Preparation method of melaleuca alternefolia total flavone and detection method thereof

A technology of Melaleuca alternifolia and total flavonoids is applied in the field of preparation of flavonoid extracts, which can solve the problems of insufficient development and utilization of plants, waste of medicinal material resources and the like, and achieves reasonable design, good application prospects and low energy consumption. Effect

Active Publication Date: 2015-08-19
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

) The branches and leaves of Melaleuca alternifolia are extracted by water distillation to extract essential oils, which are usually treated as production w...

Method used

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  • Preparation method of melaleuca alternefolia total flavone and detection method thereof
  • Preparation method of melaleuca alternefolia total flavone and detection method thereof
  • Preparation method of melaleuca alternefolia total flavone and detection method thereof

Examples

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Embodiment 1

[0031]Take 1000g of Melaleuca alternifolia branches and leaves, add 3L of water, heat for 70 minutes, extract the volatile oil, after the oil is collected, discard the aqueous solution in the container, add 12L of 75% ethanol, heat and reflux to extract 3 times, each time for 2 hours, and combine the extracts , filtered, and the filtrate was recovered under reduced pressure until it had no ethanol smell, dissolved in water, and made into an extract of Melaleuca alternifolia with a total flavonoid concentration of 0.5 mg / mL. Take 80 g of the processed X-5 macroporous adsorption resin (equivalent to 30 g of dry resin, column volume 200 mL), and wet-pack the column, and put 600 mL of Melaleuca alternifolia extract at 3 BV / h Adsorb at the column flow rate, elute with 3 BV of water until the eluate is basically clear, discard the water eluate, elute with 4BV 75% ethanol at a flow rate of 2BV / h, collect the eluate, and recover ethanol under reduced pressure. 5%。 Concentrated to dryn...

Embodiment 2

[0033] Take 1200g of Melaleuca alternifolia branches and leaves, add 4L of water, heat for 70 minutes, extract the volatile oil, after the oil is collected, discard the aqueous solution in the container, add 15L of 50% ethanol, heat and reflux for extraction 3 times, each time for 3 hours, and combine the extracts , filtered, and the filtrate was recovered under reduced pressure until it had no ethanol smell, dissolved in water, and made into an extract of Melaleuca alternifolia with a total flavonoid concentration of 0.8 mg / mL. Get 60g of X-5 macroporous adsorption resin that has been processed (equivalent to 20g of dry resin, column volume 150mL), wet packing column, 750mL Melaleuca alternifolia extract is adsorbed with the upper column flow rate of 2BV / h, Elute with 4BV of water until the eluate is basically clear, discard the water eluate, elute with 5BV of 50% ethanol at a flow rate of 4BV / h, collect the eluate, recover ethanol under reduced pressure, concentrate to drynes...

Embodiment 3

[0035] Take 800g of Melaleuca alternifolia branches and leaves, add 2L of water, heat for 70 minutes, extract the volatile oil, after the oil is collected, discard the aqueous solution in the container, add 8L of 95% ethanol, heat and reflux for extraction 3 times, each time for 1 hour, and combine the extracts , filtered, and the filtrate was recovered under reduced pressure until it had no ethanol smell, dissolved in water, and made into an extract of Melaleuca alternifolia with a total flavonoid concentration of 0.2 mg / mL. Take 50g of the treated AB-8 macroporous adsorption resin (equivalent to 20g of dry resin, column volume 150mL), wet-pack the column, and absorb 450mL of Melaleuca alternifolia extract at a column flow rate of 0.5BV / h , eluted with 4BV of water until the eluate was basically clear, discarded the water eluate, eluted with 3BV of 80% ethanol at a flow rate of 5BV / h, collected the eluate, recovered ethanol under reduced pressure, concentrated to dryness, 60 ...

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Abstract

The invention discloses melaleuca alternefolia total flavone extract and a preparation method thereof. The preparation method comprises the following steps of extracting essence oil from the melaleuca alternefolia branch and leave by water vapor, performing ethanol heating and refluxing extraction, reducing the pressure to recycle the ethanol, concentrating to remove the ethanol flavor, separating and purifying by macroporous absorption resin, and drying; measuring the content of total flavone in the melaleuca alternefolia by adopting an ultraviolet-visible spectrophotometry method. The preparation method has the advantages that the method is accurate and stable, the operation is simple, the requirement of industrial production is met, and the quality of the melaleuca alternefolia total flavone extract can be effectively controlled; the content of total flavone in the melaleuca alternefolia total flavone extract can reach more than 50 percent, the toxicity is little, and the pharmacological functions of resisting inflammation, inhibiting bacteria, alleviating pain, resisting allergy, resisting oxidizing and the like are realized; the preparation method is reasonable and stable, the production cycle is short, the preparation method is suitable for industrial production, the important meaning is realized on the deep development and utilization of melaleuca alternefolia resources, and the development prospect is good.

Description

technical field [0001] The invention relates to the field of preparation of flavonoid extracts, in particular to a purification preparation and detection method of total flavonoids of Melaleuca alternifolia. Background technique [0002] Natural plant extracts use plants as raw materials, according to the purpose and needs of the extraction, use physical and chemical extraction and separation methods to obtain and concentrate one or more active ingredients in the plant without changing the structure of the active ingredients. formed products. Compared with traditional Chinese herbal medicines and proprietary Chinese medicines, Chinese herbal extracts have clear active ingredients, quantitative indicators for quality control, and most of the harmful ingredients or impurities are removed. They are more safe, effective, and controllable than traditional herbal medicines. get promoted. In addition, traditional Chinese medicine extracts also incorporate new modern pharmaceutica...

Claims

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Application Information

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IPC IPC(8): A61K36/61A61P29/00A61P31/04A61P37/08A61P39/06G01N21/31A61K127/00A61K135/00
Inventor 刘布鸣林霄邱宏聪柴玲黄艳
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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