Angiotensin converting enzyme detection kit with good repeatability

An angiotensin and detection kit technology, which is applied in the direction of analysis by chemical reaction of materials, material analysis by observing the influence on chemical indicators, etc., can solve unfavorable detection results, judgment, poor repeatability, etc. problem, to solve the problem of poor repeatability, reduce surface tension, and reduce surface free energy.

Inactive Publication Date: 2015-07-22
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the poor repeatability of common angiotensin-converting enzyme detection reagents, the accuracy of the reagent will be affected, which is not conducive to the judgment of the test results, resulting in the consequences of misdiagnosis

Method used

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  • Angiotensin converting enzyme detection kit with good repeatability
  • Angiotensin converting enzyme detection kit with good repeatability
  • Angiotensin converting enzyme detection kit with good repeatability

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Component R:

[0025] FAPGG0.5mmol / L

[0026] Sodium chloride 300 mmol / L

[0027] Borate buffer (PH 8.2) 80mM

[0028] The angiotensin-converting enzyme detection kit (colorimetric method) described in this embodiment, when in use, its assay method is to use an automatic biochemical analyzer, such as Toshiba 120 automatic analyzer, the operation is as shown in the figure:

[0029] .

Embodiment 2

[0031] Component R:

[0032] FAPGG0.5mmol / L

[0033] Sodium chloride 300 mmol / L

[0034] Borate buffer (PH 8.2) 80mM

[0035] AEO-9 0.1%

[0036] The detection method is as in Example 1.

Embodiment 3

[0038] Component R:

[0039] FAPGG0.5mmol / L

[0040] Sodium chloride 300 mmol / L

[0041] Borate buffer (PH 8.2) 80mM

[0042] AEO-9 0.5%

[0043] Concrete determination method is with embodiment 1.

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Abstract

The invention provides an angiotensin converting enzyme detection kit with good repeatability. The detection kit comprises the following components: 0.5 mmol / L of FAPGG, 300 mmol / L of NaCl, 80 mM of a borate buffer solution with the PH value of 8.2 and 0.1-1% of AEO-9. The detection kit provided by the invention overcomes the defect of poor repeatability in the prior art in detecting angiotensin converting enzyme.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to an angiotensin converting enzyme detection kit. Background technique [0002] Angiotensin-converting enzyme (Angiotensin-Converting Enzyme, ACE, EC 3.4.15.1, named peptidyl dipeptide hydrolase systematically), is a membrane-bound exopeptidase (carboxyl Terminal dipeptide), which catalyzes the hydrolysis of angiotensin Ⅰ to generate angiotensin Ⅱ with vasoconstriction or hydrolyzes bradykinin with vasoconstriction to generate phenylpropanoid-sperm dipeptide, which is widely used in the food and pharmaceutical industries. Since 1898 Since the discovery of renin, studies on the renin-angiotensin system (RAS or renin-angiotensin-aldosterone system, RAAS) and the kallikrein-kinin system (Kallikrein-Kinin System, KKS) have been For more than a hundred years, these two systems mainly play an indirect role through the control of blood pressure, electrolytes,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 谭柏清董雯甘宜梧王绮包兴艳肖慧
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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