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A strain of Durianella and its application

A technology of Duganella and blue pigment, applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of reducing the competitiveness of microbial blue pigment, restricting the development of microbial blue pigment, unfavorable large-scale production of pigment, etc., to facilitate industrial production, Short cycle, less demanding effect

Active Publication Date: 2017-08-25
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purification process of most microbial blue pigments is relatively complicated. The domestically studied blue pigment-producing bacteria are Streptomyces. The production and purification of pigments have brought adverse effects, and at the same time, it is not conducive to the large-scale production of pigments, which reduces the competitiveness of microbial blue pigments, which limits the development of microbial blue pigments

Method used

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  • A strain of Durianella and its application
  • A strain of Durianella and its application
  • A strain of Durianella and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation and identification of Dujania T2013CCTCC M2014643

[0021] 1. Sample collection

[0022] The soil samples were collected from slope soil samples in Zigong City, Sichuan Province.

[0023] 2. Isolation and screening of strains

[0024] Medium preparation:

[0025] (1) Beef extract peptone medium:

[0026] 10g peptone, 3g beef extract, 5g sodium chloride, 15-20g agar, add distilled water to make 1000mL solution, adjust the pH to 7.0-7.2, sterilize with moist heat at 121°C for 20min.

[0027] (2) Potato medium (PDA medium):

[0028] Commercially available potato (peeled) 200g, glucose 20g, agar 15-25g, distilled water 1000mL; pH natural, 121°C moist heat sterilization for 20min.

[0029] (3) Gao's medium I:

[0030] Soluble starch 20g / L, KNO 3 1.0g / L, NaCl 0.5g / L, K 2 HPO 4 ·3H 2 O 0.5g / L, MgSO 4 ·7H 2 O0.5g / L, FeSO 4 ·7H 2 O 0.01g / L; pH 7.2~7.4, sterilized by moist heat at 121℃ for 20min.

[0031] Separation of strains: Take 10g of soil sample and put it in a 250mL E...

Embodiment 2

[0037] Example 2 Preparation of blue pigment with Dujuni T2013CCTCC M2014643

[0038] Strain activation: first put the strains preserved in the frozen glycerol tube into warm water at 50℃ for 30 minutes, then inoculate the thawed strains into the Gao's No. I medium, activate for 24 hours, and then carry out coating treatment and streaking separation. Repeat the plate streak separation operation until the microscopic examination is a pure strain (the cell morphology is consistent).

[0039] Preparation of seed solution: Pick a single colony from the above-mentioned plate-cultured strains and inoculate it into liquid Gaussian No. I medium for 24 hours at 30°C.

[0040] Shake flask fermentation: optimized medium (soluble starch 15g, KNO 3 1.2g, NaCl 0.6g, K 2 HPO 4 ·3H 2 O0.4g, MgSO 4 ·7H 2 O 0.55g, FeSO 4 ·7H 2 O 0.018 g, pH 7.6), 70 mL of culture medium was put into a 250 mL shake flask, the inoculum was 2%, the fermentation temperature was 30° C., the shaker speed was 150 r / min, and...

Embodiment 3

[0050] Example 3 Preparation of blue pigment using Durania T2013CCTCC M2014643

[0051] Strain activation: first put the strains preserved in the frozen glycerol tube into warm water at 50℃ for 30 minutes, then inoculate the thawed strains into the Gao's No. I medium, activate for 24 hours, and then carry out coating treatment and streaking separation. Repeat the plate streak separation operation until the microscopic examination is a pure strain (the cell morphology is consistent).

[0052] Preparation of seed solution: pick a single colony from the above-mentioned plate-cultured strains and inoculate it into liquid Gaussian No. I medium for 24h, 30℃, and shaker rotation speed 180r / min.

[0053] Shake flask fermentation: Optimized medium (soluble starch 15g, KNO3 1.2g, NaCl 0.6g, K2HPO4·3H2O 0.4g, MgSO4·7H2O 0.55g, FeSO4·7H2O 0.018g, pH7.6), fill 70mL of medium Into a 250mL shake flask, the inoculum amount is 5%, the fermentation temperature is 30°C, the shaker rotation speed is 18...

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Abstract

The invention discloses a strain of Dunella and its application. The invention provides Dunella T2013, which is derived from the slope soil of Zigong City, Sichuan Province, and is preserved in the China Center for Typical Culture Collection. The preservation number is Dunella CCTCC M2014643, the fermentation conditions for this strain to produce blue pigment are time 1-7d, liquid volume 30-110ml / 250 ml, inoculum volume 1-7%, pH value 5.5-9, temperature 4-37℃, shake flask speed 30- 210r / min, the fermentation medium per liter contains: soluble starch 5~30g, KNO3 0.6~2.0g, NaCl 0.2~1.5g, K2HPO4 3H2O 0.4~1.2g, MgSO4 7H2O 0.5~0.8 g, FeSO4 7H2O 00.1 ~0.05g, the cyanine produced by this strain is a natural product, without the potential danger of artificial pigments. Studies have proved that the cyanine has good biological activity and will not cause any side effects on animals. It can be widely used in food and cosmetics , feed and pharmaceutical industries.

Description

Technical field [0001] The present invention belongs to the technical field of biochemical industry and colorant biotechnology, and particularly relates to a strain of Dujuni and its application. Background technique [0002] At present, blue pigment is still a rare pigment, and the relevant literature or information is mostly "rare" or "precious" to highlight the importance of developing blue pigment. At present, most of the blue pigments circulating in the market are synthetic blue pigments. Among the natural blue pigments, they are basically plant blue pigments. The production of plant blue pigments is affected by resource factors such as time, space, and environment, and most of them are plant blue pigments. Its application is also affected by factors such as acidity; and microbial blue pigment can make up for the shortcomings of plant blue pigment. Studies have found that microbes can produce blue pigment, and microbial blue pigment has functions such as medicinal or health...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P1/04C12R1/01
CPCC12P1/04C12N1/205C12R2001/01
Inventor 左勇江鹏
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING