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a use of co 2 Methods of growing microalgae

A CO2 and microalgae technology, applied in the direction of single-cell algae, etc., can solve the problems of low CO2 utilization rate, decreased microalgae biomass yield, and toxic microalgae cells, so as to achieve high CO2 utilization rate and increase biomass production rate, no toxic effects

Active Publication Date: 2018-02-16
INST OF PROCESS ENG CHINESE ACAD OF SCI
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Problems solved by technology

Both of them mostly pass CO2 into the culture medium by bubbling. 2 gas as a carbon source, but the problem with this method is that CO 2 The absorption rate is low, resulting in CO 2 Low utilization rate and high cost of carbon source
Although this method can increase the total inorganic carbon source concentration in the culture solution under near-neutral (pH6-8) conditions, high concentrations of monoethanolamine (>2mmol / L) are toxic to microalgal cells, which in turn leads to Biomass production decreased (Sun Z, Zhang D, Yan C, et al. Promotion of microalgal biomass production and efficient use of CO 2 from flue gas by monoethanolamine[J].Journal of Chemical Technology and Biotechnology,2015,90(4):730-8)

Method used

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  • a use of co <sub>2</sub> Methods of growing microalgae
  • a use of co <sub>2</sub> Methods of growing microalgae
  • a use of co <sub>2</sub> Methods of growing microalgae

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Experimental program
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Effect test

Embodiment 1

[0038] A columnar glass photobioreactor (height 50cm, inner diameter 6cm) is used indoors to cultivate microalgae, and aeration stones commonly used in this field (aperture 30-60μm) are used to provide a light intensity of 100μmol·m from 8 fluorescent tubes. -2 ·s -1 The 12h:12h (light:dark) of the light. The algal species is Scenedesmus dimorphus, which comes from the freshwater algae species bank of the Institute of Hydrobiology, Chinese Academy of Sciences, number 496. Scenedesmus dimorphus was cultured with BG11 as the medium, and the culture volume was 1L. When preparing the medium, Tris was added to a final concentration of 2 mmol / L, the medium was sterilized at 121°C and 0.1 MPa for 20 minutes, and microalgae were added after cooling. Use an air conditioner to control the room temperature at 25°C, and continuously feed air to drive the culture medium to mix for 24 hours. The air flow rate is 180mL / min. During the period of 12 hours of illumination, pure CO is fed ever...

Embodiment 2

[0049] Others are the same as in Example 1, except that Tris is added to the medium to a final concentration of 4mmol / L.

[0050] Adding Tris with a final concentration of 4mmol / L in the culture medium, the average total inorganic carbon source concentration in the culture medium during the culture was 1.44mmol / L; while using the same reactor and the same culture conditions to cultivate the same microalgae, only Without adding Tris, the average total inorganic carbon source concentration of the culture medium was 0.53mmol / L, such as figure 1 shown.

[0051] Adding Tris with a final concentration of 4mmol / L in the medium, the average biomass production rate of batch culture for 8 days was 0.15g / L / d; while using the same reactor and the same culture conditions to cultivate the same microalgae, Just without adding Tris, the average biomass yield obtained by batch culture for 8 days was 0.097g / L / d, such as figure 2 shown.

[0052]Adding Tris with a final concentration of 4mmol...

Embodiment 3

[0059] Others are the same as in Example 1, except that Tris is added to the medium to a final concentration of 6 mmol / L.

[0060] Adding Tris with a final concentration of 6mmol / L in the culture medium, the average total inorganic carbon source concentration in the culture medium during the culture was 1.67mmol / L; while using the same reactor and the same culture conditions to cultivate the same microalgae, only Without adding Tris, the average total inorganic carbon source concentration of the culture medium was 0.53mmol / L, such as figure 1 shown.

[0061] Adding Tris with a final concentration of 6mmol / L in the medium, the average biomass production rate of batch culture for 8 days was 0.16g / L / d; while using the same reactor and the same culture conditions to cultivate the same microalgae, Just without adding Tris, the average biomass yield obtained by batch culture for 8 days was 0.097g / L / d, such as figure 2 shown.

[0062] Adding Tris with a final concentration of 6mm...

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Abstract

The invention belongs to the field of microalgae cultivation, and particularly relates to a method for cultivating microalgae by using CO2 as a carbon source. Tris is added into a microalgae cultivating solution containing the CO2 gas serving as the carbon source, so that the concentration of the total inorganic carbon source in the cultivation solution is obviously increased, and the biological yield of the microalgae is increased. Particularly in the cultivation solution in which the macroalgae with the pH value of 6-8 grows properest, according to the method, higher CO2 utilization rate can be guaranteed; meanwhile, the added Tris cannot be consumed by microalgae cells, so that a repeated carbon fixing effect can be achieved, and the economic property is improved. Compared with mono ethanol, Tris nearly does not have a harm effect on the microalgae; when the adding final concentration is 2-8 mmol / L, the biomass yield is increased; furthermore, when the adding final concentration is 4-6 mmol / L, the increase degree is the largest; meanwhile, the biochemical composition of the microalgae cells cannot be affected by the Tris.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, in particular to a 2 As a method for cultivating microalgae as a carbon source, it particularly relates to a method for utilizing CO under near-neutral (pH6-8) culture conditions. 2 A method of culturing microalgae as a carbon source. Background technique [0002] Microalgae are a class of photoautotrophic microorganisms that can fix CO 2 Production of various chemicals. Some can produce aliphatic hydrocarbons, such as botrytis, which can produce hydrocarbons up to 15% to 75% of dry cell weight, some can accumulate glycogen, and some can accumulate glycerol, and the oil content of many microalgae can reach 60% of dry weight. %above. Because of its fast growth rate and the ability to use waste water and waste gas for cultivation, it has received more and more attention worldwide. [0003] At present, the culture methods of microalgae are mainly divided into open culture and closed cultur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12
CPCC12N1/12
Inventor 丛威孙中亮颜成虎薛升长温树梅张东梅
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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