004] Biodiesel refers to organic fatty acid est...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 梁国斌刘维平朱政滔
Owner JIANGSU UNIV OF TECH