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Microporous membrane osmotic emulsification method for preparing gelatin microspheres immobilized alcohol dehydrogenase

The technology of alcohol dehydrogenase and membrane emulsification method is applied in the field of microporous membrane osmotic emulsification to prepare gelatin microspheres, which can solve the problems of wide particle size distribution of microspheres, achieve simple and easy preparation process, excellent acid-base tolerance, Fixed ability strong effect

Inactive Publication Date: 2018-09-21
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microspheres obtained by this method have a wide particle size distribution and are not used to immobilize alcohol dehydrogenase

Method used

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  • Microporous membrane osmotic emulsification method for preparing gelatin microspheres immobilized alcohol dehydrogenase
  • Microporous membrane osmotic emulsification method for preparing gelatin microspheres immobilized alcohol dehydrogenase
  • Microporous membrane osmotic emulsification method for preparing gelatin microspheres immobilized alcohol dehydrogenase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of gelatin microspheres immobilized with alcohol dehydrogenase by membrane emulsification method:

[0027] Under the condition of magnetic stirring at 200 rpm and temperature at 55°C, add 1 gram of dry gelatin powder to 10 ml of water, and maintain magnetic stirring at this speed for 0.5 hours until the dry powder of gelatin is completely dissolved in water, and the concentration of gelatin after dissolution is 0.1 g / Milliliter, add 1 milligram of alcohol dehydrogenase to this gelatin solution, the concentration of alcohol dehydrogenase after dissolving is 0.1 mg / ml. The above gelatin / alcohol dehydrogenase aqueous solution was used as the aqueous phase.

[0028] The oil-soluble emulsifier Span-80 is added to 60 ml of liquid paraffin, and the quality of Span-80 is 1.5% of that of the liquid paraffin. Stir magnetically at a speed of 200 rpm for 30 minutes until Span-80 is completely dissolved, and heat the above solution to 60° C. as the oil phase.

[0029]...

Embodiment 2

[0037] The preparation process of this example is the same as that of Example 1, except that the microporous membrane with a membrane pore size of 3.5 microns is replaced by a microporous membrane with a membrane pore size of 5.0 microns to obtain a gelatin microporous membrane immobilized with alcohol dehydrogenase. The particle size and distribution of the balls are shown in Table 1.

Embodiment 3

[0039] The preparation process of this example is the same as that of Example 1, except that the microporous membrane with a membrane pore size of 3.5 microns is replaced by a microporous membrane with a membrane pore size of 7.2 microns to obtain a gelatin microporous membrane immobilized with alcohol dehydrogenase. The particle size and distribution of the balls are shown in Table 1.

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Abstract

The invention relates to a preparing method for a gelatin microsphere of fixed alcohol dehydrogenase by micro-porous membrane permeation and emulsification. The method comprises the following steps that solid gelatin and alcohol dehydrogenase are dissolved in deionized water, and a gelatin / alcohol dehydrogenase solution called as a water phase is prepared; oil soluble emulsifier span-80 is added into liquid paraffin, and liquid paraffin oil called as an oil phase is prepared; a micro-porous membrane is immersed into the liquid paraffin oil, according to the volume ratio of the gelatin / alcohol dehydrogenase solution and the liquid paraffin oil, the gelatin / alcohol dehydrogenase solution is pressed to penetrate through the micro-porous membrane and enter into the liquid paraffin oil, and W-shaped or O-shaped emulsion is formed by the emulsification; glutaraldehyde is added into the W-shaped or O-shaped emulsion, and after cross linking, solidifying, washing and drying are carried out, the gelatin microsphere of the fixed alcohol dehydrogenase is obtained. The preparing method for the gelatin microsphere of the fixed alcohol dehydrogenase by the micro-porous membrane permeation and emulsification has the advantages that the preparing condition is warm, the preparing technology is simple and easy to carry out, the granularity of the prepared microsphere is even, the size is controllable, the storing stability is good, the leakage rate of the alcohol dehydrogenase in the using process is low, and the anti-acid-base environment capacity is strong.

Description

technical field [0001] The invention relates to a method for preparing gelatin microspheres through permeation and emulsification of microporous membranes, and belongs to the technical field of gelatin microspheres with immobilized enzymes. Background technique [0002] Gelatin microspheres are a commonly used carrier for immobilized enzymes. Gelatin is a product of partial hydrolysis of animal collagen, a linear polymer formed by cross-linking 18 amino acids and polypeptides. Gelatin is non-toxic, almost non-antigenic, has good biodegradability and biocompatibility, and is cheap. It is a natural polymer material that is very suitable for preparing microspheres. Gelatin microspheres are also widely used in the preparation of embolic agents, drug carriers, tissue repair, etc., and can also be used as templates to prepare various materials with advanced structures. [0003] Existing technical methods for preparing gelatin microspheres immobilizing alcohol dehydrogenase inclu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/04
Inventor 李健姜涛马俊王艳环邵怀启陈延辉曹晨刚
Owner TIANJIN UNIV OF SCI & TECH
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