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Separation and extraction method of disporum cantoniense rhizome and root polysaccharide

An extraction method and ginseng technology are applied in the field of separation and extraction of ginseng polysaccharides to achieve the effects of easy operation, good quality and high purity

Inactive Publication Date: 2015-08-19
GUIZHOU EDUCATION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the related research on polysaccharides of ginseng

Method used

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  • Separation and extraction method of disporum cantoniense rhizome and root polysaccharide
  • Separation and extraction method of disporum cantoniense rhizome and root polysaccharide
  • Separation and extraction method of disporum cantoniense rhizome and root polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] A kind of ginseng polysaccharide is obtained from ginseng by the following method:

[0064] (1) Pretreatment: Wash the fresh ginseng, dry it at 40°C and crush it;

[0065] (2) Degreasing: Put the powdered ginseng and petroleum ether in a Soxhlet extractor at a material-to-liquid ratio of 1:50, and reflux at 50°C for 3 hours to obtain degreased ginseng powder;

[0066] (3) Extraction: add distilled water to the degreased ginseng powder, the ratio of solid to liquid is 1:20, and extract once an hour at 70°C;

[0067] (4) Separation: Suction filtration, alcohol precipitation of the filtrate and 95% ethanol at a ratio of 1:3.5, let it stand for 6 hours; centrifuge at 4000r / min for 20 minutes, remove the supernatant, and distill the precipitation to 500ml with distilled water;

[0068] (5) AB-8 resin was decolorized and purified, concentrated to 1 / 3 of the original volume to obtain crude polysaccharide liquid;

[0069] (6) Deproteinization: The crude polysaccharide solutio...

Embodiment 2

[0072] A kind of ginseng polysaccharide is obtained from ginseng by the following method:

[0073] (1) Pretreatment: Wash the fresh ginseng, dry it at 60°C and crush it;

[0074] (2) Degreasing: Put the powdered ginseng and petroleum ether in a Soxhlet extractor at a material-to-liquid ratio of 1:50, and reflux at 50°C for 3 hours to obtain degreased ginseng powder;

[0075] (3) Extraction: add distilled water to the degreased ginseng powder, the ratio of solid to liquid is 1:80, and extract 4 times at 100°C for 4 hours each time;

[0076] (4) Separation: suction filtration, alcohol precipitation of the filtrate and 95% ethanol at a ratio of 1:4, let it stand for 8 hours; centrifuge at 3000r / min for 15 minutes, remove the supernatant, and distill the precipitation to 500ml with distilled water;

[0077] (5) AB-8 resin was decolorized and purified, concentrated to 1 / 3 of the original volume to obtain crude polysaccharide liquid;

[0078] (6) Deproteinization: The crude polysa...

Embodiment 3

[0081] A kind of ginseng polysaccharide is obtained from ginseng by the following method:

[0082] (1) Pretreatment: Wash the fresh ginseng, dry it and crush it;

[0083] (2) Degreasing: Put the powdered ginseng and petroleum ether in a Soxhlet extractor at a material-to-liquid ratio of 1:50, and reflux at 50°C for 3 hours to obtain degreased ginseng powder;

[0084] (3) Extraction: add distilled water to the degreased ginseng powder, the ratio of solid to liquid is 1:60, and extract twice at 85°C for 3 hours each time;

[0085] (4) Separation: suction filtration, alcohol precipitation of the filtrate and 95% ethanol at a ratio of 1:3.5, let it stand for 7 hours; centrifuge at 3500r / min for 17 minutes, remove the supernatant, and distill the precipitation to 500ml with distilled water;

[0086] (5) AB-8 resin was decolorized and purified, concentrated to 1 / 3 of the original volume to obtain crude polysaccharide liquid;

[0087] (6) Deproteinization: The crude polysaccharide ...

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Abstract

The invention discloses a separation and extraction method of disporum cantoniense rhizome and root polysaccharide. The separation and extraction method comprises the steps of washing, drying and grinding fresh disporum cantoniense rhizomes and roots; carrying out reflux degreasing on the ground fresh disporum cantoniense rhizomes and roots and petroleum ether; adding distilled water to extract, combining water extracts after extraction is finished; carrying out extraction filtration on the combined water extract by using a Buchner funnel, carrying out alcohol precipitation on filtrate, standing, centrifuging and removing supernatant, and making the volume of precipitates to be constant by using distilled water; carrying out decoloration, purification and concentration to obtain a coarse polysaccharide solution; adding the mixed solution of chloroform and n-butyl alcohol into the coarse polysaccharide solution according to a mass ratio of the chloroform to the n-butyl alcohol being 3:1, shaking out, then taking supernatant, adding ethanol, standing and centrifuging to obtain deprotein disporum cantoniense rhizome and root polysaccharide; washing, freezing and drying to obtain the disporum cantoniense rhizome and root polysaccharide. According to the separation and extraction method disclosed by the invention, the disporum cantoniense rhizome and root polysaccharide with high purity and high quality can be obtained; the method is simple easy to operate, and has extremely high market popularization value.

Description

technical field [0001] The invention belongs to the technical field of natural medicines, and in particular relates to a method for separating and extracting polysaccharides from Radix ginseng. Background technique [0002] Hundred-tailed ginseng belongs to the Liliaceae ( Liliaceae ) Wanshou Bamboo ( Disposal ) Plant Longevity Bamboo ( Disporum cantoniense (Lour.) Merr. ) roots and rhizomes, also known as white ginseng, hundred-tailed bamboo shoots, and white dragon beard. Ginseng is a perennial herbaceous plant, which is a traditional medicinal material of the Miao nationality. It is sweet, light, and mild in nature. , Food accumulation and fullness and other symptoms. [0003] The marketed compound preparations with Baiwei ginseng as the main drug include Kesuting syrup and capsules produced by Guizhou Bailing Enterprise Group Pharmaceutical Co., Ltd. In terms of component research, Chen Lei et al. used chemical separation methods and modern spectrum technology t...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 曹剑锋任朝辉刘丹夏丽莎庞宇云兰世超
Owner GUIZHOU EDUCATION UNIV
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