Anti-inflammatory application of pomegranate flower polyphenol

A technology of pomegranate flower polyphenols and uses, which is applied in the field of biomedicine and can solve the problems of no report on the anti-inflammatory effect of pomegranate flower polyphenols and little research on the biological activity of pomegranate flower.

Inactive Publication Date: 2015-08-12
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, scientific researchers have conducted a lot of research on pomegranate juice, pomegranate seed oil, pomegranate peel extracts and derivatives. However, the research on pomegranate flower is mostly focused on its chemi

Method used

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  • Anti-inflammatory application of pomegranate flower polyphenol
  • Anti-inflammatory application of pomegranate flower polyphenol
  • Anti-inflammatory application of pomegranate flower polyphenol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Biological activity screening of pomegranate flower polyphenols as anti-inflammatory drugs or health products:

[0016] Determination of cell viability by MTT colorimetry: RAW264.7 macrophages in the logarithmic growth phase were seeded in 96-well culture plates at a cell density of 1×10 4 cells / mL, 100 μL per well, temperature 37°C, 5% CO 2 After culturing overnight under the same conditions, pomegranate flower polyphenols (10-100 μg / mL) solutions of different concentrations were added to investigate the effect of the drug on the cells. After the cells of the above drug groups were cultured overnight, 5 mg / ml mL MTT 20μL, temperature 37℃, 5%CO 2 After continuing to incubate for 4 h under the same conditions, the culture was terminated, the liquid in the well was discarded, 100 μL of dimethyl sulfoxide (DMSO) was added to each well, and shaken for 10 min to fully dissolve the intracellular crystals, and the microplate reader was used to measure the concentration of eac...

Embodiment 2

[0018] Biological activity screening of pomegranate flower polyphenols as anti-inflammatory drugs or health products:

[0019] Determination of nitric oxide (NO) content by Griess method: To investigate the inhibitory effect of pomegranate flower polyphenols on the inflammatory response of mouse macrophage RAW264.7 induced by lipopolysaccharide. Mouse macrophage RAW264.7 cells were subcultured in high-glucose cell medium DMEM (dulbecco's minimum essential medium, DMEM, Gibico) containing 10% fetal bovine serum (FBS), and different concentrations of pomegranate flower polyphenols were added (10-100 μg / mL), bacterial lipopolysaccharide (1 μg / mL) induced an inflammatory response, and the supernatant was collected after 16 hours. The content of nitric oxide (NO) in the cell supernatant was determined by Griess method, and the effect of different concentrations on the release of nitric oxide (NO) from RAW 264.7 cells induced by bacterial lipopolysaccharide was used to reflect the l...

Embodiment 3

[0021] Biological activity screening of pomegranate flower polyphenols as anti-inflammatory drugs or health products:

[0022] Determination of interleukin-6 (Interleukin-6, IL-6) by ELISA: RAW264.7 macrophages in the logarithmic growth phase were seeded in 24-well culture plates at a cell density of 1×10 5 pcs / ml, 1mL per well, temperature 37℃, 5%CO 2 conditions, the pomegranate flower extract group (final concentrations of 10, 25, 50, 100 μg·mL -1 ), on the basis of the above drug group, add bacterial lipopolysaccharide (final concentration: 1 μg / mL) to each well, first add the drug and incubate for 1 h, then add bacterial lipopolysaccharide, stimulate the cells and incubate for 16 h, repeat each group 3 holes. The content of IL-6 cytokines secreted by RAW264.7 macrophages treated with pomegranate flower polyphenols was determined by ELISA ( image 3 ).

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Abstract

The invention relates to an anti-inflammatory application of pomegranate flower polyphenol. An enzyme-linked immunosorbent assay (ELISA) technology is adopted to analyze the influence of pomegranate flower polyphenol on the lipopolysaccharide-induced inflammatory response of mouse macrophage (RAW264.7), and the results show that pomegranate flower polyphenol can prominently inhibit the excess releasing of inflammatory mediator namely nitrogen monoxide (NO) and excess secretion of inflammation inhibiting factor namely interleukin 6, interleukin 1[beta], and tumor necrosis factor [alpha]. The western blotting shows that pomegranate flower polyphenol can inhibit the excess expression of the following three key enzymes in the inflammation signal path: extracelluar regulated protein kinase, C-JUN N-terminal kinase, and epoxidase 2. So the pomegranate flower polyphenol has a very good anti-inflammatory activity, can be used as the lead compound of anti-inflammatory drugs to prevent or treat inflammation caused by excess of nitrogen monoxide (NO), tumor necrosis factor [alpha] (TNF-[alpha]), interleukin 6 (IL-6) or interleukin 1[beta] (IL-1[beta]).

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the use of pomegranate flower polyphenols, a medicinal material commonly used in Uighur medicine, in the preparation of anti-inflammatory drugs or health care products. Background technique [0002] Inflammation is a common clinical pathological process that can occur in various tissues and organs of the body. When the body encounters a pathogen attack, it will release a series of cytokines to attract immune cells to the site of infection and cause inflammation. Inflammation can thus be said to be a defense mechanism that the body uses to fight infection. But uncontrolled inflammation can also cause a series of pathological reactions, such as severe infection and sepsis, Alzheimer's disease, atherosclerosis and type 2 diabetes. Macrophages are the body's first line of immune defense. Once the body is injured, macrophages can secrete a large number of macrophage-derived biological fact...

Claims

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Application Information

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IPC IPC(8): A61K36/185A23L1/29A61P29/00A23L33/00
Inventor 汤丹赵永昕信学雷阿吉艾克拜尔·艾萨
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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