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Inactivated vaccine for poultry pathogenic escherichia coli and preparation method thereof

A technology of poultry pathogenic Escherichia coli, which is applied in the field of poultry pathogenic Escherichia coli inactivated vaccine and its preparation, to achieve the effect of preventing infection

Active Publication Date: 2015-07-15
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no commercialized inactivated vaccine against avian pathogenic Escherichia coli O78 serotype in China

Method used

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  • Inactivated vaccine for poultry pathogenic escherichia coli and preparation method thereof
  • Inactivated vaccine for poultry pathogenic escherichia coli and preparation method thereof
  • Inactivated vaccine for poultry pathogenic escherichia coli and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Rejuvenation and identification of candidate strains for seedling production in embodiment 1

[0036] The candidate strain APCE94 for producing seedlings of avian pathogenic Escherichia coli of O78 serotype was obtained through screening in the previous research of the present invention, and the preservation number is CGMCC No.10602.

[0037] After the strain was rejuvenated in susceptible ducks, the strain morphology, virulence factor detection and immune protection rate identification were carried out. The rejuvenated strains were passaged 50 times on LB liquid and LB agar plates, and the morphology of the strains (round raised, smooth, moist, translucent, off-white colonies), biochemical reaction characteristics (fermented lactose and Sorbitol; does not decompose sucrose; does not produce hydrogen sulfide; does not decompose urea; both indole and methyl red tests are positive; VP test and citrate utilization test are negative), molecular biological characteristics (P...

Embodiment 2

[0038] The optimal bacterial content selection of embodiment 2 poultry pathogenic Escherichia coli inactivated vaccine

[0039] 192 10-day-old Cherry Valley ducks were randomly divided into 4 groups, 48 ​​per group, two vaccine immunization test groups and two control groups. The two test groups were randomly divided into 6 subgroups, 10 rats / group, and the immunization dose was 2.5×10 9 CFU / piece, 1×10 9 CFU / piece, 5×10 8 CFU / piece, 2.5×108 CFU / piece, 1×10 8 CFU / piece, 5×10 7 CFU / only and non-immunized control group.

[0040] The vaccines of each group were inoculated subcutaneously in the neck of the corresponding number of Cherry Valley ducks according to the corresponding dose, and the control group did not receive any vaccination treatment. Two weeks later, each group was boosted with the same method once.

[0041] Such as figure 1 As shown, 14 days after the first immunization and the second immunization, the blood was collected from the jugular vein to separate t...

Embodiment 3

[0047] Embodiment 3 prepares O78 serotype avian pathogenic Escherichia coli inactivated vaccine

[0048] O78 serotype APEC inactivated oil emulsion vaccine strain APCE94 was resuscitated, grown, inactivated, and tested as described above, and an inactivated vaccine was prepared.

[0049] The method is: take the bacteria content 3.33×10 9 Add 7 parts of Montanide ISA 70 VG adjuvant or Montanide ISA 71 VG adjuvant to 3 parts of CFU / mL APCE94 inactivated bacteria liquid, mix well, stir and emulsify at high speed for 1 minute / time×2 times to obtain avian pathogenic Escherichia coli Inactivated vaccines. Aliquot 100mL / bottle and store at 4°C.

[0050] The bacterial content of 0.5mL vaccine is 5×10 8 CFU, 0.5mL per mouse was subcutaneously inoculated in the neck during immunization.

[0051] 90 10-day-old unimmunized healthy susceptible Cherry Valley ducks (Qingyang Duck Farm, Wuxi City) were randomly divided into 3 groups, 30 / group, coded as A and B, and group A was given inact...

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Abstract

The invention belongs to the technical field of biology. The invention provides poultry pathogenic escherichia coli strain, the strain is Escherichia coli, and the preservation number is CGMCC 10602. The invention further provides inactivated vaccine for the poultry pathogenic escherichia coli, and the inactivated vaccine for the poultry pathogenic escherichia coli is O 78 serotype poultry escherichia coli inactivate whole bacterial and adjuvant. The immune protective rates of the inactivated vaccine for the poultry pathogenic escherichia coli to the serotype O78 poultry pathogenic escherichia coli virulent strain challenge all reach over 90 percents. The inactivated vaccine for the poultry pathogenic escherichia coli has great immune protective effect on the O 78 serotype poultry escherichia coli strain infection.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to an inactivated vaccine against poultry pathogenic Escherichia coli and a preparation method thereof. Background technique [0002] Avian pathogenic Escherichia coli (APEC) can cause extra-intestinal diseases in chickens, turkeys and other poultry, such as pericarditis, perihepatitis, air sacculitis, meningitis, etc., and even acute death due to sepsis. APEC not only endangers the health of poultry, but also affects the egg production and the time to market of meat poultry, and increases the cost of feeding, causing serious economic losses to the poultry industry. [0003] With the development of my country's poultry industry, avian colibacillosis has become one of the most serious bacterial infectious diseases that endanger the poultry industry. Worldwide, poultry colibacillosis currently causes hundreds of millions of dollars in economic losses to the poultry...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/108A61P31/04C12R1/19
CPCA61K39/0258C12N1/205C12R2001/19
Inventor 于圣青王少辉赵益超韩先干丁铲田明星
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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