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Purification process of L-tryptophan

A tryptophan and process technology, applied in the field of L-tryptophan purification technology, can solve the problem of low L-tryptophan purity, and achieve the effects of simple process control, good decolorization and impurity removal effects

Inactive Publication Date: 2015-08-12
南通荣泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the purity of L-tryptophan prepared is not high. Therefore, it is necessary and urgent to develop a purification process for L-tryptophan to improve the purity of the product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A purification process for L-tryptophan, comprising the following steps:

[0021] Step 1) Decolorization, pour distilled water into the dissolving tank, and start stirring. The speed of the mixer is 60r / min, and the stirring time is 60 minutes. Open the steam inlet and outlet valves. When the temperature rises to 40°C, put in the crude product with a purity of 98% and Activated carbon, the mass ratio of crude product and activated carbon is 20:1, continue heating to 90 ℃;

[0022] Step 2) Filtration, first rinse the filter bucket with distilled water, clean the filter cloth on the filter bucket with distilled water, put the product obtained in step 1 into the crystallization tank, open the vacuum valve of the crystallization tank for suction filtration, the speed of suction filtration It is 1000L / H, and the time is about 1.5h. After the suction filtration is completed, immediately send cooling, and when the temperature of the crystallization tank drops to 12°C, sto...

Embodiment 2

[0029] A purification process for L-tryptophan, comprising the following steps:

[0030] Step 1) Decolorization, pour distilled water into the dissolving tank, and start stirring. The speed of the mixer is 60r / min, and the stirring time is 60 minutes. Open the steam inlet and outlet valves. When the temperature rises to 42°C, put in the crude product with a purity of 98% and Activated carbon, the mass ratio of crude product and activated carbon is 20:1, continue heating to 93 ℃;

[0031] Step 2) Filtration, first rinse the filter bucket with distilled water, clean the filter cloth on the filter bucket with distilled water, put the product obtained in step 1 into the crystallization tank, open the vacuum valve of the crystallization tank for suction filtration, the speed of suction filtration It is 1000L / H, and the time is about 1.5h. After the suction filtration is completed, immediately send cooling, and when the temperature of the crystallization tank drops to 14°C, stop...

Embodiment 3

[0038] A purification process for L-tryptophan, comprising the following steps:

[0039] Step 1) Decolorization, pour distilled water into the dissolving tank, and start stirring. The speed of the mixer is 60r / min, and the stirring time is 60 minutes. Open the steam inlet and outlet valves. When the temperature rises to 45°C, put in the crude product with a purity of 98% and Activated carbon, the mass ratio of crude product and activated carbon is 20:1, continue heating to 95 ℃;

[0040] Step 2) Filtration, first rinse the filter bucket with distilled water, clean the filter cloth on the filter bucket with distilled water, put the product obtained in step 1 into the crystallization tank, open the vacuum valve of the crystallization tank for suction filtration, the speed of suction filtration It is 1000L / H, and the time is about 1.5h. After the suction filtration is completed, immediately send cooling, and when the temperature of the crystallization tank drops to 15°C, stop...

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Abstract

The invention discloses a purification process of L-tryptophan. The purification process comprises the following steps: decoloring, filtering, centrifuging, drying, mixing and weighing for packaging. The purity of the product obtained by the process is more than 99%, the light transmittance of the product is about 98.5, the ferric salt of the product is lower than 10ppm, the heavy metal of the product is lower than 10ppm, and a mother solution can be reused for multiple times. The preparation process is simple in overall process control, is relatively convenient, and accords with a national standard of L-tryptophan.

Description

technical field [0001] The invention belongs to the field of bioengineering, and specifically relates to a process for purifying L-tryptophan. Background technique [0002] Tryptophan, also known as α-amino-β-indolepropionic acid, has a molecular weight of 204.23. L-Tryptophan is white or slightly yellowish leaf-like crystal or powder, odorless or slightly smelly. Soluble in boiling water, hot ethanol, slightly soluble in water. L-Tryptophan is one of the essential amino acids in human and animal life activities. It plays an important role in the growth and development and growth metabolism of humans and animals. It is widely used in medicine, food and feed, etc. At present, the production methods of L-tryptophan include chemical synthesis method, proteolysis method, microbial transformation method, enzymatic method and direct fermentation method, among which direct fermentation method is the mainstream method of tryptophan production at present. [0003] At present, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D209/20
CPCC07D209/20
Inventor 曹嵘
Owner 南通荣泰生物科技有限公司