A kind of hplc-esi-ms/ms method detects the method for multiple quinolone drug residues in feed
A quinolone and feed technology, applied in the field of analysis and testing, can solve problems such as affecting the clinical efficacy of drugs, and achieve the effects of simple and fast sample pretreatment method, shortened detection time, and shortened pretreatment time.
Active Publication Date: 2017-02-08
SHANDONG ANALYSIS & TEST CENT
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Problems solved by technology
[0003] Long-term human consumption of animal foods and Chinese patent medicine health foods containing low concentrations of quinolones can easily induce the transmission of drug resistance, thereby affecting the clinical efficacy of such drugs
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[0024] 1.1 Instruments and reagents
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The invention discloses a method for detecting residue of multiple quinolones in a feed by adopting an HPLC-ESI-MS / MS process. A normal-temperature ultrasonic method is adopted for extracting multiple quinolones in a feed sample at the same time, and a method for detecting residue of multiple quinolones in the feed through high performance liquid chromatography-triple quadrupole mass spectrometry is established, sample pre-treatment time can be greatly reduced, and recovery rate and repeatability of the detection method can meet analytical requirements of veterinary medicines in the feed, wherein the recovery rate is up to more than 95%, RDS is less than 6%, and detection limit can be up to 50mu g / kg. The method for detecting residue of multiple quinolones in the feed by adopting the HPLC-ESI-MS / MS process has the advantages that operation is simple, detection sensitivity is high, results are reliable, and rapid and low-cost detection of residue of eight quinolones can be realized.
Description
technical field [0001] The invention belongs to the field of analysis and testing, in particular to a method for detecting multiple quinolone drug residues in feed by an HPLC-ESI-MS / MS method. Background technique [0002] Quinolones, also known as pyroxic acids or pyridonic acids, are synthetic antibacterial drugs containing the basic structure of 4-quinolone. Commonly used drugs are norfloxacin, dafloxacin, ciprofloxacin and so on. These drugs have bactericidal effect on a variety of Gram-negative bacteria, and are widely used in the treatment of genitourinary system diseases, gastrointestinal diseases, and Gram-negative bacterial infections of the respiratory tract and skin tissue. With the rapid development of my country's animal husbandry and the frequent occurrence of various animal diseases caused by the continuous deterioration of the global ecological environment, farmers have begun to use a large number of drugs to prevent diseases or as feed additives to promote ...
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IPC IPC(8): G01N30/02
Inventor 王珊珊赵汝松王晓利宁凡盛苑金鹏陈相峰陈跃李磊张莉莉赵金
Owner SHANDONG ANALYSIS & TEST CENT