A plant source extract for preventing and treating tobacco bacterial wilt
A technology of botanical extracts and tobacco R. solanacearum, applied in the field of botanical pesticides
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Embodiment 1
[0018] Example 1 The inhibitory effect of shepherd's purse ethyl acetate extract and water extract on Ralstonia solanacearum
[0019] Fresh shepherd's purse (including roots, stems, leaves and seeds) is washed, dried in the sun until the water content is 2.5%, and crushed at a speed of 8000-12000r / min to a fine powder of 100-120 mesh. Weigh a quantitative sample of shepherd’s purse dry powder, add 500mL of ethyl acetate, shake at 25-26°C at 100r / min for 36h, filter, collect the filtrate, repeat the extraction of the filter residue once, combine the two filtrates, and place the filtrate at a pressure of 0.09- 0.1Mpa, the temperature of the water bath is 60°C, the condensation temperature is 6°C, and the rotation speed is 80r / min, the rotary evaporator is concentrated to paste, and the ethyl acetate extract is obtained. Dilute the ethyl acetate extract with ethyl acetate and store at 4°C for later use.
[0020] According to the above method, the water extract of shepherd's purs...
Embodiment 2
[0027] Example 2 Inhibition of extracts from different parts of shepherd's purse on tobacco Ralstonia solanacearum
[0028] Take the fresh shepherd's purse root, wash it, dry it in the sun until the water content is 2.5%, and grind it to a fine powder of 100-120 mesh at a speed of 10000r / min. Weigh quantitative shepherd's purse root dry powder, add 400mL ethyl acetate, shake in a shaking table for 36h, and collect the filtrate; filter residue is repeatedly extracted once, combined with 2 filtrates, put into a rotary evaporator to concentrate to obtain a paste, and obtain the extract of shepherd's purse root. Dilute to 20 mL with ethyl acetate. According to the same method, the ethyl acetate extracts of shepherd's purse leaves, stems and seeds were prepared respectively.
[0029] The antibacterial effect of extracts from different parts of shepherd's purse on R. solanacearum was determined by the zone of inhibition method. The sterilized NA medium was poured into a petri dish ...
Embodiment 3
[0034] Example 3 Extraction efficiency of extracts from different parts of shepherd's purse
[0035] Take the fresh shepherd's purse root, wash it, dry it in the sun until the water content is 2.5%, and grind it to a fine powder of 100-120 mesh at a speed of 10000r / min. Take by weighing 20g shepherd's purse root powder, according to the method in embodiment 2, take ethyl acetate as extraction solvent, prepare shepherd's purse root extract paste. Utilize subtraction method to calculate paste weight, calculate the extraction rate of shepherd's purse root extract according to dry powder quality and paste quality. According to the above extraction method, the ethyl acetate extract paste of shepherd's purse stem, leaf and seed was respectively obtained, and the extraction rate was calculated. The results are shown in Table 3 below.
[0036]
[0037] The results showed that the extraction rate of shepherd's purse leaf was the highest at 15.2%, followed by the extraction rate of ...
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