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Glutamate dehydrogenase detection kit

A technology of glutamate dehydrogenase and detection kit, which is applied in the direction of measuring device, color/spectral characteristic measurement, and material analysis by observing the influence on chemical indicators, etc., which can solve the problem of poor repeatability and poor accuracy Waste reagents and other problems, to achieve the effect of solving poor repeatability, reducing surface free energy, and reducing surface tension

Inactive Publication Date: 2018-03-09
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, common glutamate dehydrogenase detection reagents have poor repeatability, resulting in poor accuracy and waste of reagents.

Method used

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  • Glutamate dehydrogenase detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A glutamate dehydrogenase kit with excellent accuracy recognized on the market, which includes reagent R1, reagent R2, and calibrator.

[0023] Wherein the reagent R1 consists of:

[0024] Tris buffer 100mmol / L

[0025] α-ketoglutarate 10mmol / L

[0026] Sodium azide 0.5% (W / V)

[0027] Reagent R2 consists of:

[0028] Tris buffer 100mmol / L

[0029] Ammonium chloride 100mmol / L

[0030] Reduced coenzyme Ⅰ 0.5mmol / L

[0031] Sodium Azide 0.5% (W / V).

[0032] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0033] Add 15 μl of physiological saline, sample or calibrator, then add 240 μl of R1 reagent for pre-incubation for 5 minutes, then add 60 μl of reagent R2, start recording the absorbance value A1 after 30 seconds, and read the absorbance A2 after 5 minutes of reaction, and calculate ΔA / min.

[0034] The calibrator used in this embodiment is...

Embodiment 2

[0036] A glutamate dehydrogenase detection kit, which comprises reagent R1, reagent R2 and calibrator.

[0037] Wherein the reagent R1 consists of:

[0038] Tris buffer 100mmol / L

[0039] α-ketoglutarate 10mmol / L

[0040] Sodium azide 0.5% (W / V)

[0041] Reagent R2 consists of:

[0042] Tris buffer 100mmol / L

[0043] Ammonium chloride 100mmol / L

[0044] Reduced coenzyme Ⅰ 0.5mmol / L

[0045] AEO-9 0.1% (V / V)

[0046] Sodium azide 0.5% (W / V)

[0047] Concrete determination method is with embodiment 1.

Embodiment 3

[0049] A glutamate dehydrogenase detection kit, which comprises reagent R1, reagent R2 and calibrator.

[0050] Wherein the reagent R1 consists of:

[0051] Tris buffer 100mmol / L

[0052] α-ketoglutarate 10mmol / L

[0053] Sodium azide 0.5% (W / V)

[0054] Reagent R2 consists of:

[0055] Tris buffer 100mmol / L

[0056] Ammonium chloride 100mmol / L

[0057] Reduced coenzyme Ⅰ 0.5mmol / L

[0058] AEO-9 0.5% (V / V)

[0059] Sodium azide 0.5% (W / V)

[0060] Concrete determination method is with embodiment 1.

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Abstract

The invention discloses a glutamate dehydrogenase detection kit, and belongs to the technical field of clinical in-vitro detection reagents. The kit comprises a reagent R1, a reagent R2 and a calibration product. By adding of AEO (alkyl polyoxyethylene ether)-9 into the reagent R2, the problem of low repetitiveness of the reagent is solved; the glutamate dehydrogenase detection kit is relatively wide in linearity range, high in reagent accuracy, and favorable for being further popularized and used in the market.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to a glutamate dehydrogenase detection kit. Background technique [0002] Glutamate dehydrogenase (GLDH or GDH) is an enzyme that mainly exists in the mitochondrial matrix of cells, with the highest content in the liver, followed by kidneys, pancreas, brain, small intestinal mucosa, and heart. Its determination method is mainly continuous monitoring method. [0003] The activity of GLDH in the blood of normal people is very low, and the reference value range is: 0-12.2U / L. It enters the blood when liver cell necrosis occurs. Therefore, the activity of GLDH increases significantly in liver diseases, especially when liver cell mitochondria are damaged, so it is commonly used It is a sensitive indicator of liver parenchyma damage. The activity of GLDH in the serum increases significantly in alcohol poisoning with hepatic necrosis, chronic hepatitis, hepat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/3103G01N21/78
Inventor 甘宜梧胡晓飞谭柏清董雯王美丽
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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