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Construction of novel sugar alcohol-producing yeast strain genome rearrangement technology and application thereof

A technology of genome rearrangement and yeast strains, applied in the direction of microorganism-based methods, fungi, and treatment of microorganisms with electricity/wave energy, to achieve low cost, high-efficiency fusion screening, and high safety factor

Active Publication Date: 2015-08-12
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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Problems solved by technology

However, the high-yield, high-tolerance and other excellent characteristics of industrial strains are jointly determined by a large number of genes, and most of the problems faced by the improvement of industrial microbial strains

Method used

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  • Construction of novel sugar alcohol-producing yeast strain genome rearrangement technology and application thereof
  • Construction of novel sugar alcohol-producing yeast strain genome rearrangement technology and application thereof
  • Construction of novel sugar alcohol-producing yeast strain genome rearrangement technology and application thereof

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Experimental program
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Effect test

Embodiment

[0030] Application of a new genome rearrangement technology in the improvement of the sugar alcohol-producing strain Pichia anomaly:

[0031] In order to further effectively improve the production performance of yeast sugar alcohols, the strains (U-7, 9 and A-1, 4) obtained by traditional mutagenesis breeding methods were used as the starting bacteria, and the new genome rearrangement technology was used to construct different high-yielding sugar alcohols. The strains are combined and rearranged, and the strains with excellent performance are screened. The specific process is as follows: image 3 . First, the strains were activated and cultured in YPD liquid medium for 12 hours to the logarithmic growth phase, then the cell wall was removed, and 2.5% helicase solution was selected for treatment at 30°C for 3 hours, and observed by microscopy and plate coating. Protoplast formation rate and regeneration rate. Next, the prepared protoplast cells were labeled with different flu...

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Abstract

A novel sugar alcohol-producing yeast strain genome rearrangement technology mainly includes high throughput screening of a sugar alcohol-producing yeast strain on the basis of a color development method and fusion cell high-efficient flow sorting on the basis of bi-fluorescent staining. By means of the technology, high-effective genome rearrangement and screening of alcohol-producing yeast are achieved. The technology is simple, low-cost, high-efficient and practical. Through the technology, high-efficient and quick improvement on natural pichia pastoris being abnormal in sugar alcohol producing can be successfully achieved, thereby increasing the yield of sugar alcohol by more than 34%.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a novel genome rearrangement technology of sugar alcohol-producing yeast strains and the technology used in producing sugar alcohol-producing yeast Pichia anomaly ( Pichia anomala ) applications in genome modification. Background technique [0002] Sugar alcohols refer to the polyols formed after the aldehyde and ketone carbonyl groups of sugars are reduced to hydroxyl groups, with the general formula H(CHOH) n+1 H, which loses its reductibility due to hydrogenation, has a hydroxyl group attached to each carbon atom. They have many unique properties, such as low metabolic energy in the body, can prevent dental caries, and have low insulin metabolic response. Therefore, they are an important class of functional food raw materials. In addition, sugar alcohols also have more applications in the fields of medicine, chemical industry and animal nutrition. In the fie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12N13/00C12N15/04C12N1/16C12N1/19C12R1/84
Inventor 王钦宏张国强贺鹏
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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