Method for synchronously extracting sargassum graminifolium polysaccharide and sargassum graminifolium polyphenol from sargassum graminifolium

Abstract

The invention relates to a method for synchronously extracting sargassum graminifolium polysaccharide and sargassum graminifolium polyphenol from sargassum graminifolium. The method for synchronously extracting the sargassum graminifolium polysaccharide and the sargassum graminifolium polyphenol from the sargassum graminifolium is characterized in that microwave-assisted extraction, two aqueous phase extraction and membrane separation techniques are applied for extracting the sargassum graminifolium polysaccharide and the sargassum graminifolium polyphenol from the sargassum graminifolium at the same time. The invention also provides the sargassum graminifolium polysaccharide and the sargassum graminifolium polyphenol which are obtained by adopting the method provided by the invention. The method for synchronously extracting the sargassum graminifolium polysaccharide and the sargassum graminifolium polyphenol from the sargassum graminifolium has the advantages that sargassum graminifolium polysaccharide extraction ratio is high, biological activity loss is low, and the purities of the sargassum graminifolium polysaccharide and the sargassum graminifolium polyphenol are high; meanwhile, time is short, handing capacity is high, continuous operation can be realized, cost is low, and clean environment can be realized, so that the method for synchronously extracting the sargassum graminifolium polysaccharide and the sargassum graminifolium polyphenol from the sargassum graminifolium is especially applicable to industrial application.

Description

technical field

[0001] The invention relates to sargassum polysaccharides and sargassum polyphenols in the field of medicines and health products, in particular to a method for synchronously extracting sargassum sargassum polysaccharides and sargassum sargassum polyphenols from sargassum sargassum. Background technique

[0002] Sargassum (Sargassum) is a plant of the Sargassum family of Phaeolophyta Staghornaceae. Sargassum.graminifolium.turn belongs to the genus Sargassum. It grows on rocky reefs in the low tidal zone and subtidal zone. The algal body is green or brown and about 50 cm high. It is distributed in Zhoushan and Xiangshan, Zhejiang in my country. , Fujian, Guangdong and Macao are rich in resources.

[0003] At present, the scope of processing and utilization of Sargassum is relatively narrow, only a small part is used as feed and raw material of alginate, some are edible, and most of them have not been fully developed and utilized. Sargassum, like other types o...

Examples

Embodiment 1

[0053] Embodiment 1 The method (1) of synchronously extracting Sargassum grassa polysaccharides and Sargassum grassa polyphenols from Sargassum grassa

[0054] 1. Method steps

[0055] 1.1 Simultaneous extraction of polysaccharides and polyphenols from Sargassum sargassum

[0056] (1) Remove impurities from the raw material Sargassum sargassum and wash it with water, put the cleaned Sargassum sargassum into a drying box, dry at 30°C for 20 hours to constant weight, and then put the dried Sargassum sargassum in Pulverize in a pulverizer to obtain powder, pass through a 20-mesh sieve to obtain dry powder of Sargassum grassa, and freeze for future use.

[0057] (2) Add ethanol with a volume fraction of 80% to the dry powder of Sargassum sargassum, and then reflux for extraction for about 3 hours. After the reflux, filter out the ethanol, then place the Sargassum sargassum powder in a fume hood to completely volatilize the ethanol, and finally dry the Sargassum sargassum powder ...

Embodiment 2

[0092] Embodiment 2 The method of synchronously extracting polysaccharides and polyphenols from Sargassum sargassum from Sargassum sargassum of the present invention (two)

[0093] 1. Method steps

[0094] (1) Remove impurities from the raw material Sargassum sargassum and wash it with water, put the cleaned Sargassum sargassum into a drying box, dry at 20°C for 24 hours to constant weight, and then put the dried Sargassum sargassum in Pulverize in a pulverizer to obtain powder, pass through a 40-mesh sieve to obtain dry powder of Sargassum grassa, and freeze for future use.

[0095] (2) Add ethanol with a volume fraction of 90% to the dry powder of Sargassum sargassum, and then reflux for extraction for about 2 hours. After the reflux, filter out the ethanol, then place the Sargassum sargassum powder in a fume hood to completely volatilize the ethanol, and finally dry the Sargassum sargassum powder in a drying oven at a drying temperature of 20°C until it reaches a constant ...

