Counting detection method of bacillus coagulans

A technology of Bacillus coagulans and a detection method, which is applied in the detection field of Bacillus coagulans vegetative bodies and spores in Bacillus coagulans bacteria liquid or bacteria powder, can solve the problem that the medium components and culture conditions are not provided, and the bacterial dispersion cannot be completely solved. problems such as speed, unfavorable actual production, etc., to achieve the effect of reporting time in advance, shortening the scientific research cycle, and preparing results

Active Publication Date: 2021-08-10
长沙和光生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this patent has the following deficiencies: (1) After the water bath heating is placed in the homogenization, it is very easy to cause the dispersed bacteria to settle under the static heating environment and then re-agglomerate, resulting in deviations in the counting results
(2) Use physiological saline containing Tween 80 to shake and disperse bacterial powder in a triangular flask, which is suitable for dispersing bacteria with low cohesion, but cannot completely solve the problem of bacterial dispersion under strong cohesion.
(3) As we all know, the growth of bacteria requires suitable medium components and culture conditions. This patent does not provide medium components and culture conditions that are widely applicable to various Bacillus coagulans strains, and the scope of application is narrow
(4) The patent needs to be cultivated for 48±2h to calculate the results, and the detection cycle is long, which is not conducive to actual production

Method used

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  • Counting detection method of bacillus coagulans
  • Counting detection method of bacillus coagulans
  • Counting detection method of bacillus coagulans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Bacillus coagulans powder spore content counting detection, said Bacillus coagulans powder is a product of a domestic company, and the content indicated on the package is 100×10 8 CFU / g, referred to as sample No. 1 in the present invention.

[0036] 1) Preparation of sterile diluent. Accurately weigh 4.5g of sodium chloride, 4g of sodium lauryl sulfate and 1g of Tween 60, fully dissolve them in deionized water and dilute to 500mL, put them in a 1L Erlenmeyer flask, sterilize at 121°C for 15min, cool Set aside at room temperature.

[0037] 2) BC plate preparation. Accurately weigh 3g of tryptone, 1.8g of yeast extract, 1.8g of glucose, 1.8g of beef extract, 0.45g of sodium acetate, 0.3g of dipotassium hydrogen phosphate, 0.09g of L-cysteine ​​hydrochloride, anhydrous chlorine Calcium chloride 0.06g, manganese sulfate monohydrate 0.03g, use a small amount of hot deionized water to fully dissolve and then adjust the volume to 300mL, use phosphoric acid to adjust the pH ...

Embodiment 2

[0049] Other conditions are the same as in Example 1, except that the amount of sodium lauryl sulfate in step 1) is 6g, and the amount of Tween 60 is 1.5g. After the BC medium in step 2) is prepared to constant volume, use phosphoric acid to adjust the pH to 5.5, the test results are shown in Table 2 below:

[0050] Table 2

[0051]

Embodiment 3

[0053] Other conditions are identical with embodiment 1, and difference is that step 1) sodium lauryl sulfate consumption is 6g, and Tween 60 consumption is 1g, and detection result is as shown in table 3 below:

[0054] table 3

[0055]

[0056]

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Abstract

The invention relates to a counting detection method of bacillus coagulans. The counting detection method comprises the following steps: (S1) carrying out the following operations on a sample to be detected through sterile operation: performing diluting, thermal activating, ultrasonic dispersing and homogenizing to obtain a sample diluent; (S2) carrying out continuous gradient dilution on the sample diluent until the estimated bacterium content of the sample diluent is 10-300CFU/mL, and ending dilution; (S3) culturing a sample with a proper dilution gradient in a BC culture medium with the pH value of 5.0-5.5, and counting bacterial colonies of bacillus coagulans. The method can be widely applied to detection of different bacillus coagulans strains, the counting result is accurate, the average error is small, and the result can be obtained within the detection period of only 26-30 hours.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to a detection method for bacillus coagulans vegetative bodies and spores in bacillus coagulans bacteria liquid or bacteria powder. Background technique [0002] Bacillus coagulans (Bacillus coagulans) is currently the only spore-forming lactic acid bacteria that has been approved by the governments of various countries to add to food and feed. Its main beneficial function is to produce lactic acid during intestinal metabolism, which is very important for promoting digestion and absorption and maintaining the balance of intestinal flora. At the same time, because its spores have excellent stress resistance properties such as high temperature resistance, acid and alkali resistance, and bile salt resistance, the application range of probiotics in the food industry has been greatly expanded. In recent years, Bacillus coagulans has attracted the attention of many food an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12R1/07
CPCC12Q1/06C12Q1/045G01N2333/32
Inventor 刘笑尘李鑫
Owner 长沙和光生物科技有限公司
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