Method of screening strains for producing microorganism plastics
A technology for microorganisms and plastics, applied in the field of screening microbial plastic strains based on single-cell Raman spectroscopy, can solve the problems of poor specificity, time-consuming, cumbersome operation methods, etc., and achieve good signal-to-noise ratio and easy operation
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[0028] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. This embodiment is only to illustrate the present invention more clearly, rather than to limit the present invention.
[0029] 1. Correlation Analysis of Colony Cell Raman Spectrum and Shake Flask Fermentation PHB Yield
[0030] 1. Experimental materials
[0031] Strains: Cupriavidus necator H16 strain.
[0032] Incline medium: yeast powder 10g / L, peptone 10g / L, beef extract 5g / L, NaCl 5g / L, agar 20g / L, pH 7.0.
[0033] Seed medium: yeast powder 10 g / L, peptone 10 g / L, beef extract 5 g / L, NaCl 5 g / L, pH 7.0.
[0034] Shake flask fermentation medium: Na 2 HPO 4 12H 2 O 9.0 g / L, KH 2 PO 4 1.5 g / L, MgSO 4 ·7H 2 O 0.2 g / L, CaCl 2 2H 2 O 0.02 g / L, NaHCO 30.5 g / L, ferric ammonium citrate 0.05 g / L, (NH4) 2 SO 4 2.0 g / L, trace element solution 1 mL. The pH value is 7.0, and it is sterilized at 115 ℃ for 20 minutes.
[0035] Trace ele...
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