Embodiment 3

[0109] Embodiment 3 The method of synchronously extracting polysaccharides and polyphenols from Sargassum sargassum from Sargassum sargassum of the present invention (three)

[0110] 1. Method steps

[0111] (1) Remove impurities from the raw material Sargassum sargassum and wash it with water, put the cleaned Sargassum sargassum into a drying box, dry at 40°C for 12 hours to constant weight, and then put the dried Sargassum sargassum in Pulverize in a pulverizer to obtain powder, pass through a 10-mesh sieve to obtain dry powder of Sargassum pratense, and freeze for future use.

[0112] (2) Add ethanol with a volume fraction of 70% to the dry powder of Sargassum sargassum, and then reflux for extraction for about 4 hours. After the reflux, filter out the ethanol, then place the Sargassum sargassum powder in a fume hood to completely volatilize the ethanol, and finally dry the Sargassum sargassum powder in a drying oven at a drying temperature of 40°C until it reaches a const...

technical field

[0001] The invention relates to sargassum polysaccharides and sargassum polyphenols in the field of medicines and health products, in particular to a method for synchronously extracting sargassum sargassum polysaccharides and sargassum sargassum polyphenols from sargassum sargassum. Background technique

[0002] Sargassum (Sargassum) is a plant of the Sargassum family of Phaeolophyta Staghornaceae. Sargassum.graminifolium.turn belongs to the genus Sargassum. It grows on rocky reefs in the low tidal zone and subtidal zone. The algal body is green or brown and about 50 cm high. It is distributed in Zhoushan and Xiangshan, Zhejiang in my country. , Fujian, Guangdong and Macao are rich in resources.

[0003] At present, the scope of processing and utilization of Sargassum is relatively narrow, only a small part is used as feed and raw material of alginate, some are edible, and most of them have not been fully developed and utilized. Sargassum, like other types o...

Examples

Embodiment 1

[0053] Embodiment 1 The method (1) of synchronously extracting Sargassum grassa polysaccharides and Sargassum grassa polyphenols from Sargassum grassa

[0054] 1. Method steps

[0055] 1.1 Simultaneous extraction of polysaccharides and polyphenols from Sargassum sargassum

[0056] (1) Remove impurities from the raw material Sargassum sargassum and wash it with water, put the cleaned Sargassum sargassum into a drying box, dry at 30°C for 20 hours to constant weight, and then put the dried Sargassum sargassum in Pulverize in a pulverizer to obtain powder, pass through a 20-mesh sieve to obtain dry powder of Sargassum grassa, and freeze for future use.

[0057] (2) Add ethanol with a volume fraction of 80% to the dry powder of Sargassum sargassum, and then reflux for extraction for about 3 hours. After the reflux, filter out the ethanol, then place the Sargassum sargassum powder in a fume hood to completely volatilize the ethanol, and finally dry the Sargassum sargassum powder ...

Embodiment 2

[0092] Embodiment 2 The method of synchronously extracting polysaccharides and polyphenols from Sargassum sargassum from Sargassum sargassum of the present invention (two)

[0093] 1. Method steps

[0094] (1) Remove impurities from the raw material Sargassum sargassum and wash it with water, put the cleaned Sargassum sargassum into a drying box, dry at 20°C for 24 hours to constant weight, and then put the dried Sargassum sargassum in Pulverize in a pulverizer to obtain powder, pass through a 40-mesh sieve to obtain dry powder of Sargassum grassa, and freeze for future use.

[0095] (2) Add ethanol with a volume fraction of 90% to the dry powder of Sargassum sargassum, and then reflux for extraction for about 2 hours. After the reflux, filter out the ethanol, then place the Sargassum sargassum powder in a fume hood to completely volatilize the ethanol, and finally dry the Sargassum sargassum powder in a drying oven at a drying temperature of 20°C until it reaches a constant ...

Embodiment 3

[0109] Embodiment 3 The method of synchronously extracting polysaccharides and polyphenols from Sargassum sargassum from Sargassum sargassum of the present invention (three)

[0110] 1. Method steps

[0111] (1) Remove impurities from the raw material Sargassum sargassum and wash it with water, put the cleaned Sargassum sargassum into a drying box, dry at 40°C for 12 hours to constant weight, and then put the dried Sargassum sargassum in Pulverize in a pulverizer to obtain powder, pass through a 10-mesh sieve to obtain dry powder of Sargassum pratense, and freeze for future use.

[0112] (2) Add ethanol with a volume fraction of 70% to the dry powder of Sargassum sargassum, and then reflux for extraction for about 4 hours. After the reflux, filter out the ethanol, then place the Sargassum sargassum powder in a fume hood to completely volatilize the ethanol, and finally dry the Sargassum sargassum powder in a drying oven at a drying temperature of 40°C until it reaches a const...