Kit for simultaneously detecting content of each of TPSII, SSA, hs-CRP, PCT and resistin in blood plasma and application of kit

A test kit and resistin technology, applied in the biological field, can solve the problem of no and early investigation, while in China, it has only begun to enter clinical applications and reports in tertiary hospitals in the past two years, so as to save consumption. The effect of saving labor and solving collection difficulties

Active Publication Date: 2015-08-05
苏州菩罗莛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the detection of urinary trypsinogen-2 in patients with acute abdominal pain is also used to screen for acute pancreatitis, but there is no report on the relevant biomarkers for the screening of other diseases related to acute abdominal pain
In China, the clinical detection of acute abdominal pain is similar to that of foreign clinical detection, but the application of TPSⅡ in foreign clinical diagnosis was carried out earlier, but in China, it has only started to enter clinical application in tertiary hospitals in the past two years. The clinical application value is still in further exploration

Method used

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  • Kit for simultaneously detecting content of each of TPSII, SSA, hs-CRP, PCT and resistin in blood plasma and application of kit
  • Kit for simultaneously detecting content of each of TPSII, SSA, hs-CRP, PCT and resistin in blood plasma and application of kit
  • Kit for simultaneously detecting content of each of TPSII, SSA, hs-CRP, PCT and resistin in blood plasma and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation of serum TPSⅡ, hs-CRP, SSA, PCT and resistin immunoturbidimetric assay kits.

[0035] A kit for simultaneous determination of serum TPSII, hs-CRP, SSA, PCT and resistin, the kit includes the following components: reagent one (R1), which contains phosphate buffer and polyethylene glycol; reagent two ( R2), which contains phosphate buffer, sensitized latex coupled with anti-TPSII monoclonal antibody a (hereinafter referred to as monoclonal antibody a), coupled with anti-hs-CRP monoclonal antibody b (hereinafter referred to as monoclonal antibody b) the sensitized latex, the sensitized latex stabilizer linked with anti-SSA monoclonal antibody c (hereinafter referred to as monoclonal antibody c), and the sensitized latex stabilizer linked with anti-PCT monoclonal antibody d (hereinafter referred to as monoclonal antibody d) Sensitive latex, sensitized latex combined with anti-resistin monoclonal antibody e (hereinafter referred to as monoclonal antibod...

Embodiment 2

[0055] Example 2 Accuracy of Serum TPSⅡ, hs-CRP, SSA, PCT and Resistin Double Latex Enhanced Immunoturbidimetric Assay Kit

[0056] (1) Accuracy and precision

[0057] Choose high, medium and low concentrations of standard products, each containing TPSⅡ, hs-CRP, SSA, PCT and resistin at a concentration of 500ng / ml, 200ng / ml, 450ng / ml, 450ng / ml and 300ng / ml, 200ng / ml, 150ng / ml, 180ng / ml, 170ng / ml and 140ng / ml, 50ng / ml, 30ng / ml, 100ng / ml, 70ng / ml and 75ng / ml. The latex-enhanced immune turbidimetry detection kit designed by the present invention is used to carry out the above-mentioned samples, and each concentration is detected 10 times, and the obtained results are compared to determine the accuracy and precision of the kit. The obtained detection results are shown in Table 2 :

[0058] Table 2. Comparison of test results of TPSⅡ, hs-CRP, SSA, PCT and resistin

[0059]

[0060] The T-test of paired samples was performed on the data of this group, and P>0.05 was obtained,...

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Abstract

The invention discloses a kit for simultaneously detecting the content of each of TPSII, SSA, hs-CRP, PCT and resistin in a blood plasma and the application of the kit. The kit comprises a detection solution R1, a detection solution R2, an anti-biomarker positive quality control blood serum, an anti-biomarker negative quality control blood serum and a concentrated washing liquid; the content of each of trypsase TPSII, SSA, hs-CRP, PCT and resistin in the human blood plasma is detected together, so that the inspection of acute stomachache inducing diseases such as acute pancreatitis, celiac viscera inflammatoryb lesions and acute peptic ulcer piercing can be realized; the detection method of the it is based on latex-enhanced Immunoturbidimetry, and therefore, multi-index combined detection is realized and the purposes of identifying common diseases causing acute stomachache is achieved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for simultaneously measuring trypsinogen-2 (TPSⅡ), serum amyloid A (SSA), and hypersensitive C-reactive protein (hs-CRP) in plasma by using a latex-enhanced immunoturbidimetric method , procalcitonin (PCT) and resistin content detection kits, realize the investigation of common diseases of acute abdominal pain, and provide auxiliary information for clinicians to make timely and reasonable diagnoses. Background technique [0002] Acute abdominal pain is one of the most common complaints in emergency department patients. The visits can occur throughout the year, with peaks in autumn (September-November) and summer (June-August), and the age of visits is concentrated between 15 and 44 years old (accounting for 72.7% of the total visits). It often involves internal, external, pediatric, obstetrics and even neurological and psychiatric diseases. The main etiology is the lesions...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893
Inventor 杨子学董梅
Owner 苏州菩罗莛生物科技有限公司
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