A strain of low-temperature aerobic denitrifying bacteria and its application
An aerobic denitrifying bacteria, low temperature technology, applied in the field of environmental microorganisms and environmental engineering, can solve the problems of difficulty in denitrification of nitrate sewage, and achieve the effects of degrading COD, reducing costs, and effectively denitrifying COD
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] Obtaining low-temperature aerobic denitrifying bacteria:
[0027] A. Collect samples from the waters of Buha River in Qinghai, isolate and identify single colonies.
[0028] B. Cultivate the strain isolated in step A in a beef extract peptone liquid medium at 15°C to obtain a low-temperature bacteria that can grow at 15°C.
[0029] C. Inoculate the low-temperature bacteria selected in step B into a denitrification medium, culture at 15°C, and determine and screen high-efficiency low-temperature denitrifying bacteria by measuring the content of nitrate, nitrite, and total nitrogen in the medium.
[0030] Finally, a low-temperature denitrifying bacterium was obtained. The bacterium was sent to the China Type Culture Collection for preservation on March 9, 2015, under the classification name: Pseudomonas brassicacearum subsp.brassicacearum BW0806, and the preservation number is: CCTCC NO:M 2015094, Address: Wuhan University, Wuhan, China.
[0031] The low-temperature aerobic denitr...
Embodiment 2
[0034] Suitable temperature for growth of Pseudomonas brassicacearum subsp. Brassicacearum BW0806:
[0035] Cultivate the low-temperature aerobic denitrifying bacteria to the logarithmic growth phase and inoculate it in the denitrification medium to adjust the initial OD 600 About 0.05, culture in a sealed shake flask at 120 rpm at different temperatures, and determine the nitrate, nitrite, total nitrogen content and OD600 value of the culture medium every 24 hours (Table 1). The parameter values measured above (except OD 600 ) Are the relevant values in the supernatant after centrifugation of the cells.
[0036] Table 1
[0037]
[0038] The results show that the low-temperature aerobic denitrifying bacteria are adapted to growth and denitrification reactions at 15°C. The degradation rate of nitrate in eight days is 100%, and the degradation rate of total nitrogen is about 97%. In the denitrification medium at a culture temperature of 10°C, the degradation rate of nitrate on th...
Embodiment 3
[0040] Suitable C / N ratio for growth of Pseudomonas brassicacearum subsp.brassicacearum BW0806:
[0041] At 15℃, cultivate the low-temperature aerobic denitrifying bacteria to the logarithmic growth phase and respectively inoculate them in denitrification media with different carbon to nitrogen ratios (the carbon to nitrogen ratio is adjusted by adding different quality sodium acetate) to adjust the initial OD600 to about 0.05 , 120rpm sealed shake flask culture, every 24h to determine the nitrate, nitrite, total nitrogen content and OD600 value in the medium. The parameter values measured above (except OD 600 ) Are the relevant values in the supernatant after centrifugation of the cells.
[0042] Table 2
[0043]
[0044] The test results show that the low-temperature aerobic denitrifying bacteria are adapted to grow and denitrify in a medium with a low carbon to nitrogen ratio (2-10). When C / N=5, the removal rate of nitrate on the third day is 100%, and the removal rate of to...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More