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Compound enzymolysis method for quickly preparing galactomannan oligosaccharide

A compound enzymatic hydrolysis and mannanase technology, which is applied in the field of compound enzymatic hydrolysis for the rapid preparation of galactomannose, can solve the problems of long time, low efficiency, low oligosaccharides, etc., and save production time and cost , good enzymatic hydrolysis method, no toxic and side effects

Active Publication Date: 2015-07-01
临沂小鲁生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is that the existing method mainly adopts β-mannanase to the enzymatic hydrolysis of guar gum, but the efficiency of adopting a single enzyme to hydrolyze guar gum is not high
Although some existing technologies have adopted the method of degrading plant polysaccharides with compound enzymes, since each enzyme degrades separately, the time is long and the efficiency is low
The 2nd, in the guar gum enzymatic hydrolysis process, because the guar gum solution viscosity is too high, the concentration during the enzymolysis guar gum that generally adopts at present is 0.3-2%, the concentration of the oligosaccharide that obtains is lower, in further When preparing galactomannose powder, dehydration and drying treatment is required, which not only increases the cost of subsequent dehydration and drying of the product, galactomannose, but also seriously affects the efficiency of industrial production

Method used

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  • Compound enzymolysis method for quickly preparing galactomannan oligosaccharide
  • Compound enzymolysis method for quickly preparing galactomannan oligosaccharide
  • Compound enzymolysis method for quickly preparing galactomannan oligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Mix 8 g of guar gum powder with 400 U of β-mannanase, 8000 U of xylanase, and 8000 U of cellobiase; then add 0.2 mol / mL citric acid-sodium citrate buffer solution to 100 mL, adjust the pH 6.0; the swelling temperature is 26°C, and the swelling is 30 minutes; then the solution is heated to 50°C, and enzymatically hydrolyzed for 6 hours; The mass percentages of galactomannose oligosaccharides with different degrees of polymerization in the enzymatic hydrolysis solution of this example measured by MALDI-TOF and HPLC methods are shown in Table 1. 95.3% of the composition.

[0028] The mass percent of different degree of polymerization galactomannose in the embodiment 1 of table 1

[0029]

Embodiment 2

[0031] Mix 10g of guar gum powder with 600U of β-mannanase, 20000U of xylanase and 15000U of cellobiase; then add 0.2mol / mL citric acid-sodium citrate buffer solution to 100mL, adjust the pH 6.3; the swelling temperature is 33°C, swelling for 60 minutes; then the solution is heated to 52.5°C, and enzymatically hydrolyzed for 8 hours; after the enzymatic hydrolysis, the enzymatic hydrolyzate of galactomannose oligosaccharides is obtained. The mass percentages of galactomannose oligosaccharides with different degrees of polymerization in the enzymatic hydrolyzate of this example measured by MALDI-TOF and HPLC methods are shown in Table 2, and galactomannose oligosaccharides with a degree of polymerization of 2-10 account for the sugars in the enzymatic hydrolyzate 97.1% of ingredients.

[0032] The mass percent of different degree of polymerization galactomannose in the embodiment 2 of table 2

[0033]

Embodiment 3

[0035] Mix 12g of guar gum powder with 840U of β-mannanase, 36000U of xylanase and 24000U of cellobiase; then add 0.2mol / mL citric acid-sodium citrate buffer solution to 100mL, adjust the pH 6.6; the swelling temperature is 38°C, swelling for 90 minutes; then the solution is heated to 55°C, and enzymatically hydrolyzed for 10 hours; after the enzymatic hydrolysis, the enzymatic hydrolyzate of galactomannose oligosaccharides is obtained. The mass percentages of galactomannose oligosaccharides with different degrees of polymerization in the enzymatic hydrolysis solution of this example measured by MALDI-TOF and HPLC methods are shown in Table 3, and galactomannose oligosaccharides with a degree of polymerization of 2-10 account for the sugars in the enzymatic hydrolysis solution 98.1% of ingredients.

[0036] The mass percent of different degree of polymerization galactomannose in the embodiment 3 of table 3

[0037]

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Abstract

The invention discloses a compound enzymolysis method for quickly preparing galactomannan oligosaccharide. The method is characterized by comprising the following steps: firstly, fully mixing guar gum powder with beta-mannase, xylanase and cellobiase; then adding a citric acid-sodium citrate buffer solution to prepare a mixed solution of guar gum and three enzymes; and after the guar gum is swelled, controlling the hydrolysis temperature and time for degradation. The method disclosed by the invention realizes hydrolysis of the three enzymes at the same time, so that the purification step of a single enzyme after sequence degradation is canceled, the production time is shortened and the cost is lowered, and the efficiency is improved. Enzymolysis of the guar gum is realized under a high concentration, and a galactomannan oligosaccharide enzymatic hydrolysate is obtained, so that the dehydrating and drying costs for subsequently producing galactomannan oligosaccharide are lowered.

Description

technical field [0001] The invention relates to a compound enzymatic hydrolysis method for rapidly preparing galactomannose, belonging to the field of biotechnology. Background technique [0002] Guar gum is a macromolecular natural hydrophilic colloid processed from the endosperm of guar beans. The main component is galactomannan, which is a natural thickener and quality improver. The appearance is white or yellowish free-flowing powder, which can be dissolved in cold water or hot water, and forms a gel-like substance when it meets water, achieving the effect of rapid thickening. The linear backbone of galactomannans consists of mannose sugars linked together by β-(1-4) glycosidic bonds, with α-(1-6) glycosidic bonds forming branch points linking galactose units. Galactomannose is an incomplete degradation product of galactomannan. It is a general term for oligosaccharides polymerized by 2-10 galactose and mannose molecules through glycosidic bonds. The molar ratio of gala...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 刘云国徐云峰刘艳华马云刘凌霄
Owner 临沂小鲁生物科技有限公司
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