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Small-caliber biotic artificial blood vessel with anti-thrombus formation and anti-intimal hyperplasia functions

A bioartificial, intimal hyperplasia technology, applied in the field of bioartificial blood vessels, to achieve the effect of effectively removing immunogenicity and good biocompatibility

Inactive Publication Date: 2015-07-15
广州宏畅生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the huge demands and difficulties faced by small-diameter bioartificial blood vessels, the present invention uses neurotrophic factors to modify bioartificial blood vessels for the first time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. In vivo construction of a bioartificial blood vessel for anti-thrombosis and intimal hyperplasia, the vascular matrix material is prepared according to the above step 1, the vascular matrix material is modified in step 2, the functional molecule NGF coupling is carried out according to step 3, and the bioartificial blood vessel is carried out according to step 4 In vivo construction.

[0021] After the bioartificial blood vessel was transplanted to the rat carotid artery, the NGF-modified bioartificial blood vessel prepared by the present invention remained unobstructed after one month, and the average blood flow was 6.5ml / min, which was significantly higher than that of the control group. HE staining of frozen sections showed no thrombus formation and intimal hyperplasia in blood vessels, but thrombus formation and blockage in the control group. Immunofluorescent staining showed contiguous endothelial cells. Observation under the laser confocal microscope ...

Embodiment 2

[0022] Example 2. A high-patency small-caliber bioartificial blood vessel is constructed in vivo, the vascular matrix material is prepared according to the above step 1, the vascular matrix material is modified in step 2, the functional molecule brain-derived neurotrophic factor BDNF is coupled according to step 3, and the step 4 is carried out In vivo construction of bioartificial blood vessels.

[0023] After the bioartificial blood vessel was transplanted into the rat carotid artery, the BDNF-modified bioartificial blood vessel prepared by the present invention remained unobstructed after 2 months, and the patency rate was 90%, while that of the control group was only 10%. HE staining of frozen sections showed no thrombus formation and intimal hyperplasia in blood vessels, but thrombus formation and blockage in the control group. Immunofluorescent staining showed continuous endothelial cells and normal reconstitution of smooth muscle cells. Observation under the laser co...

Embodiment 3

[0024] Example 3. A small-caliber biological artificial blood vessel that can be replaced by the host and can be unobstructed for a long time is constructed in vivo. The vascular matrix material is prepared according to the above step 1, the vascular matrix material is modified in step 2, and the functional molecule neuropeptide Y (NPY) is coupled according to step 3. , according to step 4 to construct the bioartificial blood vessel in vivo.

[0025] After the bioartificial blood vessel was transplanted to the rat carotid artery, the NPY-modified bioartificial blood vessel prepared by the present invention remained unobstructed after 6 months, while the control group was blocked. HE staining of frozen sections showed no thrombus formation and intimal hyperplasia in blood vessels, but capillaries appeared in adventitia-media. Immunofluorescent staining showed continuous endothelial cells, normal remodeling of smooth muscle cells, and dense and intact extracellular matrix. M...

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Abstract

The invention relates to a bioengineering technology, in particular to a small-caliber biotic artificial blood vessel with anti-thrombus formation and anti-intimal hyperplasia functions. Firstly, the key technology of accellular materials and natural polymer composition is used, and through multiple modes, neurotrophic factors are effectively coupled as functional modules to perform modification on blood vessel supports. Furthermore, through the strategy of constructing in body, the small-caliber engineering blood vessel modified by the neurotrophic factors is transplanted in body, the scour and the pressure of hemodynamics are overcome in body, endothelial progenitor cells in circulation are captured initiatively, the endothelial progenitor cells are further induced to differentiate into endothelial cells in situ, and the progress and the quality of endothelialization of blood vessel graft are speeded. Meanwhile, the generation of blood capillaries is promoted and nurtured, the blood vessel implants are promoted to remodel in body, therefore, the effects of anti-thrombus formation and anti-intimal hyperplasia can be effectively achieved, and the patency rate after the small-caliber biotic artificial blood vessel is transplanted is increased. Active small-caliber tissue engineering blood vessels used for coronary arterie bypass grafting, hematodialysis and cerebrovascular replacement in clinic are built.

Description

technical field [0001] The invention relates to bioengineering technology, in particular to innovative use of neurotrophic factors for modifying bioartificial blood vessels to construct a bioartificial blood vessel with functions of antithrombosis and intimal hyperplasia. Background technique [0002] According to the latest statistics from WHO, the morbidity and mortality of cardiovascular diseases in the world are increasing year by year. Cardiovascular diseases have surpassed tumors to become a serious threat to human health, and 173 million people die of cardiovascular diseases every year. The clinical demand for vascular grafts is increasing worldwide. Peripheral vascular occlusion or damaged replacement, coronary artery bypass grafting, hemodialysis arteriovenous fistula, etc. all require small-caliber vascular grafts. Nowadays, the construction of complex organs such as the liver, kidney, and lung, and the repair of large bone defects require vascularization. The re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/24A61L27/18A61L27/54
Inventor 朱楚洪曾文陈文
Owner 广州宏畅生物科技有限公司
